Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Structural Features of Enzymatic Hydrolysate of Porphyran Isolated from Porphyra yezoensis

방사무늬김(Porphyra yezoensis)에서 추출한 Porphyran 효소 분해물의 화학적 결합 특성

  • Park, Jin-Hee (Department of Food Science and Biotechnology, Kunsan National University) ;
  • Koo, Jae-Geun (Department of Food Science and Biotechnology, Kunsan National University)
  • 박진희 (군산대학교 식품생명공학과) ;
  • 구재근 (군산대학교 식품생명공학과)
  • Received : 2011.10.26
  • Accepted : 2011.12.07
  • Published : 2011.12.30
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Abstract

Enzymatic hydrolysate of porphyran from Porphyra yezoensis was prepared by treatment with ${\beta}$-agarase. The hydrolysate was fractioned into molecular sizes of <3, 3-30, and 30-300 kDa using an ultrafiltration membrane. The membrane fractions were further separated into neutral and anionic fractions using Dowex $1{\times}8$ ion exchange chromatography. After hydrolysis of porphyran with ${\beta}$-agarase, 23.2% of the starting porphyran was recovered as a neutral fraction of low-molecular weight (<3 kDa), and 28.9% remained as an enzyme-resistant anionic fraction of high molecular weight (>300 kDa). Desulfation of porphyran and $^{13}C$-NMR analysis of the anionic fraction of low molecular weight (<3 kDa) showed that the anionic fraction has a backbone consisting of 3-linked ${\beta}$-D-galactose units alternating with either 4-linked a-L-galactose 6-sulfate or 3, 6-anhydro-a-L-galactose units. These results indicate that porphryan is a copolymer of two moieties, about 25% of which are composed of neoagarose moieties and 75% as anionic moieties.

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References

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