Toxicological Research

  • 제27권4호
  • /
  • Pages.261-267
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  • 2011
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
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Four-Week Repeated Oral Toxicity Study of AIP1, a Water-soluble Carbohydrate Fraction from Artemisia iwayomogi in Mice

  • Ryu, Sung-Ha (Department of Pharmaceutical Engineering, Inje University) ;
  • Jo, Hae-Ran (Department of Smart Foods and Drugs, Inje University) ;
  • Kim, Ji-Won (Department of Smart Foods and Drugs, Inje University) ;
  • Youn, Hyun-Joo (School of Biological Sciences, Inje University) ;
  • Kim, Kyu-Bong (College of Pharmacy, Inje University)
  • 투고 : 2011.11.03
  • 심사 : 2011.11.15
  • 발행 : 2011.12.01
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초록

Artemisia iwayomogi, a member of the Compositae, is a perennial herb easily found in Korea and used as a traditional medicine to treat liver disease. AIP1, a water-soluble carbohydrate fraction from Artemisia iwayomogi, showed anti-tumor and immuno-modulating activities in animal studies. A subacute toxicological evaluation of AIP1 was performed for 4 weeks in ICR mice. After administration of AIP1 (0, 20, 100, 500 mg/kg/day), the clinical signs, mortalities, body weight changes, hematology, blood clinical biochemistry, urinalysis, organ histopathology, organ weights and gross finding were examined. The results showed that there were no significant differences in body weight changes, food intakes, water consumptions, or organ weights among different dose groups. Also we observed no death and abnormal clinical signs during the experimental period. Between the groups orally treated with AIP1 and the control group, there was no statistical significance in hematological test or serum biochemical values. Histopathological examination showed no abnormal changes in AIP1 groups. These results suggest that no observed adverse effect level (NOAEL) of the oral administration of AIP1 for 4 weeks was considered to be more than 500 mg/kg/day in mice under the condition investigated in current study.

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참고문헌

  1. Ahn, H., Kim, J.Y., Lee, H.J., Kim, Y.K. and Ryu, J.H. (2003). Inhibitors of inducible nitric oxide synthase expression from Artemisia iwayomogi. Arch. Pharm. Res., 26, 301-305. https://doi.org/10.1007/BF02976959
  2. Hwang, J.S., Ji, H.J., Koo, K.A., Lee, N.H., Yeo, H.K., Cheong, S.W., Park, J.H., Oh, G.S., Yoon, C.S. and Youn, H.J. (2005). AIP1, a water-soluble fraction from Artemisia iwayomogi, suppresses thymocyte apoptosis in vitro and down-regulates the expression of Fas gene. Biol. Pharm. Bull., 28, 921-924. https://doi.org/10.1248/bpb.28.921
  3. Ji, H.J., Yeo, H.K., Lee, N.H., Hwang, J.S., Koo, K.A., Cheong, S.W., Park, J.H., Oh, G.S., Yoon, C.S. and Youn. H.J. (2005). A carbohydrate fraction, AIP1, from Artemisia iwayomogi downregulates Fas gene expression and suppresses apoptotic death of the thymocytes induced by 2,3,7,8-tectrachlorodibenzo-pdioxin. Biotechnol. Lett., 27, 253-257. https://doi.org/10.1007/s10529-004-8294-2
  4. Kang, R.L., Zee, O.P., Kwak, J.H., Kim, Y.S., Park, H.K., Koo, K.A. and Youn, H.J. (1993). The polysaccharide fractions of Artemisia species. Kor. J. Pharmacon., 24, 289-295.
  5. Kim, A.R., Zou, Y.N., Park, T.H., Shim, K.H., Kim, M.S., Kim, N.D., Kim, J.D., Bae, S.J., Choi, J.S. and Chung, H.Y. (2004). Active components from Artemisia iwayomogi displaying ONOO(-) scavenging activity. Phytother. Res., 18, 1-7. https://doi.org/10.1002/ptr.1358
  6. Koo, K.A., Kwak, J.H., Lee, K.R., Zee, O.P., Woo, E.R., Park, H.K. and Youn, H.J. (1994). Antitummer and immouno-modulating activies of the polysaccharide fractions from Artemisia iwayomogi selengensis and Artemisia iwayomogi. Arch. Pharm. Res., 17, 371-374. https://doi.org/10.1007/BF02974179
  7. Korea Food and Drug Administration (2009) Toxicity test guideline for Nonclinical Laboratory Studies. Notification of Korea Food and Drug Administration, No. 2009-116.
  8. Lee, J.A., Sung, H.N., Jeon, C.H., Gill, B.C., Oh, G.S., Youn, H.J. and Park, J.H. (2008a). AIP1, a carbohydrate fraction from Artemisia iwayomogi, modulates the functional differentiation of bone marrow-derived dendritic cells. Int. Immunopharmacol., 8, 534-541. https://doi.org/10.1016/j.intimp.2007.12.005
  9. Lee, J.A., Sung, H.N., Jeon, C.H., Gill, B.C., Oh, G.S., Youn, H.J. and Park, J.H. (2008b). A carbohydrate fraction, AIP1 from Artemisia iwayomogi suppresses pulmonary eosinophilia and Th2-type cytokine production in an ovalbumin-induced allergic asthma. Down-regulation of TNF-alpha expression in the lung. Int. Immunopharmacol., 8, 117-125. https://doi.org/10.1016/j.intimp.2007.10.022
  10. Park, E.J., Nan, J.X., Kim, J.Y., Kang, H.C., Choi, J.H., Lee, S.J., Lee, B.H., Kim, S.J., Lee, J.H., Kim, Y.C. and Sohn, D.H. (2000). The ethanol-soluble part of a hot-water extract from Artemisia iwayomogi inhibits liver fibrosis induced by carbon tetrachloride in rats. J. Pharm. Parmacol., 52, 875-881.
  11. Ricciardolo, F.L. (2003). cNOS-iNOS paradigm and arginase in asthma. Trends. Pharmacol. Sci., 24, 560-561; author reply 562-563. https://doi.org/10.1016/j.tips.2003.09.007
  12. Ryu, J.H., Ahn, H., Kim, J.Y. and Kim, Y.K. (2003). Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophages. Phytother. Res., 17, 485-489. https://doi.org/10.1002/ptr.1180
  13. Taylor, K. (1995). A modification of the phenol sulfuric acid method of total sugar determination. Appl. Biochem. Biothchnol., 105, 83-90.

피인용 문헌

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