The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Effects of YideungJetong-Tang on Peripheral Neuropathy Induced by Taxol and Compression Injury in the Rat Sciatic Nerve

이등제통탕(二藤除痛湯)이 Taxol 처리 및 좌골신경의 압박 손상 후 유발된 랫드의 말초신경병증에 미치는 영향

  • Jeong, Ho Young (Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Kim, Chul Jung (Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Cho, Chung Sik (Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University)
  • 정호영 (대전대학교 부속 천안한방병원 신계내과학교실) ;
  • 김철중 (대전대학교 부속 천안한방병원 신계내과학교실) ;
  • 조충식 (대전대학교 부속 천안한방병원 신계내과학교실)
  • Received : 2012.06.01
  • Accepted : 2012.08.14
  • Published : 2012.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Most antitumor agents have the side effect of chemotherapy-induced peripheral neuropathy (CIPN). Cancer patients who take antitumor agents suffer from CIPN, but there is no known treatment for it. Unlike the central nerve system, the peripheral nerve can self-repair, and the Schwann cell takes this mechanism. Objectives: In this study, we researched the effect of YideungJetong-Tang (YJT) extract on taxol-induced sciatic nerve damage, through in vitro and in vivo experiments. Also, we studied the effect of YJT extract on neurite recovery and anti-inflammatory effect after compression injury of sciatic nerve in vivo. Methods: Vehicle, taxol and taxol+YJT were respectively applied on sciatic nerve cells of rat in vitro, then the cells were cultured. The sciatic nerve cells and Schwann cells were then observed using Neurofilament 200, Hoechst, ${\beta}$ -tubulin, S-$100{\beta}$, caspase-3 and phospho-Erk1/2. CIPN was induced by taxol into the sciatic nerve of rat in vivo, then YJT extract was taken orally. The axons, Schwann cells and neurites of the DRG sensory nerve were then observed using Neurofilament 200, ${\beta}$-tubulin, Hoechst, S-$100{\beta}$, phospho-Erk1/2 and caspase-3. YJT was taken orally after sciatic nerve compression injury, and the changes in axon of the sciatic nerve, Schwann cells and TNF-${\alpha}$ concentration were observed. Results: The taxol and YJT treated group showed significant effects on Schwann cell recovery, neurite growth and recovery. In vivo, YJT compared with control group showed Schwann cell structural improvement and axons recovering effect after taxol-induced Schwann cell damage. After sciatic nerve compression injury, recovery of distal axon, changes of Schwann cell distribution, and anti-inflammatory response were observed in the YJT. Conclusions: Through this study, we found that after taxol-induced neurite damage of sciatic nerve in vivo and in vitro, YJT had significant effects on sciatic nerve growth and Schwann cell structural improvement. In vivo, YJT improved recovery of distal axons and Schwann cells and had an anti-inflammatory effect.

Keywords

References

  1. Scholz J, Broom DC, Youn DH, Mills CD, Kohno T, Suter MR, et al. Blocking caspase activity prevents transsynaptic neuronal apoptosis and the loss of inhibition in lamina II of the dorsal horn after peripheral nerve injury. J Neurosci. 2005;25(32):7317-23. https://doi.org/10.1523/JNEUROSCI.1526-05.2005
  2. Fu SY, Gordon T. The cellular and molecular basis of peripheral nerve regeneration. Mol Neurobiol. 1997;14(1-2):67-116. https://doi.org/10.1007/BF02740621
  3. Dennis K, Eugene B, Anthony F, Stephen H, Dan LJJ, et al. HARRISON'S 내과학. 17th. 서울:MIP. 2010:3185-7.
  4. Rowinsky EK, Eisenhauer EA, Chaudhry V, Arbuck SG, Donehower RC. Clinical toxicities encountered with paclitaxel (Taxol). Semin Oncol. 1993;20(4 Suppl 3):1-15.
  5. Carozzi VA, Canta A, Oggioni N, Sala B, Chiorazzi A, Meregalli C, et al. Neurophysiological and neuropathological characterization of new murine models of chemotherapy-induced chronic peripheral neuropathies. Exp Neurol. 2010;226(2):301-9. https://doi.org/10.1016/j.expneurol.2010.09.004
  6. Wickham R. Chemotherapy-induced peripheral neuropathy: a review and implications for oncology nursing practice. Clin J Oncol Nurs. 2007; 11(3):361-76. https://doi.org/10.1188/07.CJON.361-376
  7. Kuroi K, Shimozuma K, Ohashi Y, Takeuchi A, Aranishi T, Morita S, et al. A questionnaire survey of physicians' perspectives regarding the assessment of chemotherapy-induced peripheral neuropathy in patients with breast cancer. Jpn J Clin Oncol. 2008;38(11):748-54. https://doi.org/10.1093/jjco/hyn100
  8. Donovan D. Management of peripheral neuropathy caused by microtubule inhibitors. Clin J Oncol Nurs. 2009;13(6):686-94. https://doi.org/10.1188/09.CJON.686-694
  9. Windebank AJ, Grisold W. Chemotherapy-induced neuropathy. J Peripher Nerv Syst. 2008; 13(1):27-46. https://doi.org/10.1111/j.1529-8027.2008.00156.x
  10. Armstrong T, Almadrones L, Gilbert MR. Chemotherapy-induced peripheral neuropathy. Oncol Nurs Forum 2005;32(2):305-11. https://doi.org/10.1188/05.ONF.305-311
  11. 전국한의과대학심계내과학교실. 심계내과학. 서울:군자출판사. 2006:343-9.
  12. Kim JM, Cho CS, KIM CJ. Clinical Study of 8 Diabetic Patients with Paresthesia. Korean J. Orient.Int. Med. 2010;31(2):184-91.
  13. Cho JH, Kim JM, Kim JH, Oh YS, Kim CJ. A Case Report of Chemotherapy-induced Neuropathic Pain Treated with Oriental Medicine. The Journal of Korean Oriental Medicine 2010;31(6):38-62.
  14. Banker G, Goslin K. Culturing nerve cells. MIT press. 2002.
  15. 아담스 신경과학 편찬위원회. PRINCIPLE OF NEUROLOGY 신경과학. 서울:정담. 1998:827, 833, 1061, 1157-9, 1202, 1204.
  16. 이태희. 임상 신경학 총론. 서울: 고려의학. 1999:136.
  17. Postma TJ, Aaronson NK, Heimans JJ, Muller MJ, Hildebrand JG, Delattre JY, et al. EORTC Quality of Life Group. The development of an EORTC quality of life questionnaire to assess chemotherapy- induced peripheral neuropathy: the QLQ-CIPN20. Eur J Cancer 2005;41(8):1135-9. https://doi.org/10.1016/j.ejca.2005.02.012
  18. Kwak MK, Kim EJ, Lee ER, Kwan IG, Hwang MS. Characteristics and Quality of Life in Patients with Chemotherapy-Induced Peripheral Neuropathy. J Korean Oncol Nurs. 2010;10(2): 231-9. https://doi.org/10.5388/jkon.2010.10.2.231
  19. Chaudhry V, Rowinsky EK, Sartorius SE, Donehower RC, Cornblath DR. Peripheral neuropathy from taxol and cisplatin combination chemotherapy: clinical and electro physiological studies. Ann Neurol. 1994;35(3):304-11. https://doi.org/10.1002/ana.410350310
  20. Forsyth PA, Balmaceda C, Peterson K, Seidman AD, Brasher P, DeAngelis LM. Prospective study of paclitaxel-induced peripheral neuropathy with quantitative sensory testing. J Neurooncol. 1997;35(1):47-53. https://doi.org/10.1023/A:1005805907311
  21. Backonja M, Beydoun A, Edwards KR, Schwartz SL, Fonseca V, Hes M, et al. Gabapentin for the symptomatic treatment of painful neuropathy in patients with diabetes mellitus: a randomized controlled trial. JAMA. 1998;280(21):1831-6. https://doi.org/10.1001/jama.280.21.1831
  22. Rao RD, Michalak JC, Sloan JA, Loprinzi CL, Soori GS, Nikcevich DA, et al. North Central Cancer Treatment Group. Efficacy of gabapentin in the management of chemotherapy-induced peripheral neuropathy: a phase 3 randomized, double- blind, placebo-controlled, crossover trial (N00C3). Cancer. 2007;110(9):2110-8. https://doi.org/10.1002/cncr.23008
  23. Kautio AL, Haanpää M, Leminen A, Kalso E, Kautiainen H, Saarto T. Amitriptyline in the prevention of chemotherapy-induced neuropathic symptoms. Anticancer Res. 2009;29(7):2601-6.
  24. Scripture CD, Figg WD, Sparreboom A. Peripheral neuropathy induced by paclitaxel: recent insights and future perspectives. Curr Neuropharmacol. 2006;4(2):165-72. https://doi.org/10.2174/157015906776359568
  25. Flatters SJ, Bennett GJ. Studies of peripheral sensory nerves in paclitaxel-induced painful peripheral neuropathy: evidence for mitochondrial dysfunction. Pain. 2006;122(3):245-57. https://doi.org/10.1016/j.pain.2006.01.037
  26. Stoll G, Müller HW. Nerve injury, axonal degeneration and neural regeneration: basic insights. Brain Pathol. 1999;9(2):313-25.
  27. David S, Aguayo AJ. Axonal regeneration after crush injury of rat central nervous system fibres innervating peripheral nerve grafts. J Neurocytol. 1985;14(1):1-12. https://doi.org/10.1007/BF01150259
  28. Fawcett JW. Peripheral nerve regeneration in Brain damage, Brain repair, edited by JW Fawcett, AE Rosser, SB Dunnett. Oxford University Press (NY). 2001:143-45.
  29. Norbury C, Nurse P. Animal cell cycles and their control. Annu Rev Biochem. 1992;61:441-70. https://doi.org/10.1146/annurev.bi.61.070192.002301
  30. Han IS, Seo TB, Kim KH, Yoon JH, Yoon SJ, Namgung U. Cdc2-mediated Schwanncell migration during peripheral nerve regeneration. J Cell Sci. 2007;120(Pt 2):246-55. https://doi.org/10.1242/jcs.03322
  31. 전국한의과대학 본초학 편찬위원회. 본초학. 서울:영림사. 2004:485, 537, 544.
  32. 이기태, 유병찬, 김윤식, 설인찬. 四物湯이 손상된 말초신경섬유 재생에 미치는 효과에 대한 사전 연구. 대전대학교 한의학연구소 논문집 2005; 14(2):107-12.
  33. Lim SM, Ahn JJ, Jo HK, Yoo HR, Kim YS, Seol IC. Effect of Gyehyuldeung Treatments in Peripheral Nerve Regeneration of Rat. The Journal of Korean Oriental medicine 2009;30(2): 375-87.
  34. Tsang D, Yeung HW, Tso WW, Peck H. Ginseng saponins: influence on neurotransmitter uptake in rat brain synaptosomes. Planta Med. 1985;(3): 221-4.
  35. 鄭虎占, 董澤宏, 徐靖. 主編中藥現代硏究與應用. 北京:學苑出版社. 1998:2057-77.
  36. Jeong SH, Jeong SH, Shin GC, Lee WC. Protective Effect Puerariae Radix on Hypoxia Reperfusion induced by PC12 Cell Damage and Global Ischemia in Gerbil. Korean J. Orient.Int. Med. 2003;24(4):767-77.