The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

The Protective Effects of Sasim-tang on the Brain Cell Damage in Photothrombotic Ischemia Mouse Model

광화학적 허혈성 뇌졸중 모델에서 사심탕(瀉心湯)의 뇌세포 손상 보호효과

  • Kang, Baek-Gyu (Dept. of Internal Medicine, College of Korean Medicine, Won-Kwang University) ;
  • Yun, Jong-Min (Dept. of Internal Medicine, College of Korean Medicine, Won-Kwang University) ;
  • Moon, Byung-Soon (Dept. of Internal Medicine, College of Korean Medicine, Won-Kwang University)
  • 강백규 (원광대학교 한의과대학 내과학교실) ;
  • 윤종민 (원광대학교 한의과대학 내과학교실) ;
  • 문병순 (원광대학교 한의과대학 내과학교실)
  • Published : 2012.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was designed to investigate the effects of Sasim-tang (SST) on proinflammatory cytokine production in a photothrombotic ischemia mouse model. Methods : Photothrombotic ischemia was induced in stereotactically held male Balb/c mice using rose bengal (10 mg/kg) and cold light. The target of photothrombotic ischemic lesion was 1 mm anterior to bregma and 3 mm lateral to midline with 2 mm in diameter, which are decreased by oral administration of SST. Results : SST protected ischemic death of brain cells through inhibition of pro-inflammatory cytokines production and catalytic activation of caspase-3 protease in photothrombotic ischemia mouse model. Conclusions : The results of this study suggest that SST can have protective effects on brain cell damage in a photothrombotic ischemia mouse model.

Keywords

References

  1. Drinagl U, Ladecola C, Moskowitz MA. Pathobiology of ischemic stroke ; An integrated view. Trends Neurosci 1999;22(9):391-7. https://doi.org/10.1016/S0166-2236(99)01401-0
  2. 서울대학교 의과대학 방사선학교실. 신경방사선과학. 서울: 일조각; 2004, p. 153.
  3. Narayaman U, Chi OZ, Liu X, Weiss HR. Effect of AMPA on cerebral cortical cxygen balance of ischemic rat brain. Neurochem Res 2000;25(3):405-11. https://doi.org/10.1023/A:1007505507532
  4. Pieper AA, Verma A, Zhang J. Snyder SH. Poly(ADP-ribose) polymerase, nitric oxide and cell death. Trends Pharmacol Sci 1999;20(4) :171-81. https://doi.org/10.1016/S0165-6147(99)01292-4
  5. 방오영. 허혈성 뇌졸중의 원인 및 발병기전. 대한뇌졸중학회지 2009;11(2):47-52.
  6. Clark WM. Cytokines and reperfusion injury. Neurology 1977;49(4):10-4.
  7. 張仲景. 金匱要略(中國醫學大系II). 서울: 驪江 出版社; 1986, p. 43.
  8. 譚日强. 金櫃要略淺述. 北京:人民衛生出版社; 1981, p. 310-2.
  9. 高山宏世 原著(趙蘊坤, 長文劍, 殷苦寒 編譯). 中醫方劑病症圖解. 山西: 山西科學技術出版社; 1991, p. 84-5.
  10. Donnan GA, Fisher M, Macleod M, Davis SM. Stroke. Lancet 2008;371(9624):1612-23. https://doi.org/10.1016/S0140-6736(08)60694-7
  11. 대한신경외과학회. 신경외과학. 서울: 중앙기획사; 2002, p. 263.
  12. 전국한의과대학심계내과학교실. 심계내과학. 서울: 군자출판사; 2006, p. 331-42, 393-408.
  13. 신민교. 임상본초학. 서울: 영림사; 1997, p. 400-1, 402-3, 785-6.
  14. 吳晋. 神農本草經. 서울: 醫道韓國社; 1976, p. (I 卷):12, (II卷):12, (III卷):7.
  15. 李時珍. 本草綱目. 台北: 文友書局; 1960, p. 447.
  16. 김형균. 한약의 약리. 서울: 고려의학; 2000, p. 211, 329, 333.
  17. 윤용갑. 동의방제와 처방해설. 서울: 의성당; 1998, p. 537.
  18. Schroeter M, Jander S, Stoll G. Non-invasive induction of focal cerebral ischemia in mice by photothrombosis of cortical microvessels. characterization of inflammatory responses. J Neurosci Methods 2002;117:43-9. https://doi.org/10.1016/S0165-0270(02)00072-9
  19. Watson BD, Dietrich WD, Busto R, Wachtel MS, Ginsberg MD. Induction of reproducible brain infarction by photochemically initiated thrombosis. Ann Neurol 1985;17(5):497-504. https://doi.org/10.1002/ana.410170513
  20. Searle J, Kerr JF, Bishop CJ. Necrosis and apoptosis: distinct modes of cell death with fundamentally different significance. Pathol Annu 1982;17:229-59.
  21. Solary E, Droin N, Bettaieb A, Corcos L, Dimanche-Boitrel MT, Garrido C. Positive and negative regulation of apoptotic pathways by cytotoxic agents in hematological malignancies. Leukemia 2000;14:1833-49. https://doi.org/10.1038/sj.leu.2401902
  22. Cohen JJ. Apoptosis: the physiologic pathway of cell death. Hosp Pract. 1993;28:35-43.
  23. 김광혁. 세포분자 면역학. 서울: 정문각; 1998, p. 34, 297.
  24. Halliwell B, Gutteridge JMC. Protection against oxidants in biological systems: the superoxide theory of oxygen toxicity. In Free radicals in biology and medicine. 2nd Ed. Oxford: Clarendon Press; 1999, p. 86-9.