YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Antitumor Effect of $18{\beta}$-Glycyrrhetinic Acid against Human Tumor Xenografts Caused by A549 Cancer Cell

A549 암세포 기인성 종양에 대한 $18{\beta}$-Glycyrrhetinic Acid의 항종양효과

  • Kim, Ha-Yan (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University) ;
  • Kim, Song-Yi (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University) ;
  • Lee, Jue-Hee (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University) ;
  • Han, Yong-Moon (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University)
  • 김하얀 (동덕여자대학교 약학대학 면역미생물학교실) ;
  • 김송이 (동덕여자대학교 약학대학 면역미생물학교실) ;
  • 이주희 (동덕여자대학교 약학대학 면역미생물학교실) ;
  • 한용문 (동덕여자대학교 약학대학 면역미생물학교실)
  • Received : 2010.09.28
  • Accepted : 2010.12.13
  • Published : 2011.02.28
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Abstract

Many reports indicate that $18{\beta}$-glycyrrhetinic acid ($18{\beta}$-GA) from Glycyrrhizae Radix has anti-inflammatory and immunoregulatory activities, whereas reports regarding anticancer activity of the compound are few. In present study, we investigated antitumor effect of $18{\beta}$-GA on tumor caused by A549 cancer cell in mice. Data resulting from the cytotoxicity assay showed that $18{\beta}$-GA caused killing of A549 cells. $LD_{50}$ values of $18{\beta}$-GA were app. 180 ${\mu}M$ and 80 ${\mu}M$, corresponding to 48 hr- and 72 hr-treatments, displaying that the killing activity was more effective as the $18{\beta}$-GA treatment was prolonged. Based on these data, antitumor effect of $18{\beta}$-GA was tested in nude mice. For induction of the tumor, A549 ($3{\times}10^6$ cells/mouse) was injected subcutaneously into the lateral abdomen of nude mice (Balb/c nu/nu). To determine the antitumor effect, nude mice with tumor were given $18{\beta}$-GA (1 mg/200 ${\mu}l$/mouse) intraperitoneally every three days for four times. Tumor-sizes were measured with a caliper for a period of 24 days. Results showed that the $18{\beta}$-GA treatment reduced the tumor-sizes (P<0.05) as compared with negative control nude mice that received diluent (DPBS). The reduction degree was greater than reduction degree by doxorubicin (60 ${\mu}g$/mouse), and the pattern of reduction was almost sustained during the entire period of the observation. In conclusion, our studies demonstrate that $18{\beta}$-GA has antitumor activity to the A549 cancer cell-caused tumor.

Keywords

References

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