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Protective Effect of Processed Saengmaek-san(SM) on Cell Damage in UV-exposed HaCaT Cell

생맥산(生脈散)이 자외선에 의한 피부각질세포의 상해에 미치는 영향

  • Kim, Eun-Seop (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University) ;
  • Yoo, Dong-Youl (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
  • 김은섭 (대전대학교 한의과대학 부인과 교실) ;
  • 유동열 (대전대학교 한의과대학 부인과 교실)
  • Received : 2011.04.30
  • Accepted : 2011.05.11
  • Published : 2011.05.28

Abstract

Objectives: This study was performed to assess the protective effect of Saengmaek-san (SM) on UVB-induced HaCaT cell damage. Methods: The protective effects of Saengmaek-san(SM) were determined by UVB-induced HaCaT assay. We assessed protective effects of Saengmaek-san (SM) on LDH release and nitrite release from HaCaT. And COX-2, Bcl-2, Bax, $TNF{\alpha}$, c-jun, c-fos, NF-kB, iNOS, Bcl-xL gene expression were determined in HaCaT using real-time PCR method. Results: 1. SM inhibited LDH-release, nitrite production in UVB-exposed HaCaT. 2. SM suppressed the gene expression of COX-2, $TNF{\alpha}$ in UVB-exposed HaCaT. 3. SM increased the gene expression of Bcl-2, Bax, Bcl-xL family protein in UVB-exposed HaCaT. 4. SM suppressed the gene expression of c-jun, c-fos, NF-kB in UVB-exposed HaCaT. Conclusions: The study showed SM inhibited the cell damage in UVB-exposed HaCaT.

Keywords

References

  1. 대한피부과학회 교과서 편찬위원회. 피부과학 제5판. 서울:여문각. 2008:12-8, 131-6.
  2. 노석선. 피부과학. 서울:아이비씨기획. 2006:11, 31, 90, 607.
  3. Fisher GJ et al. Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J med. 1997;337:1419-28. https://doi.org/10.1056/NEJM199711133372003
  4. Yamamoto Y. Role of active oxygen species and antioxidants in photoaging. J Dermatol Sci. 2001;27:S1-S4.
  5. Sung Mok Beak, Yong Soo Lee, Jung-Ae Kim. NADPH oxidase and cyclooxygenase mediate the ultraviolet B-induced generation of reactive oxygen species and activation of nuclear factor-kB in HaCaT human keratinocytes. J Biochem. 2004;86:425-9. https://doi.org/10.1016/j.biochi.2004.06.010
  6. Masaki H. Atsumi T. Sakurai H. Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation. Biochem Biophys Res Commun. 1995; 206:474-9. https://doi.org/10.1006/bbrc.1995.1067
  7. Karin M. Liu ZG, Zandi E. AP-1 function and regulation. Curr Opin Cell Biol. 1997;9:240-6. https://doi.org/10.1016/S0955-0674(97)80068-3
  8. Barber LA et al. Expression of the platelet-activating receptor results in enhanced ultraviolet B radiation-induced apoptosis in a human epidermal cell line. J Biol chem 1998;273:18891-7. https://doi.org/10.1074/jbc.273.30.18891
  9. Lee KS et al. Melatonin reduces ultraviolet-B induced cell damages and polyamine levels in human skin fibroblasts in culture. Exp Mol Med. 2003;35(4):263-8. https://doi.org/10.1038/emm.2003.35
  10. Cha HJ et al. Anti-invasive activity of ursolic acid correlates with the reduced expression of matrix metalloproteinase-9 (MMP-9) in HT1080 human fibrosarcoma cells. Cancer Res. 1996;56:2281-4.
  11. Brenneisen P et al. Central role of ferrous/ferric iron in the ultraviolet B irradiation-mediated signaling pathway leading to increased interstitial collagenase (matrix-degrading metalloproteinase (MMP)-1) and stromelysin-1(MMP-3) mRNA levels in cultured human dermal fibroblasts. J Biol Chem. 1998; 273, 5279-87. https://doi.org/10.1074/jbc.273.9.5279
  12. 이동원. 동원십종의서. 서울:대성문화사. 1983:90-1.
  13. 허준. 동의보감. 서울:남산당. 1999:410.
  14. 신재용. 방약합편해설. 서울:성보사. 1991:27-8.
  15. 한의과대학 방제학교실. 방제학. 서울 :영림사. 1999:283-4.
  16. 김세길. 생맥산이 백서의 심혈관계에 미치는 영향. 원광대학교 대학원. 1986.
  17. 김석. 생맥산투여가 흰쥐 근육 glycogen함량 및 산소활성에 미치는 영향에 대한 조직학적 연구. 대한한의학회지. 1990;11(1):156-64.
  18. 신대철. 생맥산이 심혈관계 및 국소뇌혈류량에 미치는 영향. 대한내과학회지. 1999;20(1):153-66.
  19. 손정희. 생맥산가감방이 생쥐의 면역반응에 미치는 영향. 대한한방부인과학회지. 2003;16(3):162-73.
  20. Ickkawa H. Rold of component herbs in antioxidant activity of shengmai san - a traditional chinese medicine formula preventing cerebral oxidative damage in rat. Am J Chin Med, 2003;31(4):509-21. https://doi.org/10.1142/S0192415X03001193
  21. Wand L. Prevention of oxidative injury in PC12 cells by a traditional chinese medicine Shengmai San, as a model of an antioxidant-based composite formula. Biol Pharm Bull. 2003;26(3) :1000-4. https://doi.org/10.1248/bpb.26.1000
  22. 김현경. 생맥산의 방사선 보호효과 -생쥐 소낭세포 재생과 Apoptosis에 미치는 영향. 대한한방내과학회지. 2006; 27(2):316-26.
  23. 王氷注. 黃帝內經素問. 서울:大成出版社. 1986:21, 101, 212, 274, 340, 768.
  24. 이향우, 김주연, 이연희. 피부과학. 서울:광문각. 2003:23.
  25. 이지해 외. Hairless mice에서의 대두홍삼 혼합 분말(APB-03)의 경구 반복투여 시 피부 주름 생성 예방 효과. 한국식품과학회지. 2005;37(6):989-96.
  26. Sander CS et al. Phothoaging is associated with protein oxidation in human skin in vivo. J Invest Dermatol. 2002;618-5.
  27. Ozeki Y, Davies E, Takeda J. Structure and expression of chalcone synthesis gene in carrot suspension cultured cells regulated by 2.4-D. Plant Cell Physiology. 1993;34(7):1029-37.
  28. 김형우 등. 포공영 추출물의 항산화 효과 및 피부 각질세포 보호효과. 대한본초학회지. 2009;24(3):103-8.
  29. 이진영 등. 황기의 자외선에 의한 세포손상을 막는 보호효과. 생약학회지. 2008;39(4):300-4.
  30. Sun W et al. Catalase activity of chloroperoxidase and its interaction with peroxidase activity. Biochem Cell Biol. 1994;72(7-8):321-31. https://doi.org/10.1139/o94-045
  31. Lardinois OM. Rouxhet PG. Peroxidatic degradation of azide by catalase and irreversible enzyme inactivation. Biochim Biophys Acta. 1996;1298(2):180-90. https://doi.org/10.1016/S0167-4838(96)00130-6
  32. Lardinois OM. Reactions of bovine liver catalase with superoxide radicals and hydrogen peroxide. Free Radic Res. 1995;22(3):251-74. https://doi.org/10.3109/10715769509147544
  33. 조재외. HaCaT 각질형성세포에서 자외선 B에 의하여 유도되는 염증성사이토카인에 대한 Curcumin의 항염증 효과. 대한피부과학회지. 2009;47(2) :121-6.
  34. 중도일. 피부과의 한방치료. 서울:일중사. 1991:40.
  35. 본초학교수 공편저. 본초학 서울:영림사. 2003:532, 589, 623.
  36. Song Xiu-zu, BI Zhi-gang, XU Ai-e. Green tea polyphenol epigallocatechin -3-gallate inhibits the expression of nitric oxide synthase and generation of nitric oxide induced by ultraviolet B in HaCaT cells. Chin Med J. 2006; 119:282-7.
  37. Weller R. Nitric oxide - a key mediator in cutaneous physiology. Clin Exp Dermatol. 2003;28:511-4. https://doi.org/10.1046/j.1365-2230.2003.01365.x
  38. Jae-We Cho et al. Curcumin inhibits the expression of COX-2 in UVBirradiated human keratinocytes(HaCaT) by inhibiting activation of AP-1: p38 MAP kinase and JNK as potential upstream targets. Experimental and Molecular Med. 2005;37(3):186-92. https://doi.org/10.1038/emm.2005.25
  39. An Van Laethem, Marjan garmyn, Patrizia Agostinis. Starting and propagating apoptotic signals in UVB irradiated keratinocytes. Photochemical & Photobiological Sci. 2009;8:299-308. https://doi.org/10.1039/b813346h
  40. Marcheko ND et al. Death signalinduced localization of p53 protein to mitochondria. A potential role in apoptotic signaling. J Bio Chem. 2000;275(16):202-12.
  41. JE Chipuk et al. Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis. Science. 2004;303:1010-4. https://doi.org/10.1126/science.1092734
  42. V Chaturvedi et al. Resistance to UVinduced apoptosis in human keratinocytes during accelerated senescence is associated with functional inactivation of p53. J. Cell Physiol. 2004;198:100-9. https://doi.org/10.1002/jcp.10392
  43. Yong Cui et al. Involvement of ErK and p38 MAP kinase in AAPHinduced COX-2 expression in HaCaT cells. Chem and Phys of Lipids. 2004;129:43-52. https://doi.org/10.1016/j.chemphyslip.2003.11.004
  44. Choi AMK. et al. Alternations in the molecular response to DNA damage during cellular aging of cultured fibroblasts: reduced AP-1 activation and collagenase gene expression. J Cell Physiol. 1995;164:65-73. https://doi.org/10.1002/jcp.1041640109
  45. Angel P, Karin M. The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation. Biochim Biophys Acta. 1991;1072:129-57.
  46. Fisher GJ et al. c-Jun-dependent inhibition of cutaneous procollagen transcription following ultraviolet irradiation is reversed by all-trans retinoic acid. J Cret Itvest. 2000; 106:663-70.