Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biosynthesis of Xylobiose: A Strategic Way to Enrich the Value of Oil Palm Empty Fruit Bunch Fiber

  • Lakshmi, G. Suvarna (Bioengineering and Environmental Centre, Indian Institute of Chemical Technology) ;
  • Rajeswari, B. Uma (Bioengineering and Environmental Centre, Indian Institute of Chemical Technology) ;
  • Prakasham, R.S. (Bioengineering and Environmental Centre, Indian Institute of Chemical Technology)
  • Received : 2012.02.03
  • Accepted : 2012.03.21
  • Published : 2012.08.28
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Abstract

Xylooligosaccharides are functional foods mainly produced during the hydrolysis of xylan by physical, chemical, or enzymatic methods. In this study, production of xylobiose was investigated using oil palm empty fruit bunch fiber (OPEFB) as a source material, by chemical and enzymatic methods. Xylanase-specific xylan hydrolysis followed by xylobiose production was observed. Among different xylanases, xylanase from FXY-1 released maximum xylobiose from pretreated OPEFB fiber, and this fungal strain was identified as Aspergillus terreus and subsequently deposited under the accession Number MTCC- 8661. The imperative role of lignin on xylooligosaccharides enzymatic synthesis was exemplified with the notice of xylobiose production only with delignified material. A maximum 262 mg of xylobiose was produced from 1.0 g of pretreated OPEFB fiber using FXY-1 xylanase (6,200 U/ml) at pH 6.0 and $45^{\circ}C$. At optimized environment, the yield of xylobiose was improved to 78.67 g/100 g (based on xylan in the pretreated OPEFB fiber).

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References

  1. Aachary, A. A. and S. G. Prapulla. 2009. Value addition to corncob: Production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154. Bioresour. Technol. 100: 991-995. https://doi.org/10.1016/j.biortech.2008.06.050
  2. Aachary, A. A. and S. G. Prapulla. 2008. Corncob-induced endo-1,4-${\beta}$-D-xylanase of Aspergillus oryzae MTCC 5154: Production and characterization of xylobiose from glucuronoxylan. J. Agric. Food Chem. 56: 3981-3988. https://doi.org/10.1021/jf073430i
  3. Ai, Z., Z. Jiang, L. Li, W. Deng, I. Kusakabe, and H. Li. 2005. Immobilization of Streptomyces olivaceoviridis E-86 xylanase on Eudragit S-100 for xylo-oligosaccharide production. Process Biochem. 40: 2707-2714. https://doi.org/10.1016/j.procbio.2004.12.006
  4. Akpinar, O., O. Ak, A. Kavas, U. Bakir, and L. Yilmaz. 2007. Enzymatic production of xylooligosaccharides from cotton stalks. J. Agric. Food Chem. 55: 5544-5551. https://doi.org/10.1021/jf063580d
  5. Akpinar, O., K. Erdogan, U. Bakir, and L. Yilmaz. 2010. Comparison of acid and enzymatic hydrolysis of tobacco stalks xylan for preparation of xylooligosaccharides. LWT Food Sci. Technol. 43: 19-25.
  6. Alonso, J. L., H. Dominguez, G. Garrote, J. C. Parajo, and M. J. Vazquez. 2003. Xylooligosaccharides: Properties and production technologies. Elec. J. Env. Agricult. Food Chem. 2: 230-232.
  7. Bailey, M. J., P. Biely, and K. Poutanen. 1992. Inter-laboratory testing of methods for assay of xylanase activity. J. Biotechnol. 23: 257-270. https://doi.org/10.1016/0168-1656(92)90074-J
  8. Beg, Q. K., M. Kapoor, L. Mahajan, and G. S. Hoondal. 2011. Microbial xylanases and their industrial applications: A review. Appl. Microbiol. Biotechnol. 56: 326-338.
  9. Brienzo, M., W. Carvalho, and A. M. F. Milagres. 2010. Xylooligosaccharides production from alkali-pretreated sugarcane bagasse using xylanases from Thermoascus aurantiacus. Appl. Biochem. Biotechnol. 162: 1195-1205. https://doi.org/10.1007/s12010-009-8892-5
  10. Chapla, D., P. Pandit, and A. Shah. 2011. Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics. Bioresour Technol. [In Press].
  11. Mazzaferro, L. S., M. M. Cuna, and J. D. Breccia. 2011. Production of xylo-oligosaccharides by chemo-enzymatic treatment of agricultural by-products. BioResources 6: 5050-5061.
  12. Nabarlatz, D., X. Farriol, and D. Montane. 2005. Auto hydrolysis of almond shells for the production of xylo-oligosaccharides: Product characteristics and reaction kinetics. Ind. Eng. Chem. Res. 44: 7746-7755. https://doi.org/10.1021/ie050664n
  13. Rahman, S. H. A., J. P. Choudhury, and A. L. Ahmad. 2006. Production of xylose from oil palm empty fruit bunch fiber using sulphuric acid. Biochem. Eng. J. 30: 97-103. https://doi.org/10.1016/j.bej.2006.02.009
  14. Sabiha-Hanim, S., M. A. M. Noor, and A. Rosma. 2011. Effect of auto hydrolysis and enzymatic treatment on oil palm (Elaeis guineensis Jacq.) frond fibres for xylose and xylooligosaccharides production. Bioresour. Technol. 102: 1234-1239. https://doi.org/10.1016/j.biortech.2010.08.017
  15. Suvarna Lakshmi, G., Ch. Subba Rao, R. Sreenivasa Rao, P. J. Hobbs, and R. S. Prakasham. 2009. Enhanced production of xylanase by a newly isolated Aspergillus terreus under solid state fermentation using palm industrial waste: A statistical optimization. Biochem. Eng. J. 48: 51-57. https://doi.org/10.1016/j.bej.2009.08.005
  16. Teng, C., Q. Yan, Z. Jiang, G. Fan, and B. Shi. 2010. Production of xylooligosaccharides from the steam explosion liquor of corncobs coupled with enzymatic hydrolysis using a thermo stable xylanase. Bioresour. Technol. 101: 7679-7682. https://doi.org/10.1016/j.biortech.2010.05.004
  17. Voragen, A. G. J. 1998. Technological aspects of functional food related carbohydrates. Trends Food Sci. Technol. 9: 328-335. https://doi.org/10.1016/S0924-2244(98)00059-4
  18. Yang, H., K. Wang, X. Song, and F. Xu. 2011. Production of xylooligosaccharides by xylanase from Pichia stipitis based on xylan preparation from triploid Populas tomentosa. Bioresour. Technol. 102: 7171-7176. https://doi.org/10.1016/j.biortech.2011.03.110
  19. Yang, R., S. Xu, Z. Wang, and W. Yang. 2005. Aqueous extraction of corncob xylan and production of xylooligosaccharides. LWT Food Sci. Technol. 38: 677-682. https://doi.org/10.1016/j.lwt.2004.07.023

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