Mycobiology

The Korean Society of Mycology (한국균학회)
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Molecular Identification of Two Strains of Phellinus sp. by Internal Transcribed Spacer Sequence Analysis

  • Shin, Kwang-Soo (Department of Microbiology and Biotechnology, Daejeon University)
  • Received : 2011.11.01
  • Accepted : 2011.11.07
  • Published : 2011.12.31
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Abstract

Two species of cultivated Phellinus sp. were identified as P. baumii by internal transcribed spacer (ITS) sequence analysis. The fruit bodies of the examined strains were similar to those of naturally occurring strains, having a bracket-like form, yellow-to-orange color, and poroid hymenial surfaces. The DNA sequences of ITS region of both strains showed a homology of 99% with ITS1 to ITS2 sequences of P. (Inonotus) baumii strain PB0806.

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References

  1. Jeong SC, Cho SP, Yang BK, Jeong YT, Ra KS, Song CH. Immunomodulating activity of the exopolymer from submerged mycelial culture of Phellinus pini. J Microbiol Biotechnol 2004;14:15-21.
  2. Ikekawa J, Nakanishi M, Uehara N, Chihara G, Fukuoka F. Antitumor action of some basidiomycetes, especially Phellinus linteus. Gann 1968;59:155-7.
  3. Lee JH, Cho SM, Kim HM, Hong ND, Yoo ID. Immunostimulating activity of polysaccharides from mycelia of Phellinus linteus grown under different culture conditions. J Microbiol Biotechnol 1996;6:52-5.
  4. Song KS, Cho SM, Lee JH, Kim HM, Han SB, Ko KS, Yoo ID. B-lymphocyte-stimulating polysaccharide from mushroom Phellinus linteus. Chem Pharm Bull (Tokyo) 1995;43:2105-8. https://doi.org/10.1248/cpb.43.2105
  5. Kim HM, Han SB, Oh GT, Kim YH, Hong DH, Hong ND, Yoo ID. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. Int J Immunopharmacol 1996;18:295-303. https://doi.org/10.1016/0192-0561(96)00028-8
  6. Han SB, Lee CW, Jeon YJ, Hong ND, Yoo ID, Yang KH, Kim HM. The inhibitory effect of polysaccharides isolated from Phellinus linteus on tumor growth and metastasis. Immunopharmacology 1999;41:157-64. https://doi.org/10.1016/S0162-3109(98)00063-0
  7. Walder R, Kalvatchev Z, Garzaro D, Barrios M. Natural products from the tropical rain forest of Venezuela as inhibitors of HIV-1 replication. Acta Cient Venez 1995;46:110-4.
  8. Jang BS, Kim JC, Bae JS, Rhee MH, Jang KH, Song JC, Kwon OD, Park SC. Extracts of Phellinus gilvus and Phellinus baumii inhibit pulmonary inflammation induced by lipopolysaccharide in rats. Biotechnol Lett 2004;26:31-3. https://doi.org/10.1023/B:BILE.0000009456.63616.32
  9. White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  10. Jeong WJ. Phylogenetic analysis of Phellinus based on internal transcribed spacer and mitochondrial small subunit ribosomal sequences [dissertation]. Seoul: Seoul National University; 1999.
  11. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82. https://doi.org/10.1093/nar/25.24.4876
  12. Swofford DL. PAUP*4.0b: phylogenetic analysis using parsimony (* and other method). Version 4. Sunderland: Sinauer Associates; 2002.
  13. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783-91. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
  14. Hillis DM, Bull JJ. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst Biol 1993;42:182-92. https://doi.org/10.1093/sysbio/42.2.182
  15. Jeong WJ, Lim YW, Lee JS, Jung HS. Phylogeny of Phellinus and related genera inferred from combined data of ITS and mitochondrial SSU rDNA sequences. J Microbiol Biotechnol 2005;15:1028-38.
  16. Noh JR, Lee IK, Ly SY, Yang KJ, Gang GT, Kim YH, Hwang JH, Yun BS, Lee CH. A Phellinus baumii extract reduces obesity in high-fat diet-fed mice and absorption of triglyceride in lipid-loaded mice. J Med Food 2011;14:209-18. https://doi.org/10.1089/jmf.2010.1152
  17. Shon MY, Kim TH, Sung NJ. Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food Chem 2003;82:593-7. https://doi.org/10.1016/S0308-8146(03)00015-3