화훼연구 (FLOWER RESEARCH JOURNAL)

  • 제18권3호
  • /
  • Pages.193-200
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  • 2010
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  • 1225-5009(pISSN)
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  • 2287-772X(eISSN)
한국화훼학회 (The Korean Society for Floricultural Science)
권호 표지

장미 교배 효율 증대를 위한 화분 임성 검정

An Increment of Crossing Efficiency with Consideration of Pollen Viability Analysis in Rose

  • Hwang, Yoon Jung (Department of Horticulture, Kyungpook National University) ;
  • Song, Chang Min (Department of Horticulture, Kyungpook National University) ;
  • Kwon, Min Kyung (Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Kim, Sung Tae (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Won Hee (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Han, Youn Yol (Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Han, Tae Ho (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Lim, Ki Byung (Department of Horticulture, Kyungpook National University)
  • 투고 : 2010.05.31
  • 심사 : 2010.07.05
  • 발행 : 2010.09.30
⟨ 이전 논문 다음 논문 ⟩

초록

현재 재배되고 있는 장미 재배종은 오랜 기간 동안 종간교잡과 종내교잡을 통해 선발되어 온 것으로서 대부분이 이형잡종의 게놈으로 구성되어 있어 발아율이 저조한 편이다. 따라서, 효율적인 교배를 위해서는 화분의 활력 검정이 선행되어야 한다. 이에 본 연구에서는 염색액과 발아배지를 이용하여 7품종의 화분 임성율과 발아율 검정하고 이들 결과를 토대로 화분의 크기, 배수성 및 교배조합과의 상관관계를 알아보고자 실시하였다. 화분 임성율은 '스칼렛미미'가 82%로 가장 높았으며 '피노키오'가 39%로 가장 낮았다. 발아율 관찰 결과 '스칼렛미미'가 41%로 가장 높았으며 '미니로사'가 1%로 가장 낮았다. 화분의 크기를 관찰한 결과 화분은 큰 화분과 작은 화분으로 구분되었으며 화분의 직경은 각각 $41.3-45.4{\mu}m$과 $30.7-37.4{\mu}m$로 관찰되었으며 화분의 크기는 10-40% 정도 차이가 있는 것으로 나타났다. 화분의 크기와 염색체 전체 길이의 합을 비교한 결과 염색체 크기와 화분의 크기는 정의 상관관계가 있음을 알 수 있었다. 교배조합 검정 결과 착과율과 발아율은 화분 발아율, 임성률 및 배수성과 상관관계가 있음을 확인하였다.

Current rose cultivars are all composed of heterozygous genome due to long history of out crossing including interspecific hybridization. It has been adapted by artificial selection and crossing by breeders that mainly based on the crossing with fertile pollen derived from inter- or intra-specific hybridization. Pollen viability and germination ability tests provide valuable information for the designing of parentage for more successful breeding efficacy. In this study, we tested the pollen viability and germination ability in seven rose cultivars to find any relationship among several factors including pollen size, ploidy levels, and crossing compatibility. The pollen viability showed wide ranges from 39% 'Pinocchio' as minimum to 82% 'Scarlet Mimi' as maximum, whereas pollen germination rate were from 1% 'Mini Rosa' to 41% 'Scarlet Mimi' as a highest. Pollen size ranged from 41.3 to $45.4{\mu}m$ in large sized pollen and 30.7 to $37.4{\mu}m$ in small sized pollen. The mean diameter of large sized pollen is approximately 10-40% bigger than that of small sized pollen. There are positive relationships among ploidy level, total chromosome length, and pollen size. Crossing list showed that seed setting ratio and seed germination were related to pollen viability, pollen germination, and ploidy level.

키워드

과제정보

연구 과제 주관 기관 : 농촌진흥청

참고문헌

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