Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Inhibitory Effects of Nude Pack Containing Black Tea Water Extract on Skin Wrinkle Formation in Hairless Mice

홍차추출물 함유 누드팩의 Hairless 마우스 피부주름 형성 억제효과

  • Kim, Young-Chul (Department of Public Health, Graduate School, Keimyung University) ;
  • Park, Eun-Ye (Department of Public Health, Graduate School, Keimyung University) ;
  • Kim, Sang-Nam (Department of Public Health, Graduate School, Keimyung University) ;
  • Yoo, Yong-Gi (Department of Public Health, Graduate School, Keimyung University) ;
  • Park, Mi-Soon (Seoul Medical Science Institute) ;
  • Lee, Gui-Yeong (Department of Beauty Coordination, Keukdong College) ;
  • Lee, Suk-Jun (Department of Physical Education, Dankook University, Cheonan) ;
  • Chang, Byung-Soo (Department of Cosmetology, Hanseo University)
  • 김영철 (계명대학교 대학원 공중보건학과) ;
  • 박은예 (계명대학교 대학원 공중보건학과) ;
  • 김상남 (계명대학교 대학원 공중보건학과) ;
  • 유용기 (계명대학교 대학원 공중보건학과) ;
  • 박미순 (서울의과학연구소) ;
  • 이귀영 (극동정보대학 뷰티코디네이션과) ;
  • 이석준 (단국대학교 체육대학) ;
  • 장병수 (한서대학교 보건학부 피부미용학과)
  • Received : 2011.06.01
  • Accepted : 1011.06.23
  • Published : 2011.06.30
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Abstract

The aim of this study was to evaluate the inhibitory effect of nude pack containing black tea water extract (NPBT) on skin wrinkle formation in hairless mice. Skin wrinkles were induced by UVB irradiation to the backs of hairless mice for 5 weeks. And at the same time, NPBT was applied topically. Wrinkle formation, histological changes, expression of matrix metalloproteinase-3 (MMP-3) and protein activities of MMP-2 and MMP-9 were observed or analyzed. Wrinkles for the control group were formed as a pattern of deep furrows and thick crests. Whereas wrinkles for the NPBT treated group were formed as a pattern of shallow furrows and thin crests, and their wrinkle areas were significantly (p<0.001) lower than the control group. Collagen fibers were arranged irregularly and sparse in density and some elastic fibers were degenerated in the control group, while they were almost intact in the NPBT treated group. MMP-3 mRNA expression in the control group was significantly (p<0.001) higher than the normal group, and that of NPBT treated group was significantly (p<0.001) lower than the control group. The NPBT treated group showed remarkably lower protein activities of MMP-2 and MMP-9 than the control group. NPBT could have a considerable inhibitory effect on skin wrinkle formation in hairless mice.

홍차함유 누드팩의 피부주름 형성 억제효과를 알아보기 위해 SKH-1 hairless 마우스 등 부위에 UVB를 5주간 조사하여 주름을 유발시키고 이와 병행하여 홍차함유 누드팩을 도포한 실험에서 다음과 같은 결과를 얻었다. (1) 육안적인 피부주름 양상 비교에서, 대조군은 정상군에 비해 주름 능선의 두께가 굵고 간격이 넓으며 주름이 깊게 형성된 반면, retinoic acid를 도포한 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 상대적으로 주름 능선의 두께가 얇고 간격이 좁은 양상을 띠었으며 주름 총넓이 수치가 유의하게(p<0.001) 낮았다. (2) 피부의 조직학적 관찰에서, 대조군은 정상군에 비해 진피층내 교원섬유의 배열이 불규칙하고 양이 많이 줄어들었으며 변성된 탄력섬유가 군데군데 엉겨 있는 탄력섬유증이 관찰된 반면, 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 상대적으로 교원섬유의 배열이 규칙적이고 밀도가 조밀하며 변성된 탄력섬유의 양이 줄어 있었다. (3) 피부조직의 MMP-3 mRNA 발현량 측정 결과, 정상군에 비해 대조군은 유의하게(p<0.001) 높았으며 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 유의하게(p<0.001) 낮았다. MMP-2와 MMP-9 단백질 활성 측정 결과, 대조군은 정상군에 비해 현저하게 높은 반면, 양성대조군과 홍차함유 누드팩 도포군은 대조군보다 현저하게 낮게 나타났다. 본 연구를 통하여, 홍차함유 누드팩 도포군은 hairless 마우스에서 세포외 기질단백질의 분해 억제와 피부주름 형성 억제효과를 보였음을 확인하였다.

Keywords

References

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