International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Efficient Production of Porcine Circovirus Type 2 Capsid Protein using Baculovirus

  • Lee, Jun-Beom (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Bae, Sung-Min (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Kim, Hee-Jung (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Lee, Won-Woo (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Heo, Won-Il (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Shin, Tae-Young (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Choi, Jae-Bang (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Woo, Soo-Dong (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
  • Received : 2012.01.28
  • Accepted : 2012.03.08
  • Published : 2012.03.31
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Abstract

Porcine circovirus type 2 (PCV2) is a single-stranded circular DNA virus associated with Postweaning multisystemic wasting syndrome (PMWS), which is considered to be an important infectious swine viral disease. PCV2 capsid protein encoded by ORF2 is a structural protein and expected as the high immunogenicity protein. In this study, we generated recombinant baculovirus containing ORF2 of PCV2 and analyzed the optimal conditions for the production of capsid protein in insect cell. Production and status of recombinant capsid protein in insect cell were confirmed by SDS-PAGE and Western blot analysis using His tag antibody and anti-PCV2 serum. The yield of recombinant capsid protein was high like as shown visible on SDS-PAGE. Optimal multiplicity of infection (MOI) and infection time of recombinant virus were determined as 5 MOI and 4 days, respectively. ORF2 is known to have N-linked glycosylation site, but we couldn't detect the glycosylation of recombinant protein in insect cells.

Keywords

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