Parasites, Hosts and Diseases

  • 제50권4호
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  • Pages.361-364
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  • 2012
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Short-Cut Pathway to Synthesize Cellulose of Encysting Acanthamoeba

  • Moon, Eun-Kyung (Department of Parasitology, Kyungpook National University School of Medicine) ;
  • Kong, Hyun-Hee (Department of Parasitology, Kyungpook National University School of Medicine)
  • 투고 : 2012.07.17
  • 심사 : 2012.11.02
  • 발행 : 2012.12.17
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초록

The mature cyst of Acanthamoeba is highly resistant to various antibiotics and therapeutic agents. Cyst wall of Acanthamoeba are composed of cellulose, acid-resistant proteins, lipids, and unidentified materials. Because cellulose is one of the primary components of the inner cyst wall, cellulose synthesis is essential to the process of cyst formation in Acanthamoeba. In this study, we hypothesized the key and short-step process in synthesis of cellulose from glycogen in encysting Acanthamoeba castellanii, and confirmed it by comparing the expression pattern of enzymes involving glycogenolysis and cellulose synthesis. The genes of 3 enzymes, glycogen phosphorylase, UDP-glucose pyrophosphorylase, and cellulose synthase, which are involved in the cellulose synthesis, were expressed high at the 1st and 2nd day of encystation. However, the phosphoglucomutase that facilitates the interconversion of glucose 1-phosphate and glucose 6-phosphate expressed low during encystation. This report identified the short-cut pathway of cellulose synthesis required for construction of the cyst wall during the encystation process in Acanthamoeba. This study provides important information to understand cyst wall formation in encysting Acanthamoeba.

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참고문헌

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피인용 문헌

  1. Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba vol.52, pp.2, 2012, https://doi.org/10.3347/kjp.2014.52.2.131
  2. Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes vol.164, pp.5, 2012, https://doi.org/10.1099/mic.0.000653
  3. The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba vol.13, pp.1, 2012, https://doi.org/10.1186/s13071-020-04237-5
  4. Mechanisms of Effector-Mediated Immunity Revealed by the Accidental Human Pathogen Legionella pneumophila vol.10, pp.None, 2012, https://doi.org/10.3389/fcimb.2020.593823
  5. Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes vol.10, pp.7, 2021, https://doi.org/10.3390/pathogens10070842