Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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Interaction between odontoblast and bio-calcium phosphate cement reinforced with chitosan

치아모세포와 키토산으로 강화된 생체 적합 칼슘인산시멘트와의 상호작용

  • Chun, Byung-Do (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Sung-Won (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Lee, Sung-Tak (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Tae-Hoon (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Lee, Jung-Han (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Gyoo-Cheon (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Kim, Yong-Deok (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Uk-Kyu (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
  • 전병도 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • 김성원 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • 이성탁 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • 김태훈 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • ;
  • 김규천 ;
  • 김용덕 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • 김욱규 (부산대학교 치의학전문대학원 구강악안면외과)
  • Received : 2011.03.03
  • Accepted : 2011.09.29
  • Published : 2011.10.31
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Abstract

Purpose: Calcium phosphate cement (CPC) is one of many useful materials for restoring tooth defects, periodontium and maxillofacial area. Chitosan is a biodegradable material that has been shown to promote the growth and differentiation of osteoblasts in culture. This study examined the interaction between odontoblasts and bio-calcium phosphate cement reinforced with chitosan. Materials and Methods: $5{\times}10^3$ odontoblastic cells were seeded into each well. Various concentrations of bio-calcium phosphate cement reinforced with chitosan (10, 20, 50, 100, 200, 500 ${\mu}g$/ml, 1, 2, 4 mg/ml) were diluted and added to the wells. The well was incubated for 24 h, 48 h and 72 h. After incubation, the number of cells was assessed to determine the cell viability. A cytokinesis-block micronucleus assay and chromosomal aberration test were carried out to estimate the extent of chromosomal abnormalities. Microscopic photographs and RT-PCR were performed to examine the adhesion potential of bio-calcium phosphate cement reinforced with chitosan. Results: Bio-CPC-reinforced chitosan did not show significant cytotoxicity. The number of damaged chromosomes in the cells treated with Bio-CPC-reinforced chitosan was similar to that in the control cells. There was no significant increase in the number of chromosomal aberrations in the Bio-CPC reinforced chitosan exposed cells. Microscopic photographs and RT-PCR confirmed the adhesive potential of bio-CPC reinforced chitosan to odontoblasts. Conclusion: Bio-CPC-reinforced chitosan did not affect the odontoblastic cell viability, and had no significant cytotoxic effect. Bio-CPC-reinforced chitosan showed adhesive potential to odontoblasts. These results are expected form the basis of future studies on the effectiveness of dental restorative materials in Bio-CPC reinforced with chitosan.

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