한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제19권6호
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  • Pages.484-490
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  • 2011
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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꿀풀 에탄올 추출물 및 분획물의 항산화성

Antioxidant Activity of Different Solvent Fractions from Prunella vulgaris var. lilacina

  • 박동식 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 박미영 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 전상민 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 이진영 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 이영민 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 장환희 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 황경아 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김재현 (농촌진흥청 국립농업과학원 농식품자원부)
  • 투고 : 2011.10.10
  • 심사 : 2011.12.19
  • 발행 : 2011.12.30
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초록

The potential antioxidant activities of different fractions from Prunella vulgaris var. lilacina were assayed in vitro. Among several fractions, n-BuOH fraction showed the highest 1,1-di[henyl-2-picrylhydrazyl (DPPH) free radical scavenging ($IC_{50}=0.50{\mu}g/mL$). The results of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay showed the concentration dependency and n-BuOH fraction appeared a better result than the other fractions at the same concentrati on in this study. Moreover the total phenol and flavonoid contents of n-BuOH fraction contained the highest level. Additionally, correlation analysis indicated a high correlation between the antiradical activity and the total phenolic and flavonoid contents (p < 0.001). It suggests that n-BuOH fraction obtained from the 70% EtOH crude extract of Prunella vulgaris var. lilacina has wide potential for use as a source of antioxidant material.

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참고문헌

  1. Ainsworth EA and Gillespie KM. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature Protocols. 2: 875-877. https://doi.org/10.1038/nprot.2007.102
  2. Alamed J, Chaiyasit W, McClements DJ and Decker EA. (2009). Relationships between free radical scavenging and antioxidant activity in foods. Journal of Agricultural Food Chemistry. 8:2969-2976.
  3. Benzie IF and Strain JJ. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical Biochemistry. 239:70-76. https://doi.org/10.1006/abio.1996.0292
  4. Bors W and Michel C. (2002). Chemistry of the antioxidant effect of polyphenols. Annals of the New York Academy of Sciences. 957: 57-69. https://doi.org/10.1111/j.1749-6632.2002.tb02905.x
  5. Braca A, De Tommasi N, Di Bari L, Pizza C, Politi M and Morelli I. (2001). Antioxidant principles from Bauhinia tarapotensis. Journal of Natural Products. 64: 892-895. https://doi.org/10.1021/np0100845
  6. Buenger J, Ackermann H, Jentzsch A, Mehling A, Pfitzner I, Reiffen KA, Schroeder KR and Wollenweber U. (2006). An interlaboratory comparison of methods used to assess antioxidant potentials. International Journal of Cosmetic Science. 28:135-146. https://doi.org/10.1111/j.1467-2494.2006.00311.x
  7. Choi GP, Chung BH, Lee DI, Lee HY, Lee JH and Kim JD. (2002). Screening of inhibitory activities on angiotensin converting enzyme from medicinal plants. Korean Journal of Medicinal Crop Science. 10:399-402.
  8. Chukwumah Y, Walker LT and Verghese M. (2009). Peanut skin color: a biomarker for total polyphenolic content and antioxidative capacities of peanut cultivars. International Journal of Molecular Sciences. 10:4941-4952. https://doi.org/10.3390/ijms10114941
  9. Cuveler ME, Richard H and Berst C. (1992). Comparison of the antioxidative activity of some acid-phenols: structure-activity relationship. Bioscience, Biotechnology, and Biochemistry. 56:324-325. https://doi.org/10.1271/bbb.56.324
  10. Gate L, Paul J, Ba GN, Tew KD and Tapiero H. (1999). Oxidative stress induced in pathologies: the role of antioxidants. Biomedicine & Pharmacotherapy. 53:169-180. https://doi.org/10.1016/S0753-3322(99)80086-9
  11. Gursoy N, Sihoglu-Tepe A and Tepe B. (2009). Determination of in vitro antioxidative and antimicrobial properties and total phenolic contents of Ziziphora clinopodioides, Cyclotrichium niveum, and Mentha longifolia ssp. typhoides var. typhoides. Journal of Medicinal Food. 12:684-689. https://doi.org/10.1089/jmf.2008.0102
  12. Halliwell B. (1996). Antioxidants in human health and disease. Annual Review of Nutrition. 16:33-50. https://doi.org/10.1146/annurev.nu.16.070196.000341
  13. Jeong CH, Jeong HR, Choi GN, Kim DO, Lee U and Heo HJ. (2011). Neuroprotective and anti-oxidant effects of caffeic acid isolated from Erigeron annuus leaf. Chinese Medicine. 24:25.
  14. Jia Z, Tang M and Wu J. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 64:555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
  15. Lamaison JL, Petitjean-Freytet C and Carnat A. (1991). Medicinal Lamiaceae with antioxidant properties, a potential source of rosmarinic acid. Pharmaceutica Acta Helvetiae. 66:185-188.
  16. Lee JH, Park AR, Choi DW, Kim JD, Kim JC, Ahn JH, Lee HY, Choe M, Choi KP, Shin IC and Park HJ. (2011). Analysis of chemical compositions and electron-donating ability of 4 Korean wild sannamuls. Korean Journal of Medicinal Crop Science. 19:111-116. https://doi.org/10.7783/KJMCS.2011.19.2.111
  17. Mustafa RA, Abdul Hamid A, Mohamed S and Bakar FA. (2010). Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. Journal of Food Science. 75:C28-C35. https://doi.org/10.1111/j.1750-3841.2009.01401.x
  18. Nordberg J and Arner ES. (2001). Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radical Biology & Medicine. 31:1287-1312. https://doi.org/10.1016/S0891-5849(01)00724-9
  19. Park SJ, Kim DH, Lee IK, Jung WY, Park DH, Kim JM, Lee KR, Lee KT, Shin CY, Cheong JH, Ko KH and Ryu JH. (2010). The ameliorating effect of the extract of the flower of Prunella vulgaris var. lilacina on drug-induced memory impairments in mice. Food and Chemical Toxicology. 48:1671-1676. https://doi.org/10.1016/j.fct.2010.03.042
  20. Psotova J, Kolar M, Sousek J, Svagera Z, Vicar J and Ulrichová J. (2003). Biological activities of Prunella vulgaris extract. Phytotheraqpy Research. 17:1082-1087. https://doi.org/10.1002/ptr.1324
  21. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M and Rice-Evans C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine. 26:1231-2137. https://doi.org/10.1016/S0891-5849(98)00315-3
  22. Perez-Fons L, Garzon MT and Micol V. (2010). Relationship between the antioxidant capacity and effect of rosemary (Rosmarinus officinalis L.) polyphenols on membrane phospholipid order. Journal of Agricultural and Food Chemistry. 13:161-171.
  23. Rice-Evans CA, Miller NJ and Paganga G. (1996). Structureantioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology & Medicine. 20:933-956. https://doi.org/10.1016/0891-5849(95)02227-9
  24. Ryu SY, Oak MH, Yoon SK, Cho DI, Yoo GS, Kim TS and Kim KM. (2000) Anti-allergic and anti-inflammatory triterpenes from the herb of Prunella vulgaris. Planta Medica. 66:358-360. https://doi.org/10.1055/s-2000-8531
  25. Seo JS, Lee TH, Lee SM, Lee SE, Seong NS and Kim J. (2009). Inhibitory effects of methanolic extracts of medicinal plants on nitric oxide production in activated marcrophage RAW 264.7 cells. Korean Journal of Medicinal Crop Science. 17:173-178.
  26. Shahidi F and Wanasundara PK. (1992). Phenolic antioxidants. Critical Reviews in Food Science and Nutrition. 32:67-103. https://doi.org/10.1080/10408399209527581
  27. Wang ZJ, Zhao YY, Tu GZ, Hong SL and Chen YY. (1999). Studies on the chemical constituents from Prunella vulgaris. Acta Pharmaceutica Sinica. 34:679-681.
  28. Willcox JK, Ash SL and Catignani GL. (2004). Antioxidants and prevention of chronic disease. Critical Reviews in Food Science and Nutrition. 44:275-295. https://doi.org/10.1080/10408690490468489
  29. Woo HJ, Jun do Y, Lee JY, Woo MH, Yang CH and Kim YH. (2011). Apoptogenic activity of 2,3-dihydroxyurs-12-ene-28-oic acid from Prunella vulgaris var. lilacina is mediated via mitochondria-dependent activation of caspase cascade regulated by Bcl-2 in human acute leukemia Jurkat T cells. Journal of Ethnopharmacology. 135:626-635. https://doi.org/10.1016/j.jep.2011.03.067
  30. Xu HX, Lee SH, Lee SF, White RL and Blay J. (1999). Isolation and characterization of an anti-HSV polysaccharide from Prunella vulgaris. Antiviral Research. 44:43-54. https://doi.org/10.1016/S0166-3542(99)00053-4
  31. Yeum KJ, Aldini G, Chung HY, Krinsky NI and Russell RM. (2003). The activities of antioxidant nutrients in human plasma depend on the localization of attacking radical species. Journal of Nutrition. 133:2688-2691.