Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Investigation of Siderophore production and Antifungal activity against Phytophthora capsici as related to Iron (III) nutrition by Lysobacter antibioticus HS124

  • Ko, Hyun-Sun (Department of Biological Chemistry, Chonnam National University, Environment-Friendly Agriculture Research Center) ;
  • Tindwa, Hamisi (Department of Biological Chemistry, Chonnam National University, Environment-Friendly Agriculture Research Center) ;
  • Jin, Rong De (Agricultural Environment and Resources Research Center, Jilin Academy of agricultural Sciences) ;
  • Lee, Yong-Seong (Department of Biological Chemistry, Chonnam National University, Environment-Friendly Agriculture Research Center) ;
  • Hong, Seong-Hyun (Department of Biological Chemistry, Chonnam National University, Environment-Friendly Agriculture Research Center) ;
  • Hyun, Hae-Nam (Major of plant resources & environment, Cheju National University) ;
  • Nam, Yi (Ansung Training Institute, National Agricultural Cooperative Federation) ;
  • Kim, Kil-Yong (Department of Biological Chemistry, Chonnam National University, Environment-Friendly Agriculture Research Center)
  • Received : 2011.07.20
  • Accepted : 2011.08.16
  • Published : 2011.08.31
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Abstract

Lysobacter antibioticus HS124 isolated from pepper rhizosphere soil produced catechol type siderophore. Purified siderophore by Diaion HP-20 and silica gel column chromatography showed several hydroxyl functional groups adjacent to benzene rings by analysis of $^1H$ NMR spectroscopy. The strain HS124 showed different activities to suppress Phytophthora capsici with different concentrations of exogenous Fe (III) in minimal medium where antifungal activity with $100{\mu}M$ Fe (III) was approximately 1.5 times higher than in absence of Fe (III). Bacterial population in this Fe (III)-amended medium was also highest with $8.9{\times}10^8\;CFU\;ml^{-1}$ which also corresponded to the strongest siderophore activity. When grown in rich medium (minimal medium with N, $P_2O_5K_2O$ and glucose), HS124 exhibited approximately 2 times stronger antifungal activity compared to minimal medium. In pot trials, treatments of bacterial culture grown in rich medium with (C1) or without (C2) $100{\mu}M$ Fe (III) exhibited a high protection of pepper plants from disease, compared to medium only with (M1) or without (M2) $100{\mu}M$ Fe (III). Especially, treatment C1 showed the best disease control effect of about 70 %. Thus, the strain HS124 should be recommended as a potential biocontrol agent against P. capsici in pepper.

Keywords

References

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