• Title/Summary/Keyword: NIR

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The Quantification and Validation of Loxoprofen using Near-infrared(NIR) Spectrum Method (근적외부스펙트럼 측정법을 이용한 록소프로펜의 정량화 및 밸리데이션)

  • Choi, Sung-Up
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.1
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    • pp.396-401
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    • 2014
  • In this study, we used NIR spectrum method instead of conventional HPLC method to shorten the analysis and manufacturing time of the loxoprofen products. Loxoprofen mixtures with other pharmaceutical additives were prepared and evaluated by the NIR spectrometer and the HPLC system. Validation of both methods was performed for specificity, accuracy and precision. NIR spectrometer method was validated and revealed proper results for the in-process quality control in the pharmaceutical field. In conclusion, NIR spectrometry can be replaced by HPLC method.

Characterization of a Chromosomal Nickel Resistance Determinant from Klebsiella oxytoca CCUG 15788

  • Park, Jae-Sun;Lee, Sung-Jae;Rhie, Ho-Gun;Lee, Ho-Sa
    • Journal of Microbiology and Biotechnology
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    • v.18 no.6
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    • pp.1040-1043
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    • 2008
  • Klebsiella oxytoca CCUG 15788 is resistant to $Ni^{2+}$ at a concentration of 10 mM and grows in an inducible manner when exposed to lower concentrations of $Ni^{2+}$. The complete genomic sequence of a 4.2-kb HindIII-digested fragment of this strain was determined from genomic DNA. It was shown to contain four nickel resistance genes (nirA, nirB, nirC, and nirD) encoding transporter and transmembrane proteins for nickel resistance. When the plasmid pKOHI4, encoding nirABCD, was transformed into Escherichia coli JM109, the cells were able to grow in Tris-buffered mineral medium containing 3 mM nickel. TnphoA'-1 insertion mutants in the four nickel genes nirA, nirB, nirC, and nirD showed nickel sensitivity. The nir genes were heterogeneously expressed in E. coli, suggesting functional roles of these genes in nickel resistance.

Study on Improvement of Signal to Background Ratio of Laser-based Fluorescence Imaging System (레이저 기반 형광 영상 시스템의 Signal to Background Ratio 향상 연구)

  • Kim, J.H.;Jeong, M.Y.
    • Journal of the Microelectronics and Packaging Society
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    • v.27 no.4
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    • pp.107-111
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    • 2020
  • Recently, as an aging society progresses, a lot of interest in health and diagnosis is increasing, As the field of various bio-imaging systems for guided surgery capable of accurate diagnosis has emerged as important, a Fluorescence imaging system capable of accurate measurement and real-time confirmation has emerged as an important field. Fluorescence images currently being used are mainly in the NIR-I band, but many studies are in progress in the NIR-II band in order to improve resolution and confirm fluorescence deeply and accurately. In this paper, the difference between NIR-I and NIR-II, optical characteristics, and SBR (signal to background ration) of a fluorescent imaging system, was investigated using the finite element (FEM) method. After confirming, it was confirmed that the SBR was 16.2 times higher in the NIR-II area than in the NIR-I by making the skin phantom and measuring the fluorescence. It is confirmed that the enhancement in SBR of the Fluorescence imaging system is more effective in the NIR-II region than in the NIR-I region and expected to be used in application fields such as guided surgery, bio-sensor and also device which can detect the defect of optical devices.

Defect Detection of ‘Fuji’ Apple using NIR Imaging(I) -Optical characteristics of defects and selection of significant wavelelength- (근적외선 영상을 이용한 후지사과의 결점 검출에 관한 연구 (I) -결점의 광학적 특성 구명 및 유의파장 선정-)

  • 이수희;노상하
    • Journal of Biosystems Engineering
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    • v.26 no.2
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    • pp.169-176
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    • 2001
  • Defect of apple was depreciated the product value and causes storage disease seriously. To detect the defect of ‘Fuji’apple with machine vision system, the optical characteristics of defect should be investigated. In this research, absorbance spectra of defect were acquired by spectrophotometer in the range of visible and NIR region(400∼1,100nm) and L*a*b* color values were also acquired by colorimeter. NIR machine vision system was constructed with B&W camera, frame grabber, 16 tungsten-halogen lamps, variable focal length lens and NIR bandpass filter which was mounted to lens outward. Average gray values of defect at 15 NIR wavelength were acquired and the significant NIR wavelength was selected by comparing Mahalanobis distance between sound and defective apple. As the result of Mahalanobis distance analysis, the significant wavelength to discriminate the defectives in ‘Fuji’apple were found to be 720nm for scab and 970nm for bruise and cuts and 920nm was also effective regardless of defective types.

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HAND-HELD NIR SPECTROMETRY : STATUS, TRENDS AND FUTURISTIC PROSPECTS

  • McClure, W.F.
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1051-1051
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    • 2001
  • In 1989, at the 2nd ICNIRS Meeting in Tsukuba, I projected certain directions for NIR technology would take in the future. Among those projections were: (1) An inrush of companies producing FT-NIR instruments. (2) Hybrid NIR Systems (3) Hand-held NIR Technology. All three thrusts have resulted in numerous commercial offerings over the last 12 years Hand-held technology for all fields is growing at an astonishing rate. To date, NIR work at North Carolina State University has produced four (4) hand-held NIR units for: (1) Nicotine and Moisture in tobacco, (2) Vanillin and Moisture in Vanilla Beans, (3) Protein, Moisture and Nitrogen in plant tissue, (4) Chlorophyll and Moisture in Growing Plants: A NIR Spectrometer for Developing Countries. This paper will discuss these developments, including design and performance data.

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Synthesis and Characterization of Quaterrylene Bisimide as NIR Colorant (NIR Colorant용 Quaterrylene Bisimide의 합성 및 특성 연구)

  • Park, Keun-Soo;Jeong, Yeon-Tae
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.24 no.5
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    • pp.398-403
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    • 2011
  • Recently, Near-infrared (NIR)colorant is intriguing and attractive but full of challenges. Although some cyanine colorant have been commercialized, near-infrared colorant with intensive NIR absorption, good chemical and photo-stability, and high solubility still remain as target compound. Certain polycyclic aromatic compounds such as quaterrylene represent a key class of NIR colorant and also give rise to outstanding physical and chemical properties after appropriate chemical modification. In this study, We have tried to introduceimide functional group to quaterrylene in order to give chemical and thermal stability. Finally, N,N'-bis (2,6-diisopropylphenyl)-quarterrylene-3,4:13,14-tetracarboximide was synthesized and evaluated its properties using $^1H$ NMR, Maldi-tof, TGA, and UV/VIS/NIR spectroscopy as NIR colorant. The quaterrylene bisimide compound exhibit a excellent thermal stability and chemical stability.

Determination of water content in alcohol by portable near infrared (NIR) system (휴대용 분광분석기를 이용한 알코올 중에 함유되어 있는 물의 측정)

  • Ahn, Jhii-Weon;Woo, Young-Ah;Kim, Hyo-Jin
    • Analytical Science and Technology
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    • v.16 no.2
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    • pp.95-101
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    • 2003
  • In this study, water content in the mixture of methanol and ethanol was nondestructively measured by near infrared (NIR) spectroscopy. Two types of NIR instruments, portable NIR system with a photo-diode array and scanning type NIR spectrometer were used and the calibration results were compared. Partial least squares regression (PLSR) was applied for the calibration and validation for the quantitative analysis. The calibration results from both instruments showed good correlation with actual values. The calibration with the use of PLS model predicted water concentration with a standard error of prediction (SEP) of 0.10% and 0.12% for photo diode array and scanning type, respectively. During 6 days, routine analyses for 3%, 5% and 7% water in ethanol solution with 2% methanol were performed to validate the robustness of the developed calibration model. The routine analyses showed good results with coefficient of variation (CV) of within 3% for both types of NIR spectrometers. This study showed that the rapid determination of water in the mixture of methanol and ethanol was successfully performed by NIR spectroscopy and the performance of the portable NIR system with a photo diode array detector was comparable to that of the scanning type NIR spectrometer.

Quantification of Skin Moisture in Hairless Mouse by using a Portable NIR System and a FT NIR Spectrometer (Photo Diode Array형의 휴대용 근적외 분광기와 FT 근적외 분광기를 이용한 Hairless Mouse 피부 수분 정량)

  • Suh, Eun-Jung;Woo, Young-Ah;Kim, Hyo-Jin
    • YAKHAK HOEJI
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    • v.49 no.2
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    • pp.115-121
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    • 2005
  • In this study, the performance of a portable NIR system and a FT NIR spectrometer were compared to determine water content of hairless mouse skin. The stratum corneum parts wer e separated from the epidermal tissues by trypsin solution. NIR diffuse reflectance spectra of hairless mouse skin were acquired using a fiber optic probe. In the near infrared, water molecules show two clear absorption bands at 1450 nm from first overtone of O-H stretching and 1940 nm from the combination involving O-H stretching and O-H deformation. It was found that the variations of O-H absorption band according to water content. Partial least squares regression (PLSR) was applied to develop a calibration model. The PLS model showed a good correlation between NIR predicted value and the absolute water content of separated hairless mouse skin, in vitro. For both the portable and the FT NIR spectrometer, These studies showed the possibility of a rapid and nondestructive skin moisture measurement using NIR spectroscopy. The portable NIR spectrometer with a photodiode arrays-microsensor could be more rapidly applied for the determination of water content with comparable accuracy with the performance of a FT spectrometer .

Early Detection of Clear Egg in Incubation Using VIS/NIR Spectroscopy (VIS/NIR 분광분석법을 이용한 미부화란의 조기 검출)

  • Kim, Hak Sung;Kim, Ghi Seok;Kim, Yong Ro;Kang, Seok Won;Noh, Sang Ha
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2017.04a
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    • pp.104-104
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    • 2017
  • 정상적인 부화 여부를 판별하기 위한 1차 검란은 일반적으로 5일~7일 이후에 시행된다. 미부화란을 이보다 더 빠른 시간 안에 검출할 경우 부화에 소요되는 에너지의 감소 효과 및 미부화란을 다른 용도로 활용하는 것을 기대할 수 있다. 시중에서 쉽게 구입할 수 있는 산란계인 하이라인 브라운 품종의 유정란 29개와 인위적인 미부화란을 만들기 위한 동일 품종의 무정란 11개를 사용하였으며 $38^{\circ}C$, 70% 조건의 항온항습기에서 96시간 동안 부화하였다. 스펙트럼 획득 장치의 광원은 녹색영역을 발광하는 LED램프와 일반 할로겐 광원을 별도로 사용하였으며 스펙트로미터는 VIS/NIR 영역인 520~1,180nm영역과 NIR영역인 900~1,700nm영역의 것을 사용하였다. 부화 시작 전과 부화 시작 후 1일 간격으로 각각 1개의 샘플에 대한 1개의 스펙트럼을 측정하였다. 측정 영역은 LED광원을 이용한 경우는 520~1,1800nm, 할로겐광원을 이용한 경우에는 520~1,180nm와 900~1,700nm이었다. 정상 부화여부는 4일차에서 할란하여 확인하였고, 측정 일자별로 PLS-DA분석법을 이용한 판별 모델을 개발하였다. 4일차에서 유정란 29개 중 11개가 정상 부화하였고, 18개는 미부화하였다. 3일차에서 판별 모델의 정확도는 LED광원의 VIS/NIR 영역 스펙트럼을 이용한 경우는 100%, 할로겐 광원의 VIS/NIR 영역 스펙트럼을 이용한 경우는 70%, 할로겐 광원의 NIR영역 스펙트럼을 이용한 경우는 70%였다. 4일차에서 판별 모델의 정확도는 LED광원의 VIS/NIR 영역 스펙트럼을 이용한 경우는 100%, 할로겐 광원의 VIS/NIR 영역 스펙트럼을 이용한 경우는 90%, 할로겐 광원의 NIR영역 스펙트럼을 이용한 경우는 100%였다. 부화 3일차는 정상 부화할 경우 피가 생성되는 시기이다. 피가 형성된 이후의 부화 여부를 판단하는 광원으로는 할로겐램프보다 LED램프를 사용하는 것이 더 적합한 것으로 나타났다.

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The Study of Thermo-physiological Responses with Near Infrared Lighted Garment at a Hot Environment (서열환경에서 근적외선 조사의복 착용시의 온열생리반응)

  • Kim, Seong-Suk;Kim, Woo-Jong;Kim, Hee-Eun
    • Fashion & Textile Research Journal
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    • v.7 no.6
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    • pp.665-672
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    • 2005
  • The purpose of this study was to assess the feasibility and the effects of near infrared lighted garments on thermo-physiological responses in human body. Seven healthy adult men were recruited for this study. All subjects were informed the contents and purpose of this study. The experiment was carried out in a climate chamber of $32^{\circ}C$, 60%RH with 'Rest', 'Exercise' and 'Recovery' period. The experimental garments consisted of briefs, undershirts(sleeveless), nightclothes, T-shirts, knee-trousers and socks. Subjects participated in two experiments, one was wearing near infrared lighted garments(NIR-O), the other was wearing regular garments(NIR-X). The order of experiment was randomized, and subjects wore experimental garments before 24 hours in order to benefit by near infrared light. Measurement items included rectal temperature ($T_{re}$), mean skin temperature ($\bar{T}_{sk}$), sweat rate, heart rate, oxygen uptake and subjective sensation. The results are as follows: As to the variation of rectal temperature and mean skin temperature, value of wearing NIR-X was higher than value of wearing NIR-O, indicating a significant level of difference (p<.001). Sweat rate under NIR-O and NIR-X condition were 575.35 g and 535.75 g, respectively. Heart rate value of NIR-X condition was higher than NIR-O. Oxygen uptake measured during experiment was the higher in NIR-X condition with significant difference (p<.001). In the subjective sensation, the value of NIR-O condition was higher than NIR-X condition without significantly difference.