Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antioxidant Activities and Nitric Oxide Production of Medicine Plants in Gyeongsangbukdo (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, Paeonia lactiflora)

경북약용작물(홍화자, 향부자, 형개, 위유, 작약)의 항산화 및 Nitric Oxide 저해활성

  • Hwang, Eun-Young (Daegu Gyeongbuk Institute for Oriental medicine Industry) ;
  • Kim, Dong-Hee (Daegu Gyeongbuk Institute for Oriental medicine Industry) ;
  • Kim, Hui-Jeong (Daegu Gyeongbuk Institute for Oriental medicine Industry) ;
  • Hwang, Jo-Young (Daegu Gyeongbuk Institute for Oriental medicine Industry) ;
  • Park, Tae-Soon (Daegu Gyeongbuk Institute for Oriental medicine Industry) ;
  • Lee, In-Sun (Department of Food and Technology, Keimyung University) ;
  • Son, Jun-Ho (Daegu Gyeongbuk Institute for Oriental medicine Industry)
  • 황은영 (대구경북한방산업진흥원) ;
  • 김동희 (대구경북한방산업진흥원) ;
  • 김희정 (대구경북한방산업진흥원) ;
  • 황주영 (대구경북한방산업진흥원) ;
  • 박태순 (대구경북한방산업진흥원) ;
  • 이인선 (계명대학교 식품가공학과) ;
  • 손준호 (대구경북한방산업진흥원)
  • Received : 2011.06.07
  • Accepted : 2011.08.25
  • Published : 2011.09.30
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Abstract

This study was carried out to search for natural anti-oxidants and anti-inflammatory compounds from 5 medicinal plants (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, and Paeonia lactiflora). These plants were extracted with 70% ethanol. In order to measure total antioxidant activity of flavonoids, polyphenol content was measured. Radical scavenging activities of extracts were examined using a-a-Diphenyl-${\beta}$-picrylhydrazyl ($DPPH{\cdot}$), 2,2-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid ($ABTS{\cdot}$), ferric reducing antioxidant power (FRAP) and superoxide anion radical assays. C. tinctorius seed extracts showed the highest polyphenol and flavonoid contents as well as strong $DPPH{\cdot}$, $ABTS{\cdot}$, FRAP, and superoxide anion radical scavenging activity. Also, C. tinctorius seed extracts showed the highest nitric oxide (NO) production inhibitory effect. Theses results indicate that the C. tinctorius seed extracts can be used as a functional material due to their effective anti-oxidative and antiinflammatory activities.

본 연구는 5가지 경북약용작물(홍화자, 향부자, 형개, 위유, 작약)의 항산화와 항염증 효과에 대해서 검증하였다. 추출은 70% 에탄올에서 추출하였다. 항산화 활성을 측정하기 위해 총플라보노이드, 폴리페놀 함량을 측정하였고, 추출물의 소거활성은 a-a-Diphenyl-${\beta}$-picrylhydrazyl ($DPPH{\cdot}$), 2,2-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid ($ABTS{\cdot}$), ferric reducing antioxidant power (FRAP), superoxide anion 저해활성으로 측정하였다. 홍화자 추출물에서 높은 총 폴리페놀과 플라보노이드 함량과 뛰어난 $DPPH{\cdot}$, $ABTS{\cdot}$, FRAP, superoxide anion 소거활성을 나타내었다. 또한, 홍화자 추출물에서 가장 높은 NO 생성 저해 활성을 나타냈다. 그 결과 홍화자 추출물은 항산화와 항염증 활성에 효과를 가진 기능성 소재로 사용될 수 있을 것이다.

Keywords

References

  1. Benzie IFF and Strain JJ (1996) The ferric reducing ability of plasma (FRAP) asa measure of "antioxidant power" The FRAP assay. Anal Biochem 239, 70-76. https://doi.org/10.1006/abio.1996.0292
  2. Cha JY, Kim SK, Kim HJ, Somg JY, and Cho YS (2000) Chemical Compositions and Antioxidative Activity of Leek (Allium tuberusum) Seeds. Korean J Life Sci 10, 273-278.
  3. Chance B, Sies H, and Boveris A (1979) Hydroperoxide metabolism in mammalian organs. Physiol Rev 59, 527-605.
  4. Choi JH and Oh SK (1985) Studies on the anti-aging of Korean Ginseng. Korean J Food Sci Technol 17, 506-515.
  5. Choi YM, Kim MH, Shin JJ, Park JM, and Lee JS (2003) The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32, 723-727. https://doi.org/10.3746/jkfn.2003.32.5.723
  6. Davies KJA (1995) Oxidative stress: The paradox of aerobic life. In Free Radicals and Oxidative Stress: Environment, Drugs and Food Additives, Rice-Evans C, Halliwell B, Lunt G (eds.). Biochemical Society Symposium no. 61, pp. 1-31. Portland Press, London, UK.
  7. Ding AH, Nathan CF, and Stuehr DJ (1988) Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparisonof activating cytokines and evidence for independent production. J Immunol 141, 2407-2412.
  8. Folin O and Denis W (1912) On phosphotungastic-phosophomdybdic compounds as color reagents. J Biol Chem 12, 239-249.
  9. Hatano T, Yasuhara T, Yoshihara R, and Okuda T (1991) Inhibitory effects of galloylated flavonoids on xanthine oxidase. Planta Medica 57, 83-86. https://doi.org/10.1055/s-2006-960028
  10. Hwang IW, Lee HR, Kim SK, Zheng HZ, Choi JU, Lee SH, Lee SH, and Chung SK (2008) Proanthocyanidin Content and Antioxidant Characteristics of Grape Seeds. Korean J Food Preserv 15, 859- 863.
  11. Jun MS, Ha YM, Kim, HS, Jang HJ, Kim YM, and Lee YS (2011) Anti-inflammatory action of methanol extract of Carthamus tinstorius involves in heme oxygenase-1 induction. J Ethnophamacol 133, 524-530. https://doi.org/10.1016/j.jep.2010.10.029
  12. Kim HJ, Hwang EY, IM NK, Park SK, and Lee IS (2010) Antioxidant activities of Rumex crispus extracts and effects on quality characteristics of seasoned pork. Korean J Food Sci Technol 42, 445-451.
  13. Kim HJ, Jun BS, Kim SK, Cha JY, and Cho YS (2000) Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and floser of safflower (Carthamus tinctorius L.). J Korean Soc Food Sci Nutr 29, 1127-1132.
  14. Kim JH, Sung NY, Kwon SK, Srinivasan P, Song BS, Choi JI, Yoon Y, Kim JK, Byun MW, Kim MR, and Lee JW (2009) Gammairradiation improves the color and antioxidant properties of Chaga mushroom (Inonotus obliquus) extract. J Med Food 12, 1343- 1347. https://doi.org/10.1089/jmf.2008.1281
  15. Lee ES, Ju HK, Moon TC, Lee E, Jang Y, Lee SH, Son JK, Baek SH, and Chang HW (2004) Inhibition of nitric oxide and tumor necrosis factor-a (TNF-a) production by propenone compound through blockade of nuclear factor (NF)-B activation in cultured murine macrophage. Biol Pharm $B{\mu}LI27$, 617-620. https://doi.org/10.1248/bpb.27.617
  16. Lee KH, Kwon HJ, Chun SS, Kim JH, Cho YJ, and Cha WS (2006) Biological Activities of Extracts from Phellinus linteus. J Korean Soc Appl Biol Chem 49, 298-303.
  17. Lee KS (1997) Content analysis, intake estimation and physiological function of dietary fibers in Korean food. MS thesis, Ewha Womans University, Seoul, Korea.
  18. Lee SE, Seong NS, and Bang JK (2003) Antioxidative activities of korean medicineal plants. Korean J Medicinemal Crop Sci 11, 127-134.
  19. Lee SG, YU MH, Lee SP, and Lee IS (2008) Antiosidant Activities and Induction of Apoptosis by Methanol Extracts from Avocado. J Korean Soc Food Sci Nutr 37, 269-275. https://doi.org/10.3746/jkfn.2008.37.3.269
  20. Lee SO, Lee HJ, Yu MH, Im HG, and Lee IS (2005) Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung Island. Korean J Food Sci Technol 37, 233-240.
  21. Lee YS (2007) Antioxidative and physiological activity of extracts of Angelica dahurica leaves. Korean J Food Preserv 14, 78-86.
  22. Lee YS, Choi BD, and Joo EY (2009) Antioxidative activities and tyrosinase inhibition ability in various extracts of the Vitex rotundifolia seeds. Korean J Food Preserv 16, 101-108.
  23. Miliauskas G, Venskutonis PR, and Been TAV (2004) Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 85, 231-237. https://doi.org/10.1016/j.foodchem.2003.05.007
  24. Miller NJ, Rice-Eans C, Davies MJ, Gopinathan V, and Milner A (1993) A novel method for measuring antioxidant capacty and its application to monitoring the antioxidant suatus in premature neonates. Clin Sci 84, 407-412.
  25. Nivea MMI, Sampietro AR, and Vattuone MA (2000) Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71, 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  26. Parejo I, Viladomat F, Bastida J, Rosas-Romero A, Flerlage N, Burillo J, and Conida C (2002) Comparision between the radical scavenging activity and antioxidant activity of six distilled and nondistilled Mediterranean herbs and aromatic plants. J Agric Food Chem 50, 6882-6890. https://doi.org/10.1021/jf020540a
  27. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, and Riceevans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  28. Rice-Evans CA and Miller NJ (1994) Total antioxidant status in plasma and body fluids. In Methods Enzymol 234, pp. 279-293, Academic Press, California.
  29. Rice-Evans CA, Miller NJ, and Paganga G (1996) Structureantioxidant activity relationships of flavonoids and phenolic acids. Free Rad Biol Med 20, 933-956. https://doi.org/10.1016/0891-5849(95)02227-9
  30. Sim KH and Han YS (2007) The Antimutagenic and Antioxidant Effects of Red Pepper Seed and Red Pepper Pericarp (Capsicum annuum L.). J Food Sci Nutr 12, 273-278. https://doi.org/10.3746/JFN.2007.12.4.273
  31. Singleton VL (1981) Naturally ocurring food toxicants: Phenolic substances of plant origin common in foods. Adv Fd Res 27, 149- 242. https://doi.org/10.1016/S0065-2628(08)60299-2
  32. Song JC, Park NK, Hur HS, Bang MH, and Baek NI (2000) Examination and Isolation of Natural Antioxidants from Korean Medicinal Plants. Korean J Medicinal Crop Sci 8, 94-101.
  33. Storch I and Ferber E (1988) Detergent amplified chemiluminescence of lucigenin for determination of superoxide amini production by NADPH oxidase and xanthine oxidase. Anal Biochem 169, 262- 267. https://doi.org/10.1016/0003-2697(88)90283-7
  34. Yeo SG, Park YB, Kim IS, Kim SB, and Park YH (1995) Inhibition of xanthine oxidase by tea extracts from green tea, oolong tea and black tea. J Korean Soc Food Nutr 24, 154-159.
  35. Yoshizawa S, Horiuchi T, Yoshida T, and Okuda T (1987) $Antit{\mu}Mor$ promoting activity of (-)-epigallocatechin gallate, the main constitutent of tannin in green tea. Phytother Res 1, 44-47. https://doi.org/10.1002/ptr.2650010110
  36. Yu MH, Im HG, Lee HJ, Ji YJ, and Lee IS (2006) Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zizyphus jujuba var. inermis rehder. Korean J Food Sci Technol 38, 128-134.

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