Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Novel Marker for the Species-Specific Detection and Quantitation of Shigella sonnei by Targeting a Methylase Gene

  • Cho, Min Seok (National Academy of Agricultural Science, Rural Development Administration) ;
  • Ahn, Tae-Young (Department of Microbiology, Dankook University) ;
  • Joh, Kiseong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kwon, Oh-Sang (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research Environmental Research Complex) ;
  • Jheong, Won-Hwa (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research Environmental Research Complex) ;
  • Park, Dong Suk (National Academy of Agricultural Science, Rural Development Administration)
  • Received : 2011.11.07
  • Accepted : 2012.04.18
  • Published : 2012.08.28
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Abstract

Shigella sonnei is a causal agent of fever, nausea, stomach cramps, vomiting, and diarrheal disease. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection of S. sonnei using a primer pair based on the methylase gene for the amplification of a 325 bp DNA fragment. The qPCR primer set for the accurate diagnosis of Shigella sonnei was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.

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References

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