한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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칼슘함량이 강화된 새송이 버섯의 프로테옴 분석

Proteomic Characteristics of Calcium Enriched King Oyster Mushroom (Pleurotus eryngii)

  • 배희선 ((재)포항테크노파크 바이오정보지원센터) ;
  • 김대현 (울산과학기술대학교 나노생명화학공학부) ;
  • 최웅규 (울산과학기술대학교 나노생명화학공학부)
  • Bae, Hee-Sun (Pohang Center for Evaluation of Biomaterials) ;
  • Kim, Dae-Hyun (School of Nano-Bioscience & Chemical Engineering, UNIST) ;
  • Choi, Ung-Kyu (School of Nano-Bioscience & Chemical Engineering, UNIST)
  • 투고 : 2010.09.02
  • 심사 : 2010.12.02
  • 발행 : 2011.02.28
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초록

본 연구에서는 프로테오믹스 기술을 활용하여 칼슘함량이 증가된 새송이버섯과 일반 새송이 버섯에서 단백질 발현의 변화를 조사하였다. 또한 현저한 차이를 보이는 단백질들을 분리, 동정함으로써 새송이버섯 칼슘강화의 기작규명에 기초자료를 제공하고자 하였다. 새송이버섯의 단백질 패턴을 확인한 결과 15 Kda에서 100 Kda 사이에 존재하는 60% 정도의 spot은 산성 pI를 가지며 나머지 40% 정도의 spot은 염기성 영역에 나타났다. 100 Kda 이상에서는 polypeptide spot이 거의 나타나지 않았다. 그리고 9.0 이상의 pI에서도 spot은 거의 나타나지 않았다. 두 배 이상의 발현변화를 보이는 30여개의 spot들 중 10개의 spot에 대한 단백질동정을 할 수 있었다. 10개의 확인된 단백질은 8개의 단백질은 발현이 증가하는 것으로 나타났으며 2개의 단백질은 발현이 감소하는 것으로 나타났다. 동정된 단백질들의 기능을 고찰한 결과 새송이버섯의 칼슘강화기작을 규명하는데 기초자료로 활용할 수 있을 것으로 기대된다.

This study was conducted to identify the differences in proteomic characteristics between Ca-enriched king oyster mushrooms and general king oyster mushrooms. A combined high-throughput proteomic approach was employed to determine the expression profiles and identity of proteins using 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. The overall distribution patterns of the proteins were quite similar, but many of the protein spot intensities varied. A total of 10 proteins, representing a significant difference in the quantities of protein betweenthe two types of mushrooms, were successfully identified. Among these proteins, eight kinds were increased in the Ca-enriched king oyster mushrooms and two kinds were decreased. This study showed that proteomic analysis can help define specific changes in protein level and composition, which can occur in mushrooms where Ca content may or may not be enriched.

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