Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지

Ultrastructural Variations on the Micropyle of Blacktip Grouper, Epinephelus Fasciatus before and after Artificial Fertilization

홍바리, Epinephelus fasciatus의 인공수정 전.후 난문의 미세구조적 변화

  • Kim, Seong-Hoon (Marine and Environmental Research Institute of Jeju National University) ;
  • Lee, Chi-Hoon (Marine and Environmental Research Institute of Jeju National University) ;
  • Ju, Hea-Sung (Marine and Environmental Research Institute of Jeju National University) ;
  • Kim, Hyung-Bae (Department of Marine Bio-resources, Gangwon Provincial University) ;
  • Lee, Young-Don (Marine and Environmental Research Institute of Jeju National University)
  • 김성훈 (제주대학교 해양과환경연구소) ;
  • 이치훈 (제주대학교 해양과환경연구소) ;
  • 주해성 (제주대학교 해양과환경연구소) ;
  • 김형배 (강원도립대학 해양생명과학과) ;
  • 이영돈 (제주대학교 해양과환경연구소)
  • Received : 2011.05.03
  • Accepted : 2011.06.23
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The micropyles on the prefertilized and artificial fertilized eggs of Epinephelus fasciatus were investigated using scanning electron microscope (SEM). The micropyles ($6.6{\pm}0.41\;{\mu}m$) of E. fasciatus eggs were found in the animal pole and theirs shape were observed as a flat crateriform of cylindrical shape. The micropylar vestibule arranged by 6~7 thickened spiral annuli on the ridge and contributed to differentiate and form fertilization cone for blocking to polyspermy by presenting swollen vestibule structure. As E. fasciatus eggs was pelagic, so chorionic surface was an uneven structures such as circular and fillar form nodules. Especially, various pores (0.15~0.55 ${\mu}m$, 230~270 pores) distributed at the only around micropyles, those pores radiately exhibited regular projection structures showing gill filament-shape. These ultrastructural characters of E. fasciatus eggs can be utilized in a taxonomical cue of grouper species.

홍바리, Epinephelus fasciatus 난의 인공수정 전 후 난문의 미세구조적 특징을 주사형 전자현미경으로 관찰하였다. 홍바리 난문($6.6{\pm}0.41\;{\mu}m$)은 원통형의 분화구형으로 동물극에 1개가 존재하였다. 난문의 vestibular ridge는 시계방향성을 갖는 6~7개의 나선형의 내벽구조로 구성되었다. 난문의 내벽구조물인 나선형의 vestibular ridge는 인공수정 후 부풀어 올라와 돔(dome)형의 수정구로 분화 형성되었다. 홍바리 난은 부상란으로 난막표면에는 pore 가장자리에 고리형의 nodule과 pore 사이에 돌기형의 nodule이 관찰되었으나, 부착사와 유사한 보조 구조물은 관찰할 수 없었다. 난문 주변부에는 동심원상으로 크기가 다른 pore (0.15~0.55 ${\mu}m$)들이 개구된 형태로 혼재하였고, 수정 후 돔(dome)형의 수정구가 분화 형성되면서 pore들이 닫히는 현상을 보였다. 홍바리 난문의 미세구조적 특징은 바리과 어종간에 형태학적으로 상이하여, 바리과 어류의 계통분류학의 형질로 이용될 수 있다고 판단된다.

Keywords

References

  1. Berois N, Arezo MJ, Chalar C, Brauer MM, Barros C: Ultrastructure and protein composition of the oocyte envelope in the white mouth croaker (Micropogonias furnieri, Desmarest, 1823, Sciaenidae, Perciformes). J Appl Ichthyol 23 : 34-39, 2007. https://doi.org/10.1111/j.1439-0426.2006.00762.x
  2. Bless R, Riehl R: Biology and egg morphology of the Damatian barbelgudgeon, Aulopyge huegeli, an endangered endemic species in Croatia. Environ Biol Fish 63 : 451-456, 2002. https://doi.org/10.1023/A:1014992626062
  3. Breining T, Britz R: Egg surface structure of clingfish species, using scanning electron microscopy. J Fish Biol 56 : 1129-1137, 2000. https://doi.org/10.1111/j.1095-8649.2000.tb02128.x
  4. Chen KC, Shao KT, Yang JS: Using micropylar ultrastructure for species identification and phylogenetic inference among four species of Sparidae. J Fish Biol 55 : 288-300, 1999. https://doi.org/10.1111/j.1095-8649.1999.tb00679.x
  5. Cherr GN, Clar WHJr: An egg envelope component induces the acrosome reaction in sturgeon sperm. J Exp Zool 234 : 75-85, 1985. https://doi.org/10.1002/jez.1402340110
  6. Chiou LM, Chung MC, Tung PG, Hsu T, Yang JS: The use of egg chorion glycoprotein of Epinephelus malabaricus for egg identification. J Fish Biol 65 : 1614-1621, 2004. https://doi.org/10.1111/j.0022-1112.2004.00571.x
  7. Dettlaff TA, Ginsburg AS, Schmalhausen O: Sturgeon Fishes: Developmental Biology and Aquacutlrue. Springer-Verlag, Berlin, p. 300, 1993.Deung YK, Kim DH, Reu DS: Ultrastructure of the fertilized egg envelop in Pale chub Cyprinidae, teleost. Kor J Electron Microscopy 30 : 321-326, 2000. (Korean)
  8. Esmaeili HR, Johal MS: Ultrastructural features of the egg envelope of silver carp, Hypophthalmichtys molitrix (Osteinchthys, Cyprinidae). Environ Biol Fish 72 : 373-377. 2005. https://doi.org/10.1007/s10641-004-2589-y
  9. Giulianini PG, Ferrero EA: Ultrastructural aspects of the ovarian follicle and egg envelope of the sea-grass goby Zosterisessor ophiocephalus (Osteichthyes, Gobiidae). Ital J Zool, 68 : 29-37, 2000.
  10. Guraya SS: The cell and molecular biology of fish oogenesis. Karger, New York, pp. 141-147, 1986.
  11. Hart NH: Fertilization in teleost fishes: mechanisms of sperm-egg interactions. Int Rev Cytol 121 : 1-66, 1990.
  12. Hirai A, Yamamoto ST: Micropyle in the developing of the anchovy, Engraulis japonica. Japan J Ichthyol 33 : 62-66, 1986.
  13. Kim DH, Deung YK, Kim HY, Reu DS: Ultrastructure of the fertilized egg envelope from long nose barbel, Cyprinidae, teleost. Kor J Electron Microscopy 31 : 85-90, 2001. (Korean)
  14. Kim DH, Reu DS, Deung YK: Ultrastructure of the fertilized egg envelope from Dark sleeper, Eleotrididae, Teleost. Kor J Electron Microscopy 32 : 39-44, 2002. (Korean)
  15. Kobayashi W, Yamamoto TS: Fine structure of micropylar apparatus of the chum salmon egg with a discussion of the mechanism for blocking polyspermy. J Exp Zool 217 : 265-275, 1981. https://doi.org/10.1002/jez.1402170213
  16. Laale HW: The perivitelline space and egg envelopes of bony fishes: a review. Copeia 2 : 210-226, 1980.
  17. Li YH, Wu CC, Yang JS: Comparative ultrastructural studies of the zona radiata of marine fish eggs in three genera in Peciformes. J Fish Biol 56 : 615-621, 2000. https://doi.org/10.1111/j.1095-8649.2000.tb00759.x
  18. Linhart O, Kudo S: Surface structure of paddlefish eggs before and after fertilization. J Fish Biol 51 : 573-582, 1997.
  19. Mekkawy IAA, Osman AGH: Ultrastructural studies of the morphological variations of the egg surface and envelopes of the African catfish, Clarias gariepinus (Burchell, 1982) before and after fertilisation, with a discussion of the fertilization mechanism. Sci Mar 702 : 23-40, 2006.
  20. Mikodina EV: Surface structure of the egg of teleostean fishes. Voprosy Ikhtiologii 1 : 106-113, 1987.
  21. Riehl R: Micropyle of some salmonids and coregonids. Environ Biol Fish 5 : 59-66, 1980. https://doi.org/10.1007/BF00000950
  22. Riehl R, Kock KH: The surface structure of Antarctic fish eggs and its use in identifying fish eggs from the southern ocean. Polar Biol 43 : 617-620, 1989.
  23. Riehl R, Kokoscha M: A unique surface pattern and micropylar apparatus in the eggs of Luciocephalus sp. (Perciformes, Luciocephalidae). J Fish Biol 43 : 617-620, 1993. https://doi.org/10.1111/j.1095-8649.1993.tb00444.x
  24. Rizzo E, Sato Y, Barreto BP, Godinho HP: Adhesiveness and surface patterns of eggs in neotropical freshwater teleosts. J Fish Biol 61 : 615-632, 2002. https://doi.org/10.1111/j.1095-8649.2002.tb00900.x
  25. Stanley HP: Fine structure of spermatogenesis in the elasmobranch fish Squalussuckleyi. J Ultrastruct Res 36 : 86-102, 1971. https://doi.org/10.1016/S0022-5320(71)80090-4
  26. Vorobyeva EI, Markov KP: Specific ultrastructural features of eggs of Acipenseridae in relation to reproductive biology and phylogency. J Ichthyol 39 : 157-169, 1999.