Mycobiology

The Korean Society of Mycology (한국균학회)
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Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green

  • Son, Yu-Lim (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Kim, Hyoun-Young (Institute of Sunchang Fermented Soybean Products) ;
  • Thiyagarajan, Saravanakumar (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Xu, Jing Jing (Institute of Sunchang Fermented Soybean Products) ;
  • Park, Seung-Moon (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
  • Received : 2012.09.28
  • Accepted : 2012.10.14
  • Published : 2012.12.31
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Abstract

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces $H_2O_2$ over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of $H_2O_2$ improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to $150{\mu}M$ within 90 min.

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References

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