Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Sound Stress Alters Physiological Processes in Digestion and Immunity and Enhances Insecticide Susceptibility of Spodoptera exigua

스트레스 음파에 따른 파밤나방(Spodoptera exigua ) 소화 및 면역 생리작용 저하와 살충제 감수성 제고 효과

  • Park, Jung-A (School of Bioresource Science, College of Natural Science, Andong National University) ;
  • Seok, Jung-Kyun (School of Bioresource Science, College of Natural Science, Andong National University) ;
  • Prasad, Surakasi Venkara (School of Bioresource Science, College of Natural Science, Andong National University) ;
  • Kim, Yong-Gun (School of Bioresource Science, College of Natural Science, Andong National University)
  • 박정아 (안동대학교 자연과학대학 생명자원과학과) ;
  • 석정균 (안동대학교 자연과학대학 생명자원과학과) ;
  • ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Received : 2011.01.19
  • Accepted : 2011.02.15
  • Published : 2011.03.30
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Abstract

This study analyzed effects of different sound treatments in frequencies and intensities on digestion and immune physiological processes of the beet armyworm, Spodoptera exigua larvae. Without effect on egg hatch, sound treatments with 100-5,000 Hz at 95 dB suppressed feeding behavior and inhibited a digestive enzyme activity. In addition, two dimensional electrophoresis of midgut luminal proteins indicated a marked difference of the sound-treated larvae. In response to 5,000 Hz at 95 dB, larvae showed a significant decrease in hemocyte nodule formation against fungal challenge along with significant suppression in phospholipase $A_2$ activity in hemocyte and plasma. With increase of sound frequencies, the treated larvae showed an enhanced susceptibility to insecticides. Such sound frequency effect was significantly modulated with different sound intensities. These results suggest that sound treatment may give adverse stress to physiological processes of S. exigua larvae and may be applied to a nonchemical insect pest control.

본 연구는 상이한 음파 처리에 따라 파밤나방(Spodoptera exigua) 유충의 소화와 면역 생리반응에 대한 교란 효과를 분석하였다. 조사한 모든 주파수의 음파 처리가 파밤나방 산란에는 뚜렷한 효과를 주지 않았지만, 95 dB 소리세기에서 100-5,000 Hz 주파수의 음파 처리는 유충의 섭식량을 저하시키고, 중장의 소화효소 활성을 억제시켰다. 이 스트레스 음파 처리에 따른 중장 내강에 존재하는 단백질을 이차원전기영동으로 분리하였으며, 무처리구와 상이한 단백질 패턴을 발견하였다. 또한 가장 스트레스 효과를 크게 주는 95 dB의 5,000 Hz 주파수 음파 처리를 받은 유충은 곰팡이 감염에 대해서 혈구의 소낭형성이 억제되었다. 이 음파 처리 받은 유충의 혈구와 혈장에서 인지질분해효소(phospholipase $A_2$: $PLA_2$)의 활성은 현격하게 감소하였다. 스트레스 음파 처리에 따라 파밤나방 유충은 살충제에 대한 감수성이 증가했다. 이러한 상이한 주파수에 따른 생리교란 효과는 소리세기의 차이에 따라 뚜렷하게 차등을 보였다. 이러한 결과는 음파처리가 파밤나방의 생리과정에 스트레스를 주어 비화학적 해충 방제 관리 기술 개발에 적용될 수 있다고 본 연구는 제시한다.

Keywords

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