Korean Journal of Head & Neck Oncology (대한두경부종양학회지)

The Korean Society for Head and Neck Oncology (대한두경부종양학회)
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Analysis of (-)-Epigallocatechin-3-Gallate-Induced Apoptosis and Inhibition of Invasiveness in Oral Cavity Carcinoma Squamous Cell Carcinoma According to Expression of c-Met

구강암편평세포암에서 c-Met 발현여부에 따른 (-)-Epigallocatechin-3-Gallate의 세포사멸 및 종양침습억제효과의 변화분석

  • Shin, Yoo-Seob (Department of Otolaryngology, Ajou University School of Medicine) ;
  • Koh, Yoon-Woo (Department of Otolaryngology, Yonsei University College of Medicine) ;
  • Choi, Eun-Chang (Department of Otolaryngology, Yonsei University College of Medicine) ;
  • Kang, Sung-Un (Department of Otolaryngology, Ajou University School of Medicine) ;
  • Hwang, Hye-Sook (Department of Otolaryngology, Ajou University School of Medicine) ;
  • Choo, Oak-Sung (Department of Otolaryngology, Ajou University School of Medicine) ;
  • Lee, Han-Bin (Department of Otolaryngology, Ajou University School of Medicine) ;
  • Kim, Chul-Ho (Department of Otolaryngology, Ajou University School of Medicine)
  • 신유섭 (아주대학교 의과대학 이비인후과학교실) ;
  • 고윤우 (연세대학교 의과대학 이비인후과학교실) ;
  • 최은창 (연세대학교 의과대학 이비인후과학교실) ;
  • 강성운 (아주대학교 의과대학 이비인후과학교실) ;
  • 황혜숙 (아주대학교 의과대학 이비인후과학교실) ;
  • 추옥성 (아주대학교 의과대학 이비인후과학교실) ;
  • 이한빈 (아주대학교 의과대학 이비인후과학교실) ;
  • 김철호 (아주대학교 의과대학 이비인후과학교실)
  • Published : 2011.05.27
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Abstract

Hepatocyte growth factor(HGF) and c-Met play an important role in the control of tumor growth and invasion, and they are known to be good prognostic indicators of patient outcome. Epigallocatechin-3-gallate (EGCG) has been shown to have chemopreventive and therapeutic properties by modulating multiple signal pathways regarding the control of proliferation and invasion of cells. In this study, we evaluated the role of c-Met in EGCG-induced inhibition of invasion and apoptosis in an oral cancer cell line. In KB cells where c-Met was knocked down with siRNA, we performed invasion assay and FACS with Annexin V-FITC/PT staining. In addition, we checked the change of mitochondrial membrane potential(MMP) and the generation of reactive oxygen species(ROS). EGCG-induced inhibition of invasiveness was significantly decreased after the knock-down of c-Met. EGCG-induced apoptosis, MMP change and ROS generation was also reduced in c-Met knock-ed-down KB cells. These results suggest that c-Met is involved in EGCG-induced apoptosis and inhibition of invasiveness of oral cancer cell line.

Keywords

References

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