불소 도포 방법과 적용 횟수에 따른 법랑질의 표면 특성

The Surface Characteristics of Enamel according to Fluoride Application Methods and Frequency

  • 장선옥 (한림성심대학 치위생과) ;
  • 최은미 (경복대학 치위생과) ;
  • 오상환 (건양대학교 치위생학과) ;
  • 강민경 (연세대학교 치과대학 치과생체재료공학교실 및 연구소) ;
  • 김광만 (연세대학교 치과대학 치과생체재료공학교실 및 연구소)
  • Jang, Sun-Ok (Dept. of Dental Hygiene, Hallym College) ;
  • Choi, Eun-Mi (Dept. of Dental Hygiene, Kyungbok College) ;
  • Oh, Sang-Hwan (Dept. of Dental Hygiene, College of Medical Science, Konyang University) ;
  • Kang, Min-Kyung (Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University) ;
  • Kim, Kwang-Man (Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University)
  • 투고 : 2011.03.02
  • 심사 : 2011.04.07
  • 발행 : 2011.04.30

초록

본 연구는 전문가불소도포로 사용되고 있는 1.23% APF겔과 2% NaF 용액의 적용 횟수와 방법을 달리하여 적용시 치아 경조직의 내산성에 영향을 줄 수 있을지 비교분석하였으며, 다음과 같은 결과를 얻었다. 1. 불소도포를 실시한 모든 그룹에서 표면미세경도가 증가하였고(p<0.05), 불소도포의 횟수를 달리하는 경우 2% NaF 용액을 4회 이온 도포한 그룹에서 표면 미세경도값이 가장 높았다. 2. pH-cycling model 적용 후의 표면미세경도의 변화 비교는 모든 Group에서 불소도포 후에 비해 감소하였지만 불소 적용을 하지 않은 Control group은 가장 낮은 표면미세경도값을 나타내었으며, Control group과 전문가불소도포를 한 Group간에 표면미세경도값은 유의한 차이를 나타내었다(p<0.05). 3. 불소도포 하기 전 Baseline에 비해 pH-cycling model 적용 후 표면미세경도 값은 Control group에서 가장 많은 소실 값을 나타내었고, NaF 용액을 이용하여 4회 이온도포한 Group이 가장 적은 표면미세경도 소실값을 나타내었으며, 유의한 차이를 보였다(p<0.05). 4. Control group에 비해 1회씩 불소 도포한 Group에서 법랑질의 탈회가 적게 관찰되었고, 4회 불소 도포한 Group의 표면은 Control group과 1회씩 불소 도포한 Group에 비해 규칙적인 표면 양상을 보였다. 이상의 실험결과를 종합해보면 법랑질 산부식증 유발환경에서 2% NaF 용액을 4회 이온도포하는 전문가불소도포법이 법랑질 탈회를 최소화할 수 있는 방법으로 나타났다.

The aim of this study was to compare the effects on the resistance to demineralization by the frequency and method of fluoride application in vitro. ninety-one human enamel specimens were embedded in acrylic resin with the labial surfaces exposes. The specimens were divided into 7 groups; (1) non-treated; (2) 1.23% APF gel 1 time; (3) 2% NaF sol 1 time; (4) 2% NaF sol iontophoresis 1 time; (5) 1.23% APF gel 4 time; (6) 2% NaF sol 4 time; (7) 2% NaF sol iontophoresis 4 time. All the groups were immersed in the remineralizing solution (RS) before baseline and divided into 7 test groups of 13 specimens each. All the specimens were exposed to a pH-cycling model which consisted of demineralization (6 hours) and remineralization (18 hours) for 5 days. The Vickers surface micro-hardness number of all the specimens was measured using microhardness tester and the specimen surfaces were observed by scanning electron microscope (SEM). The results were analyzed using one-way ANOVA followed a Tukey's multiple comparison at a significance level of 0.05. The group 7 showed higher level of microhardness after Fluoride application. The group 1 showed lowest level of microhardness but group 7 showed higher level of microhardness after pH-cycling model, there were significant differences between groups. After the modified pH-cycling, the 2% NaF solution with the iontophoresis group showed the best resistance to demineralization(p<0.05). These results were also confirmed by SEM. The fluoride iontophoresis method was the most effective of the regimens in increasing the acid resistance of the enamel.

키워드

참고문헌

  1. Harris No, Garcia-Godoy F: Primary preventive dentistry. 6th ed. New Jersey, Pearson. pp.325-331, 2004.
  2. Loesche WJ: Role of Streptococcus mutans in human dental decay. Microbiological Reviews 50(4): 353-380, 1986.
  3. Banas JA: Virulence properties of Streptococcus mutans. Front Biosci 1(9): 1267-1277, 2004.
  4. Ten Cate JM, Imfeld T: Dental erosion, summary. Eur J Oral Sci 104(2): 241-244, 1996. https://doi.org/10.1111/j.1600-0722.1996.tb00073.x
  5. Attin et al.: Effect of mineral supplements to citric acid on enamel erosion. Arch Oral Biol 48(11): 753-759, 2003. https://doi.org/10.1016/S0003-9969(03)00156-0
  6. Lussi A, Jaeggi T, Jaeggi-Scharer S: Prediction of the erosive potential of some beverages. Caries Res 29(5): 349-354, 1995. https://doi.org/10.1159/000262091
  7. Lussi A, Jaeggi T, Zero DT: The role of diet in the aetiology of dental erosion. Caries Res 38(1): 34-44, 2004. https://doi.org/10.1159/000073918
  8. Zero DT: Etiology of dental erosion--extrinsic factors. Eur J Oral Sci 104(2): 162-177, 1996. https://doi.org/10.1111/j.1600-0722.1996.tb00065.x
  9. Lussi A, Jaeggi T, Schaffner M: Diet and dental erosion. Nutrition 18: 780-781, 2002. https://doi.org/10.1016/S0899-9007(02)00836-5
  10. Imfeld T: Dental erosion: dentition, classification and links. Eur J Oral Sci 104(2): 151-155, 1996. https://doi.org/10.1111/j.1600-0722.1996.tb00063.x
  11. Lussi A et al.: Dental erosion in a population of swiss adults. Community Dent Oral Epidemiol 19(5): 286-290, 1991. https://doi.org/10.1111/j.1600-0528.1991.tb00169.x
  12. Al-Malik MI, Holt RD, Bedi R: Erosion, caries and rampant caries in preschool children in Jeddah, Saudi Arabia. Community Dent Oral Epidemiol 30(1): 16-23, 2002. https://doi.org/10.1034/j.1600-0528.2002.300103.x
  13. Wiegand et al.: Susceptibility of acid-softened enamel to mechanication versus toothbrushing abrasion. Caries Res 41(1): 56-60, 2007. https://doi.org/10.1159/000096106
  14. Imfeld T: Nutrition and dental caries. Non-cariogenic betweenmeal snacks and sweets: a marketplace for small and average-size businesses in the food industry. Swiss Dent 4(9): 6-10, 1983.
  15. Lussi A, Portmann P, Burhop B: Erosion on abraded dental hard tissues by acid lozenges: an in situ study. Clin Oral Investig 1(4): 191-194, 1997.
  16. Lussi A, Schaffner M: Progression of and risk factors for dental erosion and wedge-shaped defects over a 6-year period. Caries Res 34(2): 182-187, 2000. https://doi.org/10.1159/000016587
  17. Linkosalo E, Markkanen H: Dental erosions in relation to lactovegetarian diet. Scand J Dent Res 93(5): 436-441, 1985.
  18. Featherstone JD et al.: Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res 17(5): 385-391, 1983. https://doi.org/10.1159/000260692
  19. Lussi A, Jaeggi T: Erosion-diagnosis and risk factors. Clin Oral Invest. 12(Suppl 1): S5-S13, 2008. https://doi.org/10.1007/s00784-007-0179-z
  20. Hughes et al.: Development and evaluation of a low erosive black currant juice drink. 3. Final drink and concentrate, formulae comparisons in situ and overview of the concept. J Dent. 27(5): 345-350, 1999. https://doi.org/10.1016/S0300-5712(98)00068-2
  21. Bartlett DW, Smith BG, Wilson RF: Comparison of the effect of fluoride and non-fluoride toothpaste on tooth wear in vitro and the influence of enamel fluoride concentration and hardness of enamel. Br Dent J 176(9): 346-348, 1994. https://doi.org/10.1038/sj.bdj.4808450
  22. Larsen MJ, Richards A: The influence of saliva on the formation of calcium fluoride-like material on human dental enamel. Caries Res. 35(1): 57-60, 2001. https://doi.org/10.1159/000047432
  23. Attin T, Buchalla W, Putz B: In vitro evaluation of different remineralization periods in improving the resistance of previously eroded bovine dentine against tooth-brushing abrasion. Arch Oral Biol 46(9): 871-874, 2001. https://doi.org/10.1016/S0003-9969(01)00039-5
  24. Larsen MJ, Richards A: Fluoride is unable to reduce dental erosion from soft drinks. Caries Res 36(1): 75-80, 2002. https://doi.org/10.1159/000057595
  25. Attin T, Deifuss H, Hellwig E: Influence of acidified fluoride gel on abrasion resistance of eroded enamel. Caries Res 33(2): 135-139, 1999. https://doi.org/10.1159/000016507
  26. Ammari JB, Baqain ZH, Ashley PF: Effects of programs for prevention of early childhood caries: a systematic review. Med Princ Pract 16(6): 437-442, 2007. https://doi.org/10.1159/000107748
  27. Issa AI et al.: A study investigating the formation of artificial sub-surface enamel caries-like lesions in deciduous and permanent teeth in the persence and absence of fluoride. Archives of Oral Biology 48(8): 567-571, 2003. https://doi.org/10.1016/S0003-9969(03)00095-5
  28. Brudevold F, McCann HG, Gron P: An enamel biopsy method for determination of fluoride in human teeth. Arch Oral Biol 13(8): 877-885, 1968. https://doi.org/10.1016/0003-9969(68)90003-4
  29. Cruz R, Ogaard B, Rolla G: Acquisition of alkali-soluble fluoride by enamel through treatment with NaF-containing toothpastes in vitro. Scand J Dent Res 100(2): 81-87, 1992.
  30. Yamazaki H, Margolis HC: Enhanced enamel remineralization under acidic conditions in vitro. J Dent Res 87(6): 569-574, 2008. https://doi.org/10.1177/154405910808700612
  31. O'Reilly MM, Featherstone JD: Demineralization and remineralization around orthodontic appliances: an in vivo study. Am J Orthod Dentofacial Orthop 92(1): 33-40, 1987. https://doi.org/10.1016/0889-5406(87)90293-9
  32. Ernest Newburn: Topical fuorides in caries prevention and management: a north American perspective. J Dent Educ 65(10): 1078-1083, 2001.
  33. Singal P, Gupta R, Pandit N: 2% sodium fluoride iontophoresis compared to a commercially abailable desensitizing agent. J. Periodontol 76(3): 351-357, 2005. https://doi.org/10.1902/jop.2005.76.3.351
  34. Simone JL et al.: Iontophoresis: an alternative in the treatment of dental caries? Braz Dent J 6(2): 123-129, 1995.
  35. Wilson JM, Fry BW, Walton RE, Gangarosa LP Sr. Fluoride levels in dentin after iontophoresis of 2% NaF. J Dent Res 63(6): 897-900, 1984. https://doi.org/10.1177/00220345840630061701
  36. 김희은, 권호근, 김백일: 불소이온도입법과 일반불소도포법간의 우치 법랑질에 대한 내산성 비교. 대한구강보건학회지 32(1): 10-19, 2008.
  37. 이영은 등: 시판 불소도포제재들의 법랑질 내산성 증진효과. 대한구강보건학회지 33(1): 19-29, 2009.
  38. Zero DT et al.: Comparison of the iodide permeability test, the surface microhardness test, and mineral dissolution of bovine enamel following acid challenge. Caries Res 24(3): 181-188, 1990. https://doi.org/10.1159/000261263
  39. Sudjalim TR et al.: Prevention of demineralization around orthodontic brackets in vitro. Am J Orthod Dentofacial Orthop 131(6): 705.e1-9, 2007. https://doi.org/10.1016/j.ajodo.2006.09.043
  40. Takagi S et al.: Effect of tooth-bound fluoride on enamel demineralization remineralization in vitro. Caries Research 34: 281-288, 2000. https://doi.org/10.1159/000016603
  41. White DJ, Featherstone JD: A longitudinal microhardness analysis of fluoride dentifrice effects on lesion progression in vitro. Caries Res 21(6): 502-512, 1987. https://doi.org/10.1159/000261059
  42. Jaeggi T, Lussi A: Toothbrush abrasion of erosively altered enamel after intraoral exposure to saliva: an in situ study. Caries Res 33(6): 455-461, 1999. https://doi.org/10.1159/000016551
  43. Zero DT, Lussi A: Erosion--chemical and biological factors of importance to the dental practitioner. Int Dent J 55(4 Suppl 1): 285-290, 2005. https://doi.org/10.1111/j.1875-595X.2005.tb00066.x
  44. Devlin H, Bassiouny MA, Boston D: Hardness of enamel exposed to Coca-Cola and artificial saliva. J Oral Rehabil 33(1): 26-30, 2006. https://doi.org/10.1111/j.1365-2842.2006.01533.x
  45. Wongkhantee S et al.: Effect of acidic food and drinks on surface hardness of enamel, dentine, and tooth-coloured filling materials. J Dent 34(3): 214-220, 2006. https://doi.org/10.1016/j.jdent.2005.06.003
  46. Lussi A, Jaggi T, Scharer S: The influence of different factors on in vitro enamel erosion. Caries Res 27(5): 387-393, 1993. https://doi.org/10.1159/000261569
  47. Meurman JH et al.: Application of a new mechanical properties microprobe to study hardness of eroded bovine enamel in vitro. Scand J Dent Res. 98(6): 568-570, 1990.
  48. Arends J, Ruben JL, Inaba D: Major topics in quantitative microradiography of enamel and dentin: r parameter, mineral distribution visualization, and hyper-remineralization. Adv Dent Res 11(4): 403-414, 1997. https://doi.org/10.1177/08959374970110040501
  49. Wefel JS, Harless JD: The use of saturated DCPD in remineralization of artificial caries lesions in vitro. J Dent Res 66(11): 1640-1643, 1987. https://doi.org/10.1177/00220345870660110701
  50. White DJ, Chen WC, Nancollas GH: Kinetic and physical aspects of enamel remineralization-a constant composition study. Caries Res 22(1): 11-19, 1988. https://doi.org/10.1159/000261077
  51. 이영수 등: 불화나트륨 전기이온도포법과 산성불화인산나트륨겔 도포법의 법랑질 불소이온농도 비교. 대한구강보건학회지 32(1): 31-41, 2008.
  52. Huang GF, Guo MK: Changes of dentinal tubules following fluoride iontophoresis. Proc Natl Sci Counc Repub China B 19(4): 246-252, 1995.
  53. 김민영, 이혜진: 칼슘보강음료가 법랑질 재광화 효과에 미치는 영향. 한국치위생학회지 8(3): 13-22, 2008.