Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Fine Structure of the Epithelial Apoptosis in the Anuran Tadpole Rana nigromaculata

참개구리(Rana nigromaculata) 유생기 상피 세포사의 미세구조

  • Lee, Hye-Won (Department of Biological Sciences, Dankook University) ;
  • Moon, Myung-Jin (Department of Biological Sciences, Dankook University)
  • 이혜원 (단국대학교 생명과학과) ;
  • 문명진 (단국대학교 생명과학과)
  • Received : 2010.05.24
  • Accepted : 2010.06.18
  • Published : 2010.06.30
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Abstract

The fine structural characteristics of the apoptotic cells in the cutaneous epithelium of the anuran tadpole of the black-spotted frog, Rana nigromaculata was examined using the TUNEL (Terminal deoxynucleotidyl transferase-mediated biotinylated d-Uridine triphosphate Nick End Labeling) staining technique and TEM (transmission electron microscopy) observations. The cutaneous epithelium of the tadpole was composed of stratified cuboidal cells and the apoptotic cell death was observed continuously during the tail degeneration stages from the Shumway stage number 31 to 33. The early apoptotic cells shown in the epithelium demonstrated condensation and margination of the chromatin material at the nuclear periphery, and nuclear breakdown and cytoplasmic condensation were followed. Subsequent cytoplasmic degeneration of the apoptotic cell were produced by membrane-bounded cell fragments with relatively well preserved organelles. Following the processes of autophagic degradation, the late apoptotic cells being phagocytosed by other surrounding cells. These nearby cells, presumptive intraepithelial macrophages, contain a variety of lysosomal residual bodies which fuses with other cell organelles or other cytoplasmatic material to form secondary lysosomes. They are soon transformed into lamellar shaped vesicles and finally disappeared during the process of degradation.

변태중인 참개구리(Rana nigromaculata) 유생의 상피에서 세포자멸사가 초래된 세포를 확인하고 이들의 핵과 세포질에서 나타나는 퇴행과정의 미세구조 변화를 TUNEL 염색법과 투과전자현미경 관찰을 통해 분석하였다. 꼬리의 퇴화가 진행되는 Shumway 발생단계 31기부터 33기까지의 유생 상피는 다층의 입방형세포로 이루어져 있었고, TUNEL 염색으로 관찰한 결과, 상피 전역에 걸쳐 광범위한 세포자멸사가 진행되고 있음을 확인할 수 있었다. 자멸사에 의해 초래되는 상피세포의 초기 변형은 먼저 핵에서 관찰되었는데, 염색질이 응축되어 핵막 주변부로 편재되었고 핵의 분절과 세포질의 응축, 그리고 세포막의 변형이 뒤따르는 것으로 확인되었다. 이어서 세포질의 분절에 의해 자멸소체가 형성되었는데 막으로 둘러싸인 소체 내부에서는 비교적 잘 보존된 형태의 세포소기관들이 관찰되었다. 자멸사가 초래된 세포들은 상피내 대식세포(intraepithelial macrophage)로 추정되는 인접 세포들에 의해 탐식되어 잔여소체를 형성하였으며, 급속한 퇴화과정을 거쳐 층판구조를 지닌 소포의 형태로 변형된 후 세포질에서 소멸됨이 확인되었다.

Keywords

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