Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Expression of Catalase (CAT) and Ascorbate Peroxidase (APX) in MuSI Transgenic Tobacco under Cadmium Stress

  • Kim, Kye-Hoon (Department of Environmental Horticulture, The University of Seoul) ;
  • Kim, Young-Nam (Department of Environmental Horticulture, The University of Seoul) ;
  • Lim, Ga-Hee (Department of Environmental Horticulture, The University of Seoul) ;
  • Lee, Mi-Na (Department of Environmental Horticulture, The University of Seoul) ;
  • Jung, Yoon-Hwa (Department of Environmental Horticulture, The University of Seoul)
  • Received : 2011.01.20
  • Accepted : 2011.02.25
  • Published : 2011.02.28
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Abstract

The MuSI is known as a multiple stress resistant gene with several lines. A previous study using RT-PCR showed that the expression of MuSI gene in tobacco plant induced its tolerance to Cd stress. This study was conducted to examine the enhanced Cd tolerance of the MuSI transgenic tobacco plant through germination test and to understand the role of the involved antioxidant enzymes for the exhibited tolerance. Germination rate of MuSI transgenic tobacco was more than 10% higher than that of wild-type tobacco, and seedlings of MuSI transgenic tobacco grew up to 1.6 times larger and greener than seedlings of wild-type tobacco at 200 and 300 ${\mu}M$ Cd. From the third to the fifth day, CAT activities at 100 and 200 ${\mu}M$ Cd and APX activities at 100, 200 and 300 ${\mu}M$ Cd of MuSI transgenic tobacco were up to two times higher than those of wild-type tobacco. MuSI gene is shown to enhance the activities of antioxidant enzymes resulting in higher tolerance to oxidative stress compared with the control plant.

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References

  1. Adriano, D.C. 1986. Trace element in the terrestrial environment. Springer-Verlag, New York.
  2. Aebi, H. 1984. Catalase in virto: H. U. Bergmeyer and K. Gawehn (Ed) Methods of Enzymatic Analysis. Verleg Chemie, Weinheim. p. 673-684.
  3. Bradford, M.M. 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry 72:248-254.
  4. Cherian, S. and M. Margarida Oliveira. 2005. Transgenic Plants in Phytoremediation: Recent Adavance and New Possibilities. Environmental Science & Technology 39 (24): 9377-9390. https://doi.org/10.1021/es051134l
  5. Dixit, V., V., Pandey, and R. Shyam. 2001. Differential antioxidative responses to cadmium in roots and leaves of pea (Pisumsativum L. cv. Azad). Journal of Experimental Botany 358:1101-1109.
  6. Kabata-Pendias, A. and A.B. Mukherjee. 2007. Trace Elements from Soil to Human. Springer p. 294-308.
  7. Kim, Y.N. 2010. Potential use of MuSI transgenic tobacco for phytoremediation of the soils contaminated with cadmium. MS. Thesis. The University of Seoul.
  8. Markovska, Y.K., N.I. Gorinova, M.P. Nedkovska, and K.M. Miteva. 2009. Cadmium-induced oxidative and antioxidant responses in Brassica juncea plants. Biologia Plantarum 53:151-154. https://doi.org/10.1007/s10535-009-0023-1
  9. Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7:405-410. https://doi.org/10.1016/S1360-1385(02)02312-9
  10. Nakano, Y. and Y.K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology 22(5):867-880.
  11. Sawada, H., I.S. Shim, K. Usui, K. Kobayashi, and S. Fujihara. 2008. Adaptative mechanism of Echinochloa crus-galli Beauv. var formosensis Ohwi under salt stress: Effect of salicylic acid on salt sensitivity. Plant Science 174:583-589. https://doi.org/10.1016/j.plantsci.2008.03.013
  12. Schützendüble, A., P. Schwanz, T. Teichmann, K. Gross, R. Langenfeld-Heyser, D.L. Godbold, and A. Polle. 2001. Cadmium-induced Changes in Antioxidative Systems, Hydrogen Peroxide Content, and Differentiation in Scots Pine Roots. Plant Physiology 127:887-898. https://doi.org/10.1104/pp.010318
  13. Schützendüble, A. and A. Polle. 2002. Plant reponses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. Journal of Experimental Botany 53:1351-1365. https://doi.org/10.1093/jexbot/53.372.1351
  14. Seo, S.G., J.S. Kim, Y.S. Yang, B.K. Jun, S.W. Kang, G.P. Lee, W. Kim, J.B. Kim, H.U. Lee, and S.H. Kim. 2010. Cloning and characterization of the new multiple stress responsible gene I (MuSI ) from sweetpotato. Genes & Genomics 32:544-552. https://doi.org/10.1007/s13258-010-0093-7
  15. Vitoria, A.P., P.J. Lea, and R.A. Azevedo. 2000. Antioxidant enzymes reponses to cadmium in radish tissues. Phytochemistry 57:701-710.
  16. Yamazaki, K. 1982. Nutrient Solution Culture (in Japanese). p. 251. Pak-kyo Co., Tokyo, Japan.

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