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Inorganic sulfur reduces the motility and invasion of MDA-MB-231 human breast cancer cells

  • Kim, Jin-Joo (Department of Food Science and Nutrition, Dankook University) ;
  • Ha, Hwa-Ae (Department of Food Science and Nutrition, Dankook University) ;
  • Kim, Hee-Sun (Department of Food and Nutrition, Beawha Women's University) ;
  • Kim, Woo-Kyoung (Department of Food Science and Nutrition, Dankook University)
  • Received : 2010.12.15
  • Accepted : 2011.06.29
  • Published : 2011.10.31

Abstract

This study investigated the effects of inorganic sulfur on metastasis in MDA-MB-231 human breast cancer cells. MDA-MB-231 cells were cultured in the absence or presence of various concentrations (12.5, 25, or 50 ${\mu}mol$/L) of inorganic sulfur. Cell motility, invasion, and the activity and mRNA expression of matrix metalloproteases (MMPs) were examined. Numbers of viable MDA-MB-231 cells did not differ by inorganic sulfur treatment from 0 to 50 ${\mu}mol$/L within 48 h. Inorganic sulfur significantly decreased cell motility and invasion in the MDA-MB-231 cells in a dose-dependent manner (P<0.05), as determined using a Boyden chamber assay and a Matrigel chamber. The activities of MMP-2 and MMP-9 were significantly reduced by inorganic sulfur in a dose-dependent manner (P<0.05). The inorganic sulfur also significantly inhibited MMP-2 and MMP-9 expression in the cells (P<0.05). These data suggest that inorganic sulfur can suppress cancer cell motility and invasion by inhibiting MMP-2 and MMP-9 activity and gene expression in MDA-MB-231 cells.

Keywords

References

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