Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activity and Nitric Oxide Production of Ethanol Extracts from Astragali membranaceus Bunge and A. membranaceus Bunge var mongholicus Hisiao

황기와 몽고황기 추출물의 항산화 활성 및 Nitric Oxide 생성능

  • Lee, Kwang-Jae (Gangwon Agricultural Research and Extention Services) ;
  • Park, Min-Hee (Gangwon Agricultural Research and Extention Services) ;
  • Park, Yu-Hwa (Gangwon Agricultural Research and Extention Services) ;
  • Lim, Sang-Hyun (Gangwon Agricultural Research and Extention Services) ;
  • Kim, Kyung-Hee (Gangwon Agricultural Research and Extention Services) ;
  • Kim, Young-Guk (National Institute of Horticultural & Herbal Science, R.D.A) ;
  • Ahn, Young-Sup (National Institute of Horticultural & Herbal Science, R.D.A) ;
  • Kim, Hee-Yeon (Gangwon Agricultural Research and Extention Services)
  • Received : 2011.10.24
  • Accepted : 2011.12.13
  • Published : 2011.12.31
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Abstract

The effects of ethanol extracts from Astragali membranaceus Bunge (AMB) and A. membranaceus Bunge var mongholicus Hisiao (AMBMH) on antioxidant and nitric oxide (NO) production were evaluated. The total polyphenol contents of AMBMH extracts from two, four, and six-year old roots was 45.3, 71.3, and 78.0 mg/g, respectively. These values and those of total flavonoid content were higher than those of AMB extracts. The DPPH radical scavenging activity was the highest in ethanol extracts from four-year old AMBMH roots. The ABTS radical scavenging activity was also higher than those of AMB in ethanol extracts from four- and six-year old AMBMH roots, but not in two-year old roots. The NO production of ethanol extracts from six-year old AMBMH roots was higher than that of two- and four-year old AMBMH roots. However, there is no significant difference in NO production based on the cultivation period of AMB.

기원식물별, 재배연수별 황기 에탄올 추출물의 항산화활성 및 NO 생성능을 측정한 결과, 총 폴리페놀 함량은 몽고황기 2년근, 4년근 및 6년근이 각각 45.3 mg/g, 71.3 mg/g 및 78.0 mg/g으로 황기에 비해 더 많은 양이 함유되어 있었으며, 총 플라보노이드 함량 또한 총 폴리페놀 함량과 마찬가지로 몽고황기가 더 많은 양의 총 플라보노이드를 함유하고 있었다. 기원식물별 추출물의 항산화활성 측정 결과, 황기에 비해 몽고황기의 DPPH radical 소거활성이 높았으며, 재배 연수별로는 몽고황기 4년근이 가장 활성이 높았고($IC_{50}$=3.54 mg/mL), 기원식물별 $ABTS^+$ radical 소거활성은 2년근을 제외한 4년근 및 6년근에서는 몽고황기의 $ABTS^+$ radical 소거활성이 높았다. 기원식물에 따른 NO 생성능은 재배기간이 동일한 경우 황기에 비해 몽고황기에서 NO 생성능이 더 높았다. 몽고황기는 모든 연근에서 무처리구에 비해 NO 생성량이 많았으며 특히, 6년근 에탄올 추출물의 NO 생성능이 2년근 및 4년근에 비해 높았으나, 황기는 재배연수에 따른 유의적인 차이는 나타나지 않았다.

Keywords

References

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