Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Morphological Changes of Fungal Cell Wall and ABC Transporter as Resistance Responses of Rice Bakanae Disease Pathogen Fusarium fujikuroi CF337 to Prochloraz

세포벽의 형태학적 변화와 ABC Transporter에 기초한 벼키다리병원균 Fusarium fujikuroi CF337의 살균제 prochloraz에 대한 저항성 반응

  • Yang, You-Ri (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Si-Woo (Division of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Se-Won (Division of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, In-Seon (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University)
  • 양유리 (전남대학교 농업생명과학대학 친환경농업연구소) ;
  • 이시우 (국립농업과학원 농업생물부) ;
  • 이세원 (국립농업과학원 농업생물부) ;
  • 김인선 (전남대학교 농업생명과학대학 친환경농업연구소)
  • Received : 2012.03.03
  • Accepted : 2012.03.21
  • Published : 2012.03.31
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Abstract

BACKGROUND: The resistance of rice bakanae disease pathogens against the fungicide prochloraz has been reported. Understanding the resistance mechanisms is an important for better control of the pathogens. In the present study, we investigated the resistance mechanisms of Fusarium fujikuroi CF337 (CF337) against prochloraz. METHODS AND RESULTS: Morphological changes in the cell wall of CF337 grown in potato dextrose broth (PDB) with or without prochloraz was investigated by transmission electron microscopy. Growth inhibition of CF337 was examined in PDB containing prochloraz or an ABC transporter inhibitor or both of them. Cell wall thickness of CF337 grown in PDB with prochloraz was significantly increased from $80.73{\pm}1.99nm$ to $193.11{\pm}7.07nm$. Significant inhibition in the growth of CF337 was observed in the presence of both prochloraz and the inhibitor, but no growth inhibition was observed in the presence of the inhibitor or prochloraz. Sequence analysis of ATP-binding cassette transporter (ABC) gene of CF337 showed 70 to 80% similarities to the genes of the pathogens resistant to other fungicides. CONCLUSION: Efflux transporter system and changes in cell wall thickness were suggested as resistance mechanisms of CF337 against prochloraz.

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References

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