Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Determination of Coccidiostats (Amprolium and Decoquinate) in Cattle and Chicken's Muscle using High Performance Liquid Chromatography

  • Received : 2011.05.12
  • Accepted : 2011.12.13
  • Published : 2012.02.20
Download PDF
⟨ Previous Next ⟩

Abstract

An analytical method for the simultaneous determination of veterinary medicines (amprolium and decoquinate) in cattle and chicken's muscle by HPLC/UV-vis was established. Samples were extracted by a HLB (Hydrophilic-Liphophilic Balance) cartridge with acetonitrile and methanol. Prior to HPLC injection, a mixture solvent (Water:MeOH, 1:1) was utilized as a reconstitution solvent. Chromatographic separation was achieved with a C18 column ($250{\times}4.6mm$, $5{\mu}m$) using gradient elution with 20 mM HFBA and MeOH:ACN (1:1.8). The calibration curves from the spiked blank matrix showed good linearity (above $r^2$=0.997) in the concentration range of $0.13-12.0mg\;kg^{-1}$. The relative recovery (accuracy) and limit of quantitation (LOQ) were in the range of 78.5-107.1% and $0.13-0.42mg\;kg^{-1}$, respectively. The developed method can be used to determine under the MRL (Maximum Residue Limits) levels of veterinary medicines in animal tissues.

Keywords

References

  1. Squadrone, S.; Mauro, C.; Ferro, G. L.; Amato, G.; Abete, M. C. J. Pharm. Biomed. Anal. 2008, 48, 1457. https://doi.org/10.1016/j.jpba.2008.09.024
  2. Olejnik, M.; Szprengier-Juszkiewicz, T.; Jedziniak, P. J. Chromatogr. A 2009, 1216, 8141. https://doi.org/10.1016/j.chroma.2009.04.097
  3. Peters, J. E.; Derijcke, J.; Verlinden, M.; Wyffels, R. Avian Dis. 1994, 38, 483. https://doi.org/10.2307/1592069
  4. Sanchez, A. A.; Campbell, H. M. J. AOAC Int. 2008, 91, 685.
  5. Reig, M.; Toldra, F. Meat Sci. 2008, 78, 60. https://doi.org/10.1016/j.meatsci.2007.07.029
  6. Girardi, C.; Odore, R. Vet. Res. Commun. 2008, 32, S11. https://doi.org/10.1007/s11259-008-9083-5
  7. Tan, H. S. I.; Ramachandran, P.; Caccini, W. J. Pharm. Biomed. Anal. 1996, 15, 259. https://doi.org/10.1016/0731-7085(96)01829-8
  8. Song, W.; Huang, M.; Rombeiha, W.; Li, H. Rapid Commun. Mass Spectrom. 2007, 21, 1944. https://doi.org/10.1002/rcm.3042
  9. Hamanoto, K.; Koike, R.; Shirakura, A.; Sasaki, N.; Machida, Y. J. Chromatogr. B 1997, 693, 489. https://doi.org/10.1016/S0378-4347(97)00088-1
  10. Furusawa, N. J. Chromatogr. Sci. 2002, 40, 355. https://doi.org/10.1007/BF02290372
  11. Commission Directive 1999/27/EC, 1999.

Cited by

  1. Simultaneous Determination of Five Coccidiostats in Veterinary Powders, Feed Premixes, and Baby Food by Micellar Electrokinetic Chromatography: Application to Chicken Tissues and Liver pp.1936-976X, 2018, https://doi.org/10.1007/s12161-018-1330-y
  2. Determination of 20 coccidiostats in milk, duck muscle and non-avian muscle tissue using UHPLC-MS/MS vol.30, pp.6, 2013, https://doi.org/10.1080/19440049.2013.794306
  3. Multi-residue analysis using liquid chromatography tandem mass spectrometry for detection of 20 coccidiostats in poultry, livestock, and aquatic tissues vol.27, pp.3, 2012, https://doi.org/10.1016/j.jfda.2019.02.004
  4. Determination of Eight Coccidiostats in Eggs by Liquid–Liquid Extraction–Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry vol.25, pp.4, 2012, https://doi.org/10.3390/molecules25040987