Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Comparison between the liquid-liquid partition method and modified QuEChERS method for the analysis of pesticide residues in beef fat

소지방 중 잔류농약분석을 위한 액-액분배법과 modified QuEChERS법 비교

  • Kim, Yoen-Joo (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Choi, Yoon-Hwa (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Shin, Bang-Woo (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Jung-Hark (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 김연주 (서울특별시 보건환경연구원) ;
  • 최윤화 (서울특별시 보건환경연구원) ;
  • 신방우 (서울특별시 보건환경연구원) ;
  • 이정학 (서울특별시 보건환경연구원)
  • Received : 2011.09.23
  • Accepted : 2011.12.21
  • Published : 2011.12.30
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Abstract

This article described the comparison of a quick, easy, cheap, effective, rugged and safe (QuEChERS) sample preparation and the classical method established by National Veterinary Research and Quarantine Service (NVRQS) for the determination of pesticide residues in livestock products using GC-tandem mass spectrometry. The classical method by NVRQS used liquid-liquid partioning followed by evaporizing. The modified QuEChERS entailed extraction of 2 g sample with 15 ml acetonitrile containing 1% acetic acid followed by addition of 6 g anhydrous magnesium sulfate and 1.5 g sodium acetate. After centrifugation, 6 ml of the extract underwent a cleanup step (in a technique known as column-based solid phase extraction) using 400 mg each of $C_{18}$ and primary secondary amine sorbents plus 1,200 mg magnesium sulfate. The quantitation of individual pesticides by both methods was based on tissue standard calibration curves with a correlation coefficient in excess of 0.98 for the 24 pesticides. The detection limits by the classical method were ranged 1.3~5.0 ${\mu}g$/kg, with mean recoveries between 76.2% and 114.3% except aldrin (59.3%) and deltamethrin (63.6%). The detection limits by modified QuEChERS were ranged 0.3~6.2 ${\mu}g$/kg, with mean recoveries between 68.0% and 114.3% except dimethipin (152.6%), chlorfenvinphos (138.1%), 4,4-DDT (61.5%), aldrin (60.4%) and chinomethionate (30.3%).

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References

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