The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지
DOI QR코드

DOI QR Code

The Antioxidative Effects and Isolation and Characterization of the Extracts from Morus alba L.

뽕잎 추출물의 항산화 효과와 항산화 성분 분리 및 동정에 관한 연구

  • Jeon, Ye-Sook (Dept. of Food and Nutrition, Chungwoon University) ;
  • Kim, Mi-Won (Dept. of Food and Nutrition, Chungwoon University)
  • 전예숙 (청운대학교 식품영양학과) ;
  • 김미원 (청운대학교 식품영양학과)
  • Received : 2011.02.09
  • Accepted : 2011.03.28
  • Published : 2011.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study identified the formula of the antioxidant substance separated from the ethyl acetate and the butanol extract and tested the antioxidant properties with the electron donating ability(EDA). Each phase with the fractionated methanol extract from mulberry leaf was screened in advance for the antioxidant substance with EDA. As the result, activity appeared in the ethyl acetate and butanol phase and the antioxidant component was separated. As the consequence, 2 components from the ethyl acetate phase and 1 from the butanol phase were separated, among which the structures of the components from ethyl acetate were determined by wogonin and linarin, whereas the structure of the component from the butanol phase was determined by pectolinarin. In the screening of antioxidant activity by the scavenging effect of the DPPH radical, the wogonin and linarin components from ethyl acetate phase showed more powerful antioxidant property than the component from butanol. The results from this study indicate that the chemical compound separated from the ethyl acetate extract has more powerful antioxidant property than the one separated from the butanol extract. The components separated from the ethyl acetate extract were wogonin and linarin, which are flavonoids, whereas the component from butanol was pectolinarin. Therefore, this study suggested that the feasibility of mulberry leaf as a functional food additive and its value as a natural antioxidant is very high.

Keywords

References

  1. 우원식. 1997. 천연물화학 연구법. pp.1-60. 서울대학교출판부
  2. Cha NY, Kim HJ, Chung CH, Cho YS. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J Korean Soc Food Sci Nutr 28:1310-1315
  3. Chang HJ, Noh KL. 2000. Screening of electron donating ability antibacterial and nitrits scavenging effect of some herbal extracts. Korean J Food Cookery Sci 16:372-377
  4. Cho YJ, Ju IS, Kim BO, Kim JH, Lee BG. 2007. The antimicrobial activity against Helicobacter pylori and antioxidant effect from the extracts of mulberry leaves. J Korean Soc Appl Biol Chem 50:334-343
  5. Dietz BM, Kang YH, Liu G, Eggler AL, Yao P, Chadwick LR, Pauli GF, Farnsworth NR, Mesecar AD, Bolton JL. 2005. Xanthohumol isolated from Humilus lupulus inhibits menadioneinduced DNA damage through induction of quinone reductase. Chemical Research in Toxicology 18:1296-1305 https://doi.org/10.1021/tx050058x
  6. Do JC, Chai JY, Son JK. 1991. Studied on the consitituents of Lycopus lucidus(II). Kor J Pharmacogn 22:166-170
  7. Goudard M, Bonvin JF, Lebreton P, Chopin J. 1978. Differentiation des dihydroxy-5,7 methoxy-6 ou 8 flavones, flavonols et mehyl-3 flavonols par spectrometrie de masse. Phytochemistry 17:145-146 https://doi.org/10.1016/S0031-9422(00)89698-3
  8. Inagaki I, Hisada S, Shima K. 1971. Studies on the constituents of anodendron affine durce. I. Isolation of wogonin, dambonitol, sucrose and some other components from stems. Yakugaku Zasshi 91:1133-1136
  9. Ishida H, Umino T, Tsuji K, Kosuge T. 1987. Studies on antihemorrhagic substances in herbs classified as hemostatics in chinese medicine. VII. On the antihemorrhagic principle in Cirsium japonicum DC. Chem Pharm Bull 35:861-864 https://doi.org/10.1248/cpb.35.861
  10. Jeong CH, Joo OS, Shim KH. 2002. Chemical components and physiological activities of young mulberry stem. J. Korea Food Preserv 9:228-233
  11. Ju MJ, Kwon JH, Kim HK. 2009. Physiological activities of mulberry leaf and fruit extracts with different extraction conditions. Korean J Food Preserv 16:442-448
  12. K. R. Markham. 1982. Techniques of Flavonoid Identification. Academic Press, pp.1-45. London
  13. Kang PD, KimJW, Sohn BH, Kim KY, JungIY, Kim MJ, Ryu KS. 2006. Accumulating pattern of a-glucosidase inhibitor various silkworom varities. J Seric. Sci 48:25-27
  14. Karen J, Pamela J. 1991. Extraction and identification of antioxidants in oats. JAOCS 86:365-370
  15. Kim HB, Kang CK, Sung GB, Kang SW, Lee JR. 2007. Antioxid ative capacity of mulberry leaf and its tea. Korean J Seric Sci 49:18-23
  16. Kim HB, Koh SH, Seok YS. 2006. Anti-oxidative effect of 'Cheongilppong' with mulberry leaves according to different collection areas and some kinds of mulberry branches. Korean J Seric Sci 48:41-45
  17. Kim HB, Lee YW, Lee WJ, Moon JY. 2001. Physiological effects and sensory characteristics of mulberry fruit wine with chongilppong. Korean J Seris Sci 43:16-20
  18. Kim HB. 2005. Antioxidant capacity analysis of water-soluble substances according to maturity stages in yield-type mulberry leaves and fruits collected from Kang-Won province. Korean J Seric Sci 47:93-98
  19. Kim SS, Jeong MH, Seo YC, Kim JS, Kim NS, Woon WB, Ahn JH, Hwang B, Park DS, Park SJ, Lee HY. 2010. Comparison of antioxidant activities by high pressure extraction of Codonopsis lanceolata from different production areas. Korean J Medicinal Crop Sci 18:248-254
  20. Lee HB, Kwak JH, Zee OP, Yoo SJ. 1994. Flavonoids from Circum rhinoceros. Arch Pharm Res 17:273-277 https://doi.org/10.1007/BF02980460
  21. Lee HS, Chung KS, Kim SY, Ryu KS, Lee WC. 1998. Effect of several sericultural products on blood glucose lowering for Alloxan-induced hyperglycemic mice. Korean J Seric Sci 40:38-42
  22. Lee JM, Kim JA, Lee MJ. 2002. Sensory and physicochemical attributes of boogags using mulberry leaf. Korean J Dietary Culture 17:103-110
  23. Marlham. 1982. Techniques of Flavonoid Identification. Academic Press. London
  24. Park BH, Back KY, Lee SI, Kim SD. 2008. Quality and antioxidative characteristics of Cudrania tricuspidata leaves tea. Korean J Food Preserv 15:461-468
  25. Takido M, Aimi M, Takahashi S, Yamanouchi S, Torii H, Dohi S. 1975. Studies on the constituents in the water extracts of crude drugs. I. On the roots of Scutellaria baicalensis georgi(Wogon)1. Yakugaku Zasshi 95:108-113
  26. Toshiko S, Ikuyo T, Noriko H, Taketoshi K. 1994. Thermal stability of chiorophyll in green leaves. 調理科學 27:126-131

Cited by

  1. Effects of Dietary Supplementation of Mulberry Leaves and Silkworm Excreta Extracts on Weight Performance, Blood Characteristics and Cecal Microflorae of Chicks vol.50, pp.2, 2012, https://doi.org/10.7852/jses.2012.50.2.150
  2. Anti-diabetic effect of the mixture of mulberry leaf and green tea powder in rats with streptozotocin-induced diabetes vol.21, pp.4, 2014, https://doi.org/10.11002/kjfp.2014.21.4.549
  3. 상엽(桑葉) 추출물의 Adipogenesis 억제를 통한 항비만 활성 평가 vol.31, pp.5, 2011, https://doi.org/10.6116/kjh.2016.31.5.47.
  4. 3T3-L1 세포에서 상엽이 유발하는 AMPK signaling pathway를 통한 adipogenesis 억제에 관한 연구 vol.27, pp.2, 2011, https://doi.org/10.5352/jls.2017.27.2.155
  5. 뽕잎 분말을 첨가한 쿠키의 품질특성 vol.32, pp.6, 2011, https://doi.org/10.7318/kjfc/2017.32.6.558
  6. C2C12 근관세포에서 상엽에 의한 AMPK의 불활성화와 AICAR로 유도된 근위축 억제의 연관성에 관한 연구 vol.28, pp.4, 2011, https://doi.org/10.5352/jls.2018.28.4.435
  7. 연잎, 상엽, 건조 무 혼합 추출물의 지표성분 wogonin, quercetin 및 quercetin-3-O-glucuronide의 분석법 개발 및 검증 vol.33, pp.4, 2011, https://doi.org/10.13103/jfhs.2018.33.4.289
  8. 뽕나무(Morus alba)와 꾸지뽕나무(Cudrania tricuspidata)의 부위에 따른 항산화 활성 및 3T3-L1세포 지방축적 억제 효과 vol.32, pp.2, 2011, https://doi.org/10.9799/ksfan.2019.32.2.138
  9. Isolation and Biological Properties of the Natural Flavonoids Pectolinarin and Pectolinarigenin—A Review vol.9, pp.7, 2011, https://doi.org/10.3390/antibiotics9070417