Journal of Haehwa Medicine (혜화의학회지)

Research Institute of Korean Medicine (대전대학교 한의학연구소)
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Effects of Amyda sinensis on Allergic Inflammation Mechanism related Atopy Dermatitis

별갑이 아토피 피부염에서의 알러지성 염증 반응에 미치는 영향

  • Sim, Tae-Kyung (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University) ;
  • Ko, Dae-Kyoung (East-West Comparing Oriental Medicine Society) ;
  • Kim, Hyun-Chang (East-West Comparing Oriental Medicine Society) ;
  • Baek, Yeon-Jong (East-West Comparing Oriental Medicine Society) ;
  • Lee, Jae-Seok (East-West Comparing Oriental Medicine Society) ;
  • Yoo, Hwa-Seung (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University)
  • Received : 2011.06.30
  • Accepted : 2011.08.17
  • Published : 2011.08.20
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Abstract

Objectives: This study aimed to investigate the effects of Amyda sinensis (AS) on allergic inflammation mechanism related atopy dermatitis. Methods: To investigate the effects of AS, We study inhibitory effect of AS on the levels of pro-inflammatory cytokines released from Raw264.7 cell stimulated with LPS (lipopolysaccaride), and EoL-1, THP-1, Jutkat cell stimulated with DP (Dermatophagoides pteronyssinus), and LPS indused acute inflammatory BALB/c mouse model. Result: AS reduced the levels of IL-$1{\beta}$ released from Raw264.7 cell stimulated with LPS at 20 ug/ml, 5 ug/ml concentration, and reduced the levels of IL-6 in a dose-dependent. AS significantly reduced the levels of MCP-1 released from EoL-1 cell stimulated with DP (Dermatophagoides pteronyssinus) at all the concentration, and significantly reduced the level of IL-8 at 0.1 ug/ml concentration. AS significantly reduced the levels of MCP-1 released from THP-1 cell stimulated with DP (Dermatophagoides pteronyssinus) at 1 ug/ml concentration, and reduced the level of IL-6 in a dose-dependent. AS significantly reduced the levels of IL-4 released from Jutkat cell stimulated with DP at all the concentration, and significantly reduced the level of IL-5 at 0.1 ug/ml. 1 ug/ml concentration,. and reduced the level of TNF-${\alpha}$ in a dose-dependent. AS significantly reduced the levels of TNF-${\alpha}$, IL-6, IL-$1{\beta}$, in LPS indused acute inflammatory BALB/c mouse model, in a dose-dependent. Conclusion: These result suggested that AS has suppressive effect on pro-inflammatory cytokines in various cell lines through the regulation of immune system. AS could be applied on the medicinal sources for treatment of immune abnormal diseases such as atopy dermatitis afterward.

Keywords

References

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