DOI QR코드

DOI QR Code

부착성 사상세균 Leucothrix mucor 배양액에서 분리된 Giffinisterone B와 Oleamide의 해조류 Ulva pertusa 부착방지 효과

Antifouling Activity of Giffinisterone B and Oleamide Isolated from a Filamentous Bacterium Leucothrix mucor Culture against Ulva pertusa

  • 조지영 (순천향대학교 해양생명공학과)
  • Cho, Ji-Young (Department of Marine Biotechnology, Soonchunhyang University)
  • 투고 : 2011.12.23
  • 심사 : 2012.02.03
  • 발행 : 2012.02.29

초록

The filamentous bacterium Leucothrix mucor, an epiphyte of seaweed, showed antifouling activity against Ulva pertusa spore settlement and germling development. The chemical constituents representing the antifouling activity were identified as giffinisterone B and oleamide based on nuclear magnetic resonance (NMR) spectroscopy and mass spectroscopy (MS). Approximately 3.6 mg of giffinisterone B and 2.8 mg of oleamide were isolated from 1.6 g of Leucothrix mucor crude extract. Giffinisterone B fully inhibited spore settlement and germling development at $100{\mu}g/mL$. Oleamide inhibited spore settlement at $10{\mu}g/mL$ and germling development at $100{\mu}g/mL$.

키워드

참고문헌

  1. Ahlgren MO. 1998. Consumption and assimilation of salmon net pen fouling debris by the Red Sea cucumber Parastichpus clifornicus: implications for polyculture. J World Aquac Soc 29, 133-139. https://doi.org/10.1111/j.1749-7345.1998.tb00972.x
  2. Akanmu MA, Adeosun SO and Ilesanmi OR. 2007. Neuropharmacological effects of oleamide in male and female mice. Behavioural Brain Research 182, 88-94. https://doi.org/10.1016/j.bbr.2007.05.006
  3. Beveridge M. 2004. Cage Aquaculture. In: Problems. Blackwell Publishing Ltd, Oxford, U.K., 251-255
  4. Bhattarai HD, Ganti VS, Paudel B, Lee YK, Lee HK, Hong YK and Shin HW. 2007. Isolation of antifouling compounds from the marine bacterium, Shewanella oneidensis SCH0402, World J Microbiol Biotechnol 23, 243-249. https://doi.org/10.1007/s11274-006-9220-7
  5. Brock TD. 1966. The habitat of Leucothrix mucor, a widespread marine organism. Lim-nol. Oceanogr 11, 303-307. https://doi.org/10.4319/lo.1966.11.2.0303
  6. Callow ME and Edyvean RGV. 1990. Algal fouling and corrosion. In : Introducion to applied phycology. Akatsuka I, ed. SPB Academic Publishing, Netherlands, 367-387
  7. Chao CH, Wen ZH, Chen IM, Su JH, Huang HC, Chiang MY and Sheu JH. 2008. Anti-inflammatory steroids from the octocoral Dendronephthya griffin. Tetrahedron 64, 3554- 3560 https://doi.org/10.1016/j.tet.2008.01.109
  8. Cho JY, Kwon EH, Choi JS, Hong SY, Shin HW and Hong YK. 2001. Antifouling activity of seaweed extracts on the green alga Enteromorpha prolifera and the mussel Mytilus edulis, J App Phycol 13, 117-125. https://doi.org/10.1023/A:1011139910212
  9. Choi I, Yang YI, Song HD, Lee JS, Kang T, Sung J-J and Yi J. 2011.Lipid molecules induce the cytotoxic aggregation of Cu/Zn superoxide dismutase with structurally disordered regions. Bioch Bioph Acta 1812, 41-48.
  10. Dafforn KA, Lewis JA and Johnston EL. 2011. Antifouling strategies: History and regulation, ecological impacts and mitigation. Marine Pollution Bulletin 62, 453-465 https://doi.org/10.1016/j.marpolbul.2011.01.012
  11. Eckman CJE, Thistle D, Burnett WC, Patersin GLJ, Robertson CY and Lambshead PJD. 2001. Performance of cages as large animal-exclusion devices in the deep sea. J Mar Res 59, 79-95. https://doi.org/10.1357/002224001321237371
  12. Enright C. 1993. Control of fouling in bivalve aquaculture. World Aquac 24, 44-46.
  13. Fernandez-Alba AR, Hernando MD, Piedra L and Chisti Y. 2002. Toxicity evaluation of single and mixed antifouling biocides measured with acute toxicity bioassays. Anal Chim Acta. 456, 303-312. https://doi.org/10.1016/S0003-2670(02)00037-5
  14. Folke C, Kautsky N and Troell M. 1994. The costs of Eutrophication from salmon farming-implications for policy. J Environ Manag 40, 173-182. https://doi.org/10.1006/jema.1994.1013
  15. Fukuzawa A, Kumagai Y, Masamune T, Furusaki A, Katayama C and Matsumoto T. 1981. Acetylpinnasterol and pinnasterol, ecdysone-like metabolites from the marine red alga Laurencia pinnata Yamada. Tetrahedron Lett 22, 4085-4086. https://doi.org/10.1016/S0040-4039(01)82071-2
  16. Harold R, and Stanier RY. 1955. The genera Leucothrix and Thiothrix. Bacteriol Rev 19, 49-58.
  17. Haygood MG, Schmidt EW, Davidson SK and Faulkner DJ. 1999. Microbial symbionts of marine invertebrates: opportunities for microbial biotechnology. J Molec Microbiol. Biotechnol 1, 33-43.
  18. Heo HJ, Park YJ, Suh YM and Choi SJ. 2003. Effects of Oleamide on cholineacetyl transferase and cognitive activities. Biosci Biotechnol Biochem 67, 1284-1291 https://doi.org/10.1271/bbb.67.1284
  19. Lafont R and Mathieu M. 2007. Steroids in aquatic invertebrates. Ecotoxicology 16, 109-130. https://doi.org/10.1007/s10646-006-0113-1
  20. Myllymäki MJ. 2009. Synthesis of 3-heterocycle phenyl Nalkyl carbamates and their activity as FAAH inhibitors. Ph. D. Thesis, University of Technology, Helsinki, Finland
  21. Park SW, Lee JH and Park SW. 2009. Seaweed Disease. In:Disease of Laver. Bioscience Ltd, Seoul, Korea. 30-31.
  22. Qi SH, Miao L, Gao CH, Xu Y, Zhang S and Qian PY. 2010. New steroids and a new alkaloid from the gorgonian Isis minorbrachyblasta: structures, cytotoxicity, and antilarval activity. Helv Chim Acta 93, 511-516. https://doi.org/10.1002/hlca.200900250
  23. Qiu Y, Deng ZW, Xu M, Li Q and Lin WH. 2008. New A-nor steroids and their antifouling activity from the Chinese marine sponge Acanthella cavernosa. Steroids 73, 1500- 1504. https://doi.org/10.1016/j.steroids.2008.08.006
  24. Voulvoulis N. 2006. Antifouling paint booster biocides: occurrence and partitioning inwater and sediments. Env Chem 5, 155-170.
  25. Voulvoulis N, Scrimshaw MD and Lester JN. 2000. Occurrence of four biocides utilized in antifouling paints, as alternatives to organotin compounds, in waters and sediments of a commercial estuary in the UK. Marine Pollution Bulletin 40, 938-946. https://doi.org/10.1016/S0025-326X(00)00034-5
  26. Voulvoulis N, Scrimshaw MD and Lester JN. 1999. Alternative antifouling biocides. Appl. Organometal Chem 13, 135-143. https://doi.org/10.1002/(SICI)1099-0739(199903)13:3<135::AID-AOC831>3.0.CO;2-G
  27. Yamada H. 2007. Behaviour, occurrence, and aquatic toxicity of new antifouling biocides and preliminary assessment of risk to aquatic ecosystems. Bull Fish Res Agen 21, 31- 45.
  28. Yoshikawa K, Kanekuni S, Hanahusa M, Arihara S and Ohta T. 2000. Polyhydroxylated sterols from the octocoral Dendronephthya gigantea. J Nat Prod 63, 670-672. https://doi.org/10.1021/np990512v
  29. Zipser B, Bradford JJ and Hollingsworth RI. 1998. Cholesterol and its derivatives, are the principal steroids isolated from the leech species Hirudo medicinalis. Comp Biochem Physiol C 120, 269-282. https://doi.org/10.1016/S1095-6433(98)10029-6