Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Antioxidant activity of wood vinegar by bioconversion

생물전환에 의한 발효 목초액의 항산화 활성

  • Cho, Young-Ho (Department of Pharmaceutical Engineering, Konyang University) ;
  • Cho, Jae-Soo (Department of Pharmaceutical Engineering, Konyang University) ;
  • Lee, Gye-Won (Department of Pharmaceutical Engineering, Konyang University)
  • Received : 2011.08.23
  • Accepted : 2011.10.06
  • Published : 2011.10.31
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Abstract

Reactive oxygen species (ROS) are reactive and potentially harmful to cells, causing oxidation of lipids, proteins, and DNA. In humans, the deleterious effects of ROS have been linked with aging, carcinogenesis, and atherosclerosis. In order to investigate an antioxidant activity of wood vinegar by bioconversion, we preferentially analyzed the total acidity, acetic acid, pH, and contents of total polyphenols and flavonoids, respectively. Also, we evaluated the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, superoxide anion radicals, hydrogen peroxide radicals, and nitric oxide radicals. The total acidity and amount of acetic acid of wood vinegar after bioconversion were lower than those of wood vinegar before bioconversion, but the pH was higher than that of wood vinegar before bioconversion. The contents of total polyphenols and flavonoids of wood vinegar after bioconversion were 11.17 mg/$m{\ell}$ and 0.42 mg/$m{\ell}$, respectively. The $SC_{50}$ values were in order of superoxide anion radical scavenging activity < DPPH radical scavenging activity < hydrogen peroxide radical scavenging activity < nitric oxide radical scavenging activity. Therefore, these results suggest that wood vinegar by bioconversion can be useful as primary antioxidants for medicines and cosmetics.

활성 산소종은 반응성이 매우 크고 지질, 단백질 및 핵산에 산화를 유발하여 잠재적으로 세포에 매우 해로운 물질이다. 활성 산소종은 또한 인체에 있어서 노화, 발암, 죽상경화증 유발과 같은 해로운 영향을 준다고 알려졌다. 본 연구에서는 총산도, 초산, pH 및 총 폴리페놀과 총 플라보노이드의 함량 등을 분석하였고, 생물전환된 목초액의 항산화 활성을 조사하기 위하여 DPPH, 초과산화물 음이온, 과산화수소, 산화질소 라디칼 소거 활성을 측정하였다. 생물전환된 목초액의 총산도와 초산의 양은 생물전환 전보다 낮았지만, pH는 오히려 높게 나타났다. 생물전환된 목초액의 총 폴리페놀과 총 플라보노이드의 함량은 각각 11.17 mg/$m{\ell}$ 과 0.42 mg/$m{\ell}$였다. 생물전환된 목초액의 각종 라디칼을 50% 소거하는 농도는 초과산화물 라디칼 소거활성 < DPPH 라디칼 소거활성 < 과산화수소 라디칼 소거활성 < 산화질소 라디칼 소거활성 순으로 나타났다. 따라서 목초액을 생물전환할 경우 여러 가지 라디칼에 대한 소거활성을 높여 천연 의약품 및 화장품 소재로 개발 가능성이 있음을 알 수 있다.

Keywords

References

  1. K. M. Lee, G. T. Jeong and D. H. Park, "Study of antimicrobial and DPPH radical scavenger activity of wood vinegar", Korean J. Biotechnol. Bioeng., 19, 5, pp. 381-384, 2004.
  2. F. Z. Lee, S. H. Choi and J. B. Eun, "The nutrite scavenging and electron ability of bamboo smoke distillates made by steel kiln and earth kiln", Korean. J. Food Sci. Technol., 34, pp. 719-724, 2002.
  3. Y. H. Kim, S. K. Kim, K. S. Kim and Y. H. Lee, "Composition of contituents of commercial wood vinegar liquor in korea", J. Korean Soc. Agric. Chem. Biotechnol., 44, pp. 262-268, 2001.
  4. A. Yasuhara and G. Sugiura, "Volatile compounds in pyroligneous liquids from Karamatu and Chisima-sasa", Argric. Biol. Chem., 51, pp. 3049-3060, 1987. https://doi.org/10.1271/bbb1961.51.3049
  5. H. Yoshimura and T. Hayakawa, "Promoting effect of wood vinegar compounds on the mycelial growth of two Basidiomycete", Tran. Myco. Soc. Japan, 34, pp. 141-151, 1993.
  6. B. Y. Hwang, J. H. Cho, Y. M. Chin and S. Yoshihiro, "Component Analysis of Softwood Vinegar", J. Kor. For. En., 20, pp. 28-34, 2001.
  7. K. M. Lee, G. T. Jeong and D. H. Park, "Study of antimicrobial and DPPH radical scavenger activity of wood vinegar", Korean J. Biotechnol. Bioeng., 19, pp. 381-394, 2004.
  8. Y. Mitsuyoshi and U. Genji, "By-products of wood carbonization III", Mokuzai Gakkaishi, 33, pp. 521-529, 1987.
  9. F. Z. Lee, S. H. Choi and J. B. Eun, "The nitrite scavenging and electron ability of bamboo moke distillates made by steel kiln and earth kiln", Korean J. Food Sci. Technol., 34, pp. 719-724, 2002.
  10. Y. H. Kim, S. K. Kim and Y. H. Lee, "Composition of constituents of commercial wood vinegar liquor in korea", J. Korean Soc. Agric. Chem. Biotechnol., 44, pp. 262-268, 2001.
  11. S. P. Kim and C. H. Nam, "Method of Apply oligneous acid extraction and it's refining", KR 10-2007-0011199, 2007.
  12. C. S. Ku, S. P. Mun, S. B. Park and S. D. Kwon, "Physicochemical changes of vinegars obtained from bamboo and wood during long term aging", Mokche Konhak, 30, pp. 74-79, 2002.
  13. M. W. Byun, "A Method for Refining Wood Vinegarand Refined Wood Vinegar", KR 10-2007-0718235, 2007.
  14. A. K. Lee and H. Y. Chung, "Biological activities of a korean traditional rescription, Negyondaebotang", J. Korean Soc. Food Sci. Nutr., 33, pp. 28-33, 2004. https://doi.org/10.3746/jkfn.2004.33.1.028
  15. A. D. Folin and W. Denis, "A colorimetric method for the determination of phenols and phenol derivatives in urine", J. Biol. Chem., 22, pp. 305-308, 1915.
  16. M. I. Moreno, M. I. Isla, A. R. Sampeiettro and M. A. Vatuone, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina". J. Enthnopharmacol., 71, pp. 109-114, 2000. https://doi.org/10.1016/S0378-8741(99)00189-0
  17. M. S. Blois, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp. 199-120, 1958. https://doi.org/10.1038/181199a0
  18. H. Okamura, A. Mimura, Y. Yakou, M. Niwano and Y. Takahara, "Antioxidant activity of tannins and flavonoids in Eucalptus rostrata", Phytochemistry, 33, pp. 557-561, 1993. https://doi.org/10.1016/0031-9422(93)85448-Z
  19. S. W. Park, S. K. Chung and J. C. Park, "Active oxygen scavenging activity of luteolin-7-O-$\beta$-D-glucoside isolated from Humulus japonicus", J. Korean Soc. Food Sci. Nutr., 29, 1, pp. 106-110, 2000.
  20. H. Kato, I. E. Lee, N. V. Chuyen, S. B. Kim and F. Hayase, "Inhibition of nitrosamine formation by nondialyzable melanoidines", Agric. Biol. Chem., 51, pp. 1333-1338, 1987. https://doi.org/10.1271/bbb1961.51.1333
  21. H. J. Kim, B. S. Jun, S. K. Kim, J. Y. Cha and Y. S. Cho, "Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctiorius L.), J Korean Soc. Food Sci. Nutr., 29, pp. 1127-1132, 2000.
  22. H. K. Kim, Y. E. Kim, J. R. Do, Y. C. Lee and B. Y. Lee, "Antioxidative activity and physiological activity of some Korean medicinal plants", Korean J Food Sci., 27, pp. 80-86, 1995.
  23. B. J. An, J. T Lee, S. A. Lee, J. H. Kwak, J. M. Park, J. Y. Lee and J. H. Son, "Antioxidant effects and application as natural ingredient of Korean Sanguisorbae officinalis L.", J Korean Soc. Appl. Biol., 47, pp. 244-250, 2004.
  24. W. F. Petrone, D. K. English, K. Wong and J. M. McCord, "Free radicals and inflammation: superoxidedependent activation of a neutrophil chemotactic factor in plasma", Proc. Natl. Acad. Sci. USA, 77, pp. 1159-1163, 1980. https://doi.org/10.1073/pnas.77.2.1159
  25. T. Finkel and N. J. Holbrook, "Oxidants, oxidative stress and the biology of ageing", Nature, 408, pp. 239-247, 2000. https://doi.org/10.1038/35041687
  26. J. L. Martindale and N. J. Holbrook, "Cellular response to oxidative stress: signaling for suicide and survival", J Cell Physiol., 192, pp. 1-15, 2002. https://doi.org/10.1002/jcp.10119
  27. M. Greenblatt, S. Mirvish and B. T. So, "Nitrosamine studies : induction of lung adenomas by concurrent administration of sodium nitrite and secondary amines in Swiss mice", J National Cancer Institue, 46, pp. 1029-1034, 1971.
  28. J. A. Lim, B. W. Yun and S. H. Beak, "Antioxidative activity and nitrite scavenging of methanol extract from Salvia plebeia R. Br.", Korean Journal of medicinal Crop Science, 15: 183-188, 2007.
  29. F. J. Cui, T. Z. Li, S. J. Lee, S. J. Park, Y. Lim, K. A. Kim, B. J. Chang, J. H. Lee, M. H. Lee and N. H. Choe, "The effects of air-borne particulate matters on the alveolar macrophages for the iNOS expression and nitric oxide with nitrotyrosilated-proteins formation", Tuberculosis and Respiratory Diseases, 60, pp. 426-436, 2006. https://doi.org/10.4046/trd.2006.60.4.426