Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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A New Chemiluminescence Method for Determination of Cytosine Arabinoside in Pharmaceutical Preparations

  • Cai, Z. (College of Chemistry and Chemical Engineering, Guangxi University) ;
  • Zhang, X. (College of Chemistry and Chemical Engineering, Guangxi University) ;
  • Lu, D.F. (College of Chemistry and Chemical Engineering, Guangxi University) ;
  • Gan, J.N. (College of Chemistry and Chemical Engineering, Guangxi University)
  • Received : 2011.10.10
  • Accepted : 2011.11.17
  • Published : 2012.01.20
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Abstract

A novel chemiluminescence (CL) system was established for the determination of cytosine arabinoside (Ara-C) in pharmaceutical preparations. It was showed that a clear CL signal was observed when Eosin Y mixed with Fenton reagent. The CL intensity was decreased significantly when Ara-C was added to the reaction system and partially scavenged the hydroxyl radicals in the solution. The extent of decrease in the CL intensity had a good stoichiometrical relationship with the Ara-C concentration. Based on this, we developed a new method for the determination of Ara-C using a flow injection analysis (FIA) technique with CL detection. Under the optimal conditions, the linear range of Ara-C concentration was $6.0{\times}10^{-9}\sim1.0{\times}10^{-7}mol/L$ (R = 0.9982) with a detection limit of $7.6{\times}10^{-10}mol/L$ (S/N=3), the RSD was 5.6% for $6.0{\times}10^{-8}mol/L$ Ara-C (n = 11). The method was successfully applied to the determination of Ara-C in injection samples. The possible chemiluminescence reaction mechanism was discussed.

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References

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