Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Growth Response to a GH-Autotransgenesis in Common Carp Cyprinus carpio

  • Noh, Choong-Hwan (Living Resources Laboratory, Korea Ocean Research and Development Institute) ;
  • Kim, Dong-Soo (Department of Marine Bio-Materials and Aquaculture, Pukyong National University)
  • Received : 2011.09.05
  • Accepted : 2011.11.29
  • Published : 2012.03.30
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Abstract

Autotransgenic manipulation with a growth hormone (GH)-construct is a potential approach to improving the growth rates of farmed fish. Here, we present the generation of GH-autotransgenic common carp Cyprinus carpio carrying a transgene comprised of the carp homologous GH gene and a ${\beta}$-actin regulator. Autotransgenic carp showed similar viability to their non-transgenic siblings. Early growth characteristics of founder autotransgenic carp up to 50 days postfertilization were highly variable among individuals; i.e., some fish exhibited significant growth depression, while others showed dramatic acceleration of growth, achieving greater than sixfold increases in body weight relative to their non-transgenic counterparts. Stimulated growth performance became more notable with age and many transgenic individuals of the largest class reached 5 kg within 8 or 9 months, which is at least 10 times heavier than the average body weight of communally grown non-transgenics. Four of six founder transgenic males were successful in passing the transgene to their $F_1$ offspring with frequencies ranging from 19 to 36%. Growth stimulations were also persistent in all $F_1$ progeny groups examined.

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References

  1. Chen TT, Kight K, Lin CM, Powers DA, Hayat M, Chatakondi N, Ramboux AC, Duncan PL and Dunham RA. 1993. Expression and inheritance of RSVLTR-rtGH1 complementary DNA in the transgenic common carp, Cyprinus carpio. Mol Mar Biol Biotechnol 2, 88-95.
  2. Devlin RH, Yesaki TY, Biagi CA, Donaldson EM, Swanson P and Chan WK. 1994. Extraordinary salmon growth. Nature 371, 209-210. https://doi.org/10.1038/371209a0
  3. Devlin RH, Yesaki YT, Donaldson EM, Du SJ and Hew CL. 1995. Production of germline transgenic Pacific salmonids with dramatically increased growth performance. Can J Fish Aquat Sci 52, 1376-1384. https://doi.org/10.1139/f95-133
  4. Devlin RH, Sundström LF and Muir WM. 2006. Interface of biotechnology and ecology for environmental risk assessments of transgenic fish. Trends Biotechnol 24, 89-97. https://doi.org/10.1016/j.tibtech.2005.12.008
  5. Fu C, Hu W, Wang Y and Zhu Z. 2005. Developments in transgenic fish in the People's Republic of China. Rev Sci Tech Off Int Epizoot 24, 299-307. https://doi.org/10.20506/rst.24.1.1577
  6. Gong Z, Maclean N, Devlin RH, Martinez R, Omitogun O and Estrada MP. 2007. Gene construct and expression: information relevant for risk assessment and management. In: Environment Risk Assessment of Genetically Modified Organisms. Vol. 3. Methodologies for Transgenic Fish. Kapuscinski AR, Hayes KR, Li S and Dana G, eds. CABI Publishing, Oxford, GB, pp. 95-111.
  7. Hackett PB and Alvarez MC. 2000. The molecular genetics of transgenic fish. In: Recent Advances in Marine Biotechnology. Vol. 4. Fingerman M and Nagabhushanam R, eds. Science Publishers Inc., Enfield, NH, US, pp. 77-145.
  8. Hinits Y and Moav B. 1999. Growth performance studies in transgenic Cyprinus carpio. Aquaculture 173, 285-296. https://doi.org/10.1016/S0044-8486(98)00452-9
  9. Kapuscinski AR. 2005. Current scientific understanding of the environmental biosafety of transgenic fish and shellfish. Rev Sci Tech Off Int Epizoot 24, 309-322. https://doi.org/10.20506/rst.24.1.1576
  10. Maclean N. 2003. Genetically modified fish and their effects on food quality and human health and nutrition. Trends Food Sci Technol 14, 242-252. https://doi.org/10.1016/S0924-2244(03)00070-0
  11. Maclean N, Penman D and Zhu Z. 1987. Introduction of novel genes into fish. Biotechnology 5, 257-261. https://doi.org/10.1038/nbt0387-257
  12. Nam YK, Noh JK, Cho YS, Cho HJ, Cho KN, Kim CG and Kim DS. 2001. Dramatically accelerated growth and extraordinary gigantism of transgenic mud loach Misgurnus mizolepis. Transgenic Res 10, 353-362. https://doi.org/10.1023/A:1016696104185
  13. Nam YK, Cho YS, Cho HJ and Kim DS. 2002. Accelerated growth performance and stable germ-line transmission in androgenetically derived homozygous transgenic mud loach, Misgurnus mizolepis. Aquaculture 209, 257-270. https://doi.org/10.1016/S0044-8486(01)00730-X
  14. Nam YK, Park IS and Kim DS. 2004. Triploid hybridization of fast-growing transgenic mud loach Misgurnus mizolepis male to cyprinid loach Misgurnus anguillicaudatus female: the first performance study on growth and reproduction of transgenic polyploid hybrid fish. Aquaculture 231, 559-572. https://doi.org/10.1016/j.aquaculture.2003.09.046
  15. Nam YK, Maclean N, Fu C, Pandian TJ and Eguia MRR. 2007. Development of transgenic fish: scientific background. In: Environment Risk Assessment of Genetically Modified Organisms. Vol. 3. Methodologies for Transgenic Fish. Kapuscinski AR, Hayes KR, Li S and Dana G, eds. CABI Publishing, Oxford, GB, pp. 61-94.
  16. Nam YK, Maclean N, Hwang G and Kim DS. 2008. Autotransgenic and allotransgenic manipulation of growth traits in fish for aquaculture: a review. J Fish Biol 72, 1-26. https://doi.org/10.1111/j.1095-8649.2007.01738.x
  17. Rajesh R and Majumdar KC. 2005. Transgene integration: an analysis in autotransgenic Labeo rohita Hamilton (Pisces: Cyprinidae). Fish Physiol Biochem 31, 282-287.
  18. Wu G, Sun Y and Zhu Z. 2003. Growth hormone gene transfer in common carp. Aquat Living Resour 16, 416-420. https://doi.org/10.1016/S0990-7440(03)00087-1
  19. Zbikowska HM. 2003. Fish can be first: advances in fish transgenesis for commercial applications. Transgenic Res 12, 379-389. https://doi.org/10.1023/A:1024267416522