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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimization of Precipitation Process for the Recovery of Lactic Acid

Lactic acid 회수를 위한 침전공정 최적화

  • Choi, Kook-Hwa (Department of Chemical Engineering, Kongju National University) ;
  • Chang, Yong-Keun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
  • 최국화 (공주대학교 화학공학부) ;
  • 장용근 (한국과학기술원 생명화학공학과) ;
  • 김진현 (공주대학교 화학공학부)
  • Received : 2010.12.07
  • Accepted : 2011.02.14
  • Published : 2011.02.28
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Abstract

In this study, precipitation process was developed for the recovery of the lactic acid from calcium lactate fermentation broth. Calcium lactate yield was improved by decreasing the solubility of calcium lactate through the addition of ethanol (25%, v/v) as a co-precipitant. The optimal lime type, lime concentration, stirrer speed, precipitation time, temperature, and solvent amount for $Ca(LA)_2$ precipitation were CaO, 0.0175 g/mL, 220 rpm, 24 h, $5^{\circ}C$, ethanol 25% (v/v), respectively. Lactic acid was easily and efficiently recovered from precipitated $Ca(LA)_2$ by adding sulfuric acid ($Ca(LA)_2/H_2SO_4$ molar ratio=1:1). In the model solution of organic acids and fermentation broth, the overall yields of recovered lactic acid were 62% and 55%, respectively, under the aforementioned optimal conditions.

본 연구에서는 $Ca(LA)_2$의 침전조건을 최적화하고 침전된 $Ca(LA)_2$ 용액에 황산을 처리하여 고순도, 고수율의 lactic acid를 회수할 수 있는 방법을 개발하고자 하였다. 특히 $Ca(LA)_2$의 용해도를 낮추기 위하여 여러 종류의 유기용매 첨가에 따른 영향을 평가하였다. 모델용액의 경우 침전을 위한 최적의 석회종류, 석회량, 교반속도, 시간, 온도, 유기용매 첨가량은 각각 CaO, 0.0175 g/mL, 220 rpm, 24 h, $5^{\circ}C$, ethanol 25% (v/v)이었으며 최적 조건 하에서 가장 높은 순도 (98%)와 수율(69%)을 얻을 수 있었다. 발효배양액 (lactic acid 순도: 69%, pH: 7.3)의 경우, 모델용액으로부터 얻은 최적의 조건 하에서 $Ca(LA)_2$ 회수 과정에서 64%, 회수된 $Ca(LA)_2$에 황산을 첨가 ($Ca(LA)_2/H_2SO_4$ molar ratio = 1:1)하여 lactic acid를 회수하는 과정에서 86%의 회수율을 각각 얻어 발효배양액으로부터 총괄 수율 55%로 lactic acid (순도: 88.6%)를 얻을 수 있었다.

Keywords

References

  1. Sauer, M., D. Porro, D. Mattanovich, and P. Branduardi (2008) Microbial production of organic acids: expanding the markets. Trends Biotechnol. 26: 100-108. https://doi.org/10.1016/j.tibtech.2007.11.006
  2. Liu, B., M. Yang, B. Qi, X. Chen, Z. Su, and Y. Wan (2010) Optimizing L-(+)-lactic acid production by thermophile Lactobacillus plantarum As.1.3 using alternative nitrogen sources with response surface method. Biochem. Eng. J. 52: 212-219. https://doi.org/10.1016/j.bej.2010.08.013
  3. Cui, F., Y. Li, and C. Wan (2010) Fermentative production of L(+)-lactic acid using hydrolyzed acorn starch, persimmon juice and wheat bran hydrolysate as nutrients. Bioresour. Technol. 101: 3642-3648. https://doi.org/10.1016/j.biortech.2009.12.119
  4. Vaidya, A. N., R. A. Pandey, S. Mudliar, M. S. Kumar, Y. Chakrabarti, and S. Devotta (2005) Production and recovery of lactic acid for polylactide-An overview. Crit. Rev. Environ. Sci. Tech. 35: 429-467. https://doi.org/10.1080/10643380590966181
  5. Lee, E. G., S. H. Kang, H. H. Kim, and Y. K. Chang (2006) Recovery of lactic acid from fermentation broth by the two-stage process of nanofiltration and water-splitting electrodialysis. Biotechnol. Bioprocess Eng. 11: 313-318. https://doi.org/10.1007/BF03026246
  6. Yi, S. S., Y. C. Lu, and G. S. Luo (2008) Separation and concentration of lactic acid by electro-electrodialysis. Sep. Purif. Technol. 60: 308-314. https://doi.org/10.1016/j.seppur.2007.09.004
  7. Ataei, S. A. and E. Vasheghani-Farahani (2008) In situ separation of lactic acid from fermentation broth using ion exchange resins. J. Ind. Microbiol. Biotechnol. 35: 1229-1233. https://doi.org/10.1007/s10295-008-0418-6
  8. Liew, M. K. H., S. Tanaka, and M. Morita (1995) Separation and purification of lactic acid: Fundamental studies on the reverse osmosis down-stream process. Desalination 101: 269-277. https://doi.org/10.1016/0011-9164(95)00030-6
  9. Kaufman, E. N., S. P. Cooper, S. L. Clement, and M. H. Little (1995) Use of a biparticle fluidized-bed bioreactor for continuous and simultaneous fermentation and purification of lactic acid. Appl. Biochem. Biotechnol. 45-46: 605-620.
  10. Park, S. C., S. M. Lee, Y. J. Kim, W. S. Kim, and Y. M. Koo (2006) Recovery of lactic acid from fermentation broth using precipitation and reactive distillation. Korean J. Biotechnol. Bioeng. 21: 199-203.
  11. Jeon, S. I., S. Mun, and J. H. Kim (2006) Optimal temperature control in fractional precipitation for paclitaxel pre-purification. Process Biochem. 41: 276-280. https://doi.org/10.1016/j.procbio.2005.07.016
  12. Mizrahi, J., A. Eyal, C. Riki, B. Hazan, and J. N. Starr (2006) Process for producing a purified lactic acid solution. US Patent 7,026,145 B2.

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