Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
권호 표지

Changes of Gill Structure and Identification of Genes by Muddy Water Exposure in Cyprinus carpio

잉어(Cyprinus carpio)에서 탁수 노출에 의한 아가미 미세구조 변화와 유전자 확인

  • Shin, Myung-Ja (Department of Biological Science, Andong National University) ;
  • Lee, Jong-Eun (Department of Biological Science, Andong National University) ;
  • Seo, Eul-Won (Department of Biological Science, Andong National University)
  • 신명자 (안동대학교 자연과학대학 생명과학과) ;
  • 이종은 (안동대학교 자연과학대학 생명과학과) ;
  • 서을원 (안동대학교 자연과학대학 생명과학과)
  • Received : 2011.02.01
  • Accepted : 2011.03.07
  • Published : 2011.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Present study aimed to investigate morphological change and gene expression in the gill of Cyprinus carpio after exposure against muddy water caused by riverbed disturbance with various rearing condition. The gill of C. carpio showed abnormal shapes in its secondary lamellae and a rough surface with impure debris after exposed to muddy water for 80 days. In addition, the gills showed the edema, the exfoliation of epithelial cell, and the fusion of the secondary lamellae. Using 20 ACPs, 24 differentially expressed genes (DEGs) exposed to muddy water for 80 days were identified. 17 genes among them were up-regulated, while 7 genes were down-regulated in preterm deliveries. A BLAST searches revealed that 3 genes were revealed known genes as calcium transporter 1 (TRPV6) mRNA, macha mRNA for putative puroindoline b protein, and Efnb3 protein-like. Therefore, it is considered that Efnb3 gene from gill would be a useful indicator for neurobehavioral changes in fish influenced by muddy water.

본 연구에서는 하상교란으로 인해 발생한 탁수가 잉어 아가미 조직의 미세구조와 유전적 발현에 미치는 영향을 조사하였다. 탁수에 80일간 사육한 잉어 아가미 조직에서는 이차새변의 간격이 불규칙해지고, 곤봉화, 부종, 상피세포의 박리가 나타났으며, 새변사이에는 이물질의 부착이 두드러지게 관찰되었다. 20개의 ACP를 이용하여 대조군과 탁수에 사육한 잉어 아가미 조직에서 발현차이를 보이는 24개의 밴드를 확인하였고, 그 중 탁수에 사육한 잉어 아가미 조직에서 발현이 증가된 것은 17개, 발현이 감소된 것은 7개였다. BLAST search를 이용하여 확인한 결과 이들 중 각각의 유전자를 대표하는 DNA 절편인 unigene 정보가 있는 유전자는 3개였다. 정보가 있는 유전자 중 발현 증가를 보인 것은 calcium transporter 1 (TRPV6) mRNA, macha mRNA for putative puroindoline b protein 및 Efnb3 protein-like 등이었다. 아가미 조직에서 확인된 Efnb3 유전자는 세포 신호전달 과정에 관여하는 단백질들을 암호화하는 유전자로써 장기간의 탁수는 어류에 스트레스로 작용하여 새로운 유전자 발현에 영향을 줄것으로 사료된다.

Keywords

References

  1. Adams, S.M., M.S. Greeley and M.G. Ryon. 2000. Evaluating effects of contaminants on fish health at multiple levels of biological organization: extrapolating from lower to higher levels. Human and Ecological Risk Assessment 6: 15-27. https://doi.org/10.1080/10807030091124428
  2. Benson, M.D., M.I. Romero, M.E. Lush, Q.R. Lu, M. Henkemeyer and L.F. Parada. 2005. Ephrin-B3 is a myelinbased inhibitor of neurite outgrowth. Proceedings of the National Academy Sciences of the United States of America 102: 10694-10699. https://doi.org/10.1073/pnas.0504021102
  3. Cho, Y.S., S.Y. Lee, C.H. Noh, Y.K. Nam and D.S. Kim. 2006. Survey of genes responsive to long-term heat stress using a cDNA microarray analysis in Mud Loach (Misgurnus mizolepis) liver. Korean Journal of Ichthyology 18: 65-77.
  4. Douglas, S.E., J.W. Gallant, C.E. Bullerwell, C. Wolff, J. Munholland and M.E. Reith. 1999. Winter flounder expressed sequence tags: establishment of an EST database and identification of novel fish genes. Marine Biotechnology 1: 458-464. https://doi.org/10.1007/PL00011802
  5. Ferguson, H.W. 1989. Gill and pseudobranchs, p. 11-40. In: Systematic pathology of fish. Iowa State University Press, Ames.
  6. Kim, I.C., Y.J. Kim, M.K. Kim, Y.D. Yoon and J.S. Lee. 2003. Fish metallothionein gene: amplification with conserved primers and sequence analysis. Korean Journal of Ichthyology 15: 180-185.
  7. Kim, J.H., J.W. Seo, Y.E. Na and K.G. An. 2007. Ecological health assessments on turbidwater in the downstream after a construction of Yongdam Dam. Korean Journal of Limmology 40: 130-142.
  8. Lee, G.S. and G.S. Cho. 2004. Evaluation of GIS-based soil erosion amount with turbid water data. Journal of Korean Society for Geospatial Information System 13: 75-81.
  9. Lee, Y.C., Y.J. Chang and B.K. Lee. 1997. Osmoregulation capability of juvenile grey mullets (Mugil cephalus) with the different salinities. Journal of the Korean Fisheries Society 30: 216-224.
  10. Nam, Y.K. and D.S. Kim. 2001. Bioinformatics in fish: its present status and perspectives with particular emphasis on expressed sequence tags-brief review. Journal of Aquaculture 14: 9-16.
  11. Ogbeibu, A.E. and B.J. Oribhabor. 2002. Ecological impact of river impoundment using benthic macro-invertebrates as indicators. Water Research 36: 2427-2436. https://doi.org/10.1016/S0043-1354(01)00489-4
  12. Seo, J.W. 2005. Fish fauna and ecological characteristics of Dark Chub (Zacco tenminckii) population in the midupper region of Gam Stream. Korean Journal of Limmology 38: 196-206.
  13. Shin, M.J., J.S. Kim, Y.H. Hwang, J.E. Lee and E.W. Seo. 2008. Effect of turbidity changes on tissues of Zacco koreanus. Korean Journal of Limmology 41: 73-80.
  14. Shin, S.W., O.S. Kwon, J.S. Kim, T.S. Chon, S.K. Lee and S.C. Koh. 2000. Tyrosine hydroxylase in Japanese medaka (Oryzias latipes): cDNA cloning and molecular monitoring of TH gene expression As a biomarker. The Korean Journal of Environmental Toxicology 15: 131-137. https://doi.org/10.1002/(SICI)1522-7278(2000)15:2<131::AID-TOX9>3.0.CO;2-Q
  15. Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins. 1997. The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25: 4876-4882. https://doi.org/10.1093/nar/25.24.4876
  16. Yoon, S.J., I.C. Kim, Y.D. Yoon and J.S. Lee. 2003. Assessment of toxic effects in aquatic environment and the fish cytochrome P450 1A (CYP1A) gene. Korean Journal of Environmental Biology 21: 1-7.
  17. Yu, S.H., J.S. Kim, M.J. Shin, J.E. Lee and E.W. Seo. 2009. Effect of turbid water on fishes in the streams of Imha reservoir. Journal of Life Science 19: 1410-1416. https://doi.org/10.5352/JLS.2009.19.10.1410