KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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In vivo Dendritic Cell Migration Tracking Using Near-infrared (NIR) Imaging

Near-infrared (NIR) 영상기법을 이용한 생체 내 수지상세포의 이동

  • Lee, Jun-Ho (Department of Applied Bioscience, CHA University) ;
  • Jung, Nam-Chul (Department of Applied Bioscience, CHA University) ;
  • Lee, Eun Gae (Department of Applied Bioscience, CHA University) ;
  • Lim, Dae-Seog (Department of Applied Bioscience, CHA University)
  • 이준호 (차의과학대학교 바이오산업응용학과) ;
  • 정남철 (차의과학대학교 바이오산업응용학과) ;
  • 이은계 (차의과학대학교 바이오산업응용학과) ;
  • 임대석 (차의과학대학교 바이오산업응용학과)
  • Received : 2012.09.14
  • Accepted : 2012.10.24
  • Published : 2012.10.31
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Abstract

Matured dendritic cells (DCs) begin migration with their release from the bone marrow (BM) into the blood and subsequent traffic into peripheral lymphoid and non-lymphoid tissues. Throughout this long movement, migrating DCs must apply specialized skills to reach their target destination. Non-invasive in vivo cell-tracking techniques are necessary to advance immune cell-based therapies. In this study, we used a DiD cell-tracking solution for in vivo dendritic cell tracking in naive mice. We tracked DiD (non-invasive fluorescence dye)-labeled mature dendritic cells using the Near Infrared (NIR) imaging system in normal mice. We examined the immunophenotype of DiD-labeled cells compared with non-labelled mature DCs, and obtained time-serial images of NIR-DC trafficking after mouse footpad injection. In conclusion, we confirmed that DiD-labeled DCs migrated into the popliteal lymph node 24 h after the footpad injection. Here, these data suggested that the cell tracking system with the stable fluorescence dye DiD was useful as a cell tracking tool to advance dendritic cell-based immunotherapy.

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