Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Mass Production of Gain-of-Function Mutants of Hairy Roots in Catharanthus roseus

일일초에서의 기능획득 돌연변이 모상근의 대량생산

  • Ko, Suk-Min (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Chung, Hwa-Jee (Gendocs Inc.) ;
  • Lee, Hyo-Yeon (Subtropical Horticulture Research Institute, Jeju National University)
  • 고석민 (제주대학교 아열대 원예산업연구소) ;
  • 정화지 ((주)젠닥스) ;
  • 이효연 (제주대학교 아열대 원예산업연구소)
  • Received : 2011.07.14
  • Accepted : 2011.08.23
  • Published : 2011.10.31
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Abstract

This study describes conditions for the mass production of mutant hairy root lines by co-cultivation with A. rhizogenes harboring the activation tagging vector pHC7. Various sources of explants were subjected to genetic transformation with A. rhizogenes to determine optimum conditions and cultivar for the highest frequency of hairy root formation on explants. Hairy root formation also were investigated in transformed hairy roots grown in various culture media. Finally, a total of approximately 2,500 lines of hairy root mutants were produced in this study. A managing system for metabolomics in hairy root lines also were established. These hairy root lines will be useful to determine functions of genes relating biosynthesis pathway of secondary metabolites.

본 연구는 아그로박테리움을 이용한 기능획득 일일초 모상근의 대량생산을 위한 조건 확립에 대한 것이다. 본 연구에서는, 효율적인 형질전환 일일초 모상근 생산에 있어서의 최적의 일일초 품종의 선발과 최적의 일일초 조직을 결정하였으며, 또한 다양한 배지에 있어서의 모상근 유도를 조사하였다. 최종적으로 약 2,500개의 독립적인 형질전환 일일초 모상근 line을 생산하였으며, 또한 이들을 이용하여, 대사체 연구를 위한 효율적 관리 시스템을 구축하였다. 이들 모상근 line은 일일초 인돌알칼로이드 생합성 관련 유전자의 발굴 및 기능해석에 유용하게 쓰일 것이다.

Keywords

References

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