Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

BONE REGENERATION WITH ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELL AND HA/TCP

HA/TCP 골이식재상에 이식된 지방유래 줄기세포의 골모세포로의 분화 및 골형성에 대한 연구

  • Rim, Jae-Suk (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Gwon, Jong-Jin (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Jang, Hyon-Seok (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Lee, Eui-Seok (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Jeong, You-Min (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Lee, Tai-Hyung (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Park, Jeong-Kyun (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University)
  • 임재석 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 권종진 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 장현석 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 이의석 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 정유민 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 이태형 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 박정균 (고려대학교 의과대학 구강악안면외과학교실)
  • Received : 2009.11.12
  • Accepted : 2010.02.10
  • Published : 2010.03.31
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Abstract

Aim of the study: An alternative source of adult stem cells that could be obtained in large quantities, under local anesthesia, with minimal discomfort would be advantageous. Adipose tissue could be processed to obtain a fibroblast-like population of cells or adipose tissue-derived stromal cells (ATSCs). This study was performed to confirm the availability of ATSCs in bone tissue engineering. Materials amp; Methods: In this study, adipose tissue-derived mesenchymal stem cell was extracted from the liposuctioned abdominal fat of 24-old human and cultivated, and the stem cell surface markers of CD 105 and SCF-R were confirmed by immunofluorescent staining. The proliferation of bone marrow mesenchymal stem cell and ATSCs were compared, and evaluated the osteogenic differentiation of ATSCs in a specific osteogenic induction medium. Osteogenic differentiation was assessed by von Kossa and alkaline phosphatase staining. Expression of osteocyte specific BMP-2, ALP, Cbfa-1, Osteopontin and osteocalcin were confirmed by RT-PCR. With differentiation of ATSCs, calcium concentration was assayed, and osteocalcin was evaluated by ELISA (Enzyme-linked immunosorbant assay). The bone formation by 5-week implantation of HA/TCP block loaded with bone marrow mesenchymal stem cells and ATSCs in the subcutaneous pocket of nude mouse was evaluated by histologic analysis. Results: ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. Expression of osteocyte specific genes was also detected. ATSCs could be easily identified through fluorescence microscopy, and bone formation in vivo was confirmed by using ATSC-loaded HA/TCP scaffold. Conclusions: The present results show that ATSCs have an ability to differentiate into osteoblasts and formed bone in vitro and in vivo. So ATSCs may be an ideal source for further experiments on stem cell biology and bone tissue engineering.

Keywords

References

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