한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제40권3호
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  • Pages.190-196
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  • 2012
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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미생물제제시용 고추경작지로부터 식물생장홀몬과 항진균물질을 동시에 생산하는 식물생장촉진근권세균의 선발 및 동정

Selection and Identification of Phytohormones and Antifungal Substances Simultaneously Producing Plant Growth Promoting Rhizobacteria from Microbial Agent Treated Red-pepper Fields

  • 정병권 (영남대학교 미생물생명공학과) ;
  • 임종희 (영남대학교 미생물생명공학과) ;
  • 안창환 (영남대학교 미생물생명공학과) ;
  • 김요환 (영남대학교 미생물생명공학과) ;
  • 김상달 (영남대학교 미생물생명공학과)
  • Jung, Byung-Kwon (Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University) ;
  • Lim, Jong-Hui (Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University) ;
  • An, Chang-Hwan (Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University) ;
  • Kim, Yo-Hwan (Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University) ;
  • Kim, Sang-Dal (Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University)
  • 투고 : 2012.07.03
  • 심사 : 2012.09.06
  • 발행 : 2012.09.28
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초록

식물생장 촉진 홀몬 auxin을 생산하는 균주를 선발하기 위해 경북 경산시 소재 고추경작지 근권토양으로 부터 739종의 일반호기성 균주와 urease 생산 균주 80종 및 광합성 균주 303종과 같이 총 1000여종 이상의 균주를 분리하였다. 이 균주들을 대상으로 Salkowski test를 실시한 결과, auxin을 생산하는 158종의 일반호기성 균주와 70종의 urease 생산 균주 및 228종의 광합성 균주를 선발할 수 있었으며, Holbrook test를 통해 또 다른 식물생장 촉진 호르몬인 gibberellin도 대부분의 균주에서 생산되는 것을 확인할 수 있었다. 선발된 균주 중 항진균 물질인 ${\beta}$-Glucanase와 siderophore를 생산하고 다양한 병원성 진균에 대해 길항 범위를 가지는 6가지 균주 BCB14, BCB17, C10, HA46, HA143, HJ5를 toothpicking 및 대치배양을 통해 최종 선발할 수 있었으며, 분류학적으로 동정한 결과 6종 모두 B. subtilis BCB14, B. methylotrophicus BCB17, B. methylotrophicus C10, B. sonorensis HA46, B. subtilis HA143, B. safensis HJ5로 확인되었다.

In this study, a total of more than 1,000 bacteria, including 739 species of aerobic bacteria, 80 species of urease producing bacteria and 303 species of photosynthetic bacteria, were isolated from red-pepper field soils located in the Gyeongsan Province of the Republic of Korea. Amongst these, 158 species of aerobic bacteria, 70 species of urease producing bacteria and 228 species of photosynthetic bacteria were found to be auxin producing soil bacteria through quantification analysis with the Salkowski test. The latter groupings were then tested for antifungal activities to ${\beta}$-Glucanase and siderophore using CMC congo red agar and CAS blue agar media. In addition, the selected strains were examined for antifungal activity against various phytopathogenic fungi on PDN agar media. Six strains; BCB14, BCB17, C10, HA46, HA143, and HJ5, were noted for their ability to both produce auxin and act as antifungal substances. 16S rDNA sequence comparison analyses of these six strains identified them as Bacillus subtilis BCB14, B. methylotrophicus BCB17, B. methylotrophicus C10, B. sonorensis HA46, B. subtilis HA143, and B. safensis HJ5.

키워드

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  2. 토양에서 분리한 Pseudomonas geniculata ANG3, Exiguobacterium acetylicum ANG40 및 Burkholderia stabilis ANG51의 식물 생장촉진 활성 및 식물병 방제활성 vol.48, pp.1, 2012, https://doi.org/10.4014/mbl.1906.06002