Transactions of the Korean Society of Mechanical Engineers B (대한기계학회논문집B)

The Korean Society of Mechanical Engineers (대한기계학회)
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A Continuous Cell Separator Based on Gravity and Buoyant Forces in Fluids of Dissimilar Density

서로 다른 밀도의 유체 내 바이오 물질이 받는 중력과 부력 차를 이용한 연속적 세포 분리기

  • Oh, Ae-Gyoung (KAIST, Cell Bench Research Center, Dept. of Bio and Brain Engineering) ;
  • Lee, Dong-Woo (KAIST, Cell Bench Research Center, Dept. of Bio and Brain Engineering) ;
  • Cho, Young-Ho (KAIST, Cell Bench Research Center, Dept. of Bio and Brain Engineering)
  • 오애경 (한국과학기술원 세포벤치연구센터 바이오및뇌공학과) ;
  • 이동우 (한국과학기술원 세포벤치연구센터 바이오및뇌공학과) ;
  • 조영호 (한국과학기술원 세포벤치연구센터 바이오및뇌공학과)
  • Received : 2011.07.27
  • Accepted : 2012.01.19
  • Published : 2012.04.01
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Abstract

We present a continuous cell separator that achieves density-dependent and size-independent cell separation based on the net force of gravity and buoyancy forces on the cells in dissimilar density fluids. Previous cell separators are, based on the size or dielectrophoretic property of the cells and, are suitable for size-dependent and density-independent cell separation. However, these properties can make it difficult to collect the same types of cells with the same density but with size variations. The present separator, however, is capable of collecting the same types of cells based on the cell density in the fluid. Regardless of cell size, the proposed chip isolates low density cells, (white blood cells, or WBCs) at the upper outlet while obtaining high-density cells (red blood cells, or RBCs) from the lower outlet based on density. Efficiency levels for separation of WBCs and RBCs were $90.9{\pm}9.1%$ and $86.4{\pm}1.99%$, respectively. The present separator therefore has the potential for use in the pretreatment of whole blood.

본 논문에서는 서로 다른 밀도의 유체 내 바이오 물질이 받는 중력과 부력 차를 이용한 연속적 세포 분리기를 제안하였다. 종래의 크기별 세포분리는 서로 다른 크기의 동일한 밀도를 가지는 세포를 분리하는데 한계가 있다. 반면, 본 논문에서 제안하는 세포 분리기는 미소유로 상하부에 밀도가 다른 다층 유체층 내에서 세포가 받는 중력과 부력 차이로 크기는 다르지만 동일한 밀도를 가지는 세포를 효율적으로 분리할 수 있다. 밀도가 다른 유체층(PBS, 밀도=1.0g/ml, Ficoll, 밀도=1.1g/ml) 내에서 전혈로부터 백혈구(직경=$6-10{\mu}m$, 밀도=1.06~1.1g/ml), 적혈구(직경=$4-6{\mu}m$, 밀도=1.09~1.2g/ml)를 밀도에 따라 분리한 효율이 각각 $90.9{\pm}9.1%$와 $86.4{\pm}1.99%$로 측정되었다 따라서, 본 세포 분리기는 크기 편차가 있는 동일 밀도의 세포를 크기에 둔감하고 밀도에만 민감한 분리가 가능하다.

Keywords

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