Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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Immunohistochemical localization of cyclic AMP-responsive element binding protein (CREB)-binding protein in the pig retina during postnatal development

  • Oh, Han-Seul (Department of Veterinary Anatomy, Veterinary Medical Research Institute, College of Veterinary Medicine, Jeju National University) ;
  • Kim, Hee-Chul (Department of Veterinary Anatomy, Veterinary Medical Research Institute, College of Veterinary Medicine, Jeju National University) ;
  • Ahn, Mee-Jung (Department of Anatomy, Graduate School of Medicine, Jeju National University) ;
  • Jeong, Chan-Woo (Department of Veterinary Anatomy, Veterinary Medical Research Institute, College of Veterinary Medicine, Jeju National University) ;
  • Jeong, Jin-Woo (Department of Veterinary Anatomy, Veterinary Medical Research Institute, College of Veterinary Medicine, Jeju National University) ;
  • Moon, Chang-Jong (Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Tae-Jyun (Department of Veterinary Anatomy, Veterinary Medical Research Institute, College of Veterinary Medicine, Jeju National University)
  • Published : 2011.06.30
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Abstract

This study evaluated the cellular localization of cyclic AMP-responsive element binding protein-binding protein (CBP) expression in pig retinas during postnatal development. Immunohistochemistry and Western blot analysis were performed on retinal tissue from 2-day-old, 5-week-old, and 6-month-old pigs. Western blot analysis detected the expression of CBP in the retinas of 2-day-old piglets and showed that it was significantly decreased in the retinas of 5-week-old and 6-month-old pigs. Immunohistochemically, CBP was intensely immunostained in protein kinase C alpha (PKC${\alpha}$)-positive-bipolar cells, glutamine synthetase-positive M$\ddot{u}$ller cells, and in ganglion cells in 2-day-old piglets. CBP was detected weakly in the inner plexiform, outer nuclear, and rod and cone layers. CBP immunoreactivity in the ganglion cell layer was decreased in the retinas of 5-week-old and 6-month-old pigs, while clear CBP expression detected in the neurite of PKC${\alpha}$-positive bipolar cells in the inner nuclear layer. In addition, CBP immunoreactivity in M$\ddot{u}$ller cells and glial fibrillary acidic protein-positive glial processes was particularly noteworthy in pig retinas, but not in rat retinas. Th e results indicate that CBP is expressed differentially in the retinal neurons and glial cells according to growth and animal species, and may play an important role in homeostasis in M$\ddot{u}$ller cells, neurite extention in bipolar cells, and signal transduction in photoreceptor cells in the porcine retina.

Keywords

References

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