The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Protective Effect of Processed Saengmaek-san(SM) on Cell Damage in UV-exposed HaCaT Cell

생맥산(生脈散)이 자외선에 의한 피부각질세포의 상해에 미치는 영향

  • Kim, Eun-Seop (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University) ;
  • Yoo, Dong-Youl (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
  • 김은섭 (대전대학교 한의과대학 부인과 교실) ;
  • 유동열 (대전대학교 한의과대학 부인과 교실)
  • Received : 2011.04.30
  • Accepted : 2011.05.11
  • Published : 2011.05.28
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Abstract

Objectives: This study was performed to assess the protective effect of Saengmaek-san (SM) on UVB-induced HaCaT cell damage. Methods: The protective effects of Saengmaek-san(SM) were determined by UVB-induced HaCaT assay. We assessed protective effects of Saengmaek-san (SM) on LDH release and nitrite release from HaCaT. And COX-2, Bcl-2, Bax, $TNF{\alpha}$, c-jun, c-fos, NF-kB, iNOS, Bcl-xL gene expression were determined in HaCaT using real-time PCR method. Results: 1. SM inhibited LDH-release, nitrite production in UVB-exposed HaCaT. 2. SM suppressed the gene expression of COX-2, $TNF{\alpha}$ in UVB-exposed HaCaT. 3. SM increased the gene expression of Bcl-2, Bax, Bcl-xL family protein in UVB-exposed HaCaT. 4. SM suppressed the gene expression of c-jun, c-fos, NF-kB in UVB-exposed HaCaT. Conclusions: The study showed SM inhibited the cell damage in UVB-exposed HaCaT.

Keywords

References

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