The Oogenesis of Kribensis, Pelvicachromis pulcher, Cichlidae, Teleostei

경골어류 시클리드과 크리벤시스(Pelvicachromis pulcher)의 난자형성과정

  • Kim, Dong-Heui (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Chang, Byung-Soo (Department of Cosmetology, Hanseo University) ;
  • Teng, Yung-Chien (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Kwon, Jung-Kyun (Department of Electron Microscopy Laboratory, College of Medicine, Hanyang University) ;
  • Lee, Myeong-Seon (Department of Genetic Engineering, Cheongju University) ;
  • Lee, Gui-Young (Department of Beauty Coordination, Keukdong College) ;
  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University)
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 장병수 (한서대학교 보건학부 피부미용학과) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 권중균 (한양대학교 의과대학 전자현미경실) ;
  • 이명선 (청주대학교 이공대학 유전공학과) ;
  • 이귀영 (극동정보대학 뷰티코디네이션학과) ;
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소)
  • Received : 2010.04.20
  • Accepted : 2010.06.14
  • Published : 2010.06.30

Abstract

Kribensis, Pelvicachromis pulcher is a teleost belonging to Cichlidae. The oogenesis was investigated by light microscope. The ovary was located between intestine and air bladder, a yellowish and ellipsoidal shape with the major axis 20mm and the minor axis 5 mm. Cytoplasm of oogonia in early stage was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The basophilic substance of cytoplasm was changed to acidic. Some yolk vesicles started forming small yolk mass except the surrounding nucleus. In case of matured egg, size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. The yolk mass contained crystal-like structures. In conclusion, the oogenesis of Pelvicachromis pulcher was summarized by the increase in cell size, the formation and the accumulation of yolk, and the decrease of basophilic substance in the cytoplasm. The oogenesis of Coreoleuciscus splendidus is similar with other teleost. But there were differences in distribution of yolk vesicle and yolk mass containing cristal-like structures.

경골어류, 시클리드과(Cichlidae)에 속하는 크리벤시스(Pelvicachromis pulcher)의 난자형성과정을 광학현미경으로 관찰한 결과는 다음과 같다. 크리벤시스의 난소는 한 쌍으로 부레와 창자사이에 위치하고 있었으며 황색을 띠었다. 난소의 형태는 장타원형(장축 20 mm, 단축 5 mm)이었다. 초기난원세포는 진한 파란색으로 염색되었으며 발달함에 따라서 난원세포의 세포질은 hematoxylin으로 초기의 난원세포에 비하여 파란색으로 낮게 염색되었으며 핵 안에 여러 개의 인들이 초기시기의 난원세포와 같이 핵막 안쪽에만 배열하는 특징을 보였다 발달함에 따라서 제1난모세포(primary oocyte)는 커졌으며 초기 난원세포에 비하여 색상은 좀 흐려져 보라색에 가깝게 관찰되었고 난막은 관찰되지 않았다. 난황포(yolk vesicle)들은 핵 주변부에는 관찰되지 않았고 세포질의 가장자리에만 국한적으로 형성되기 시작하였다. 제1난모세포가 발달함에 따라서 난황포는 바깥 가장자리에서 안쪽으로 증식되어 핵 쪽으로 이동되었으며 제2난모세포는 세포질이 전체적으로 호산성이었으며 난황포가 세포질 전체에 분포하였다. 일부 난황포는 작은 난황괴를 형성하기 시작하였으나 핵 주변부에서는 난황괴 형성은 많이 이루어지지 않았다. 성숙한 난자는 난자의 크기가 상당히 커졌으며 난황포는 더욱 발달하여 난황괴(yolk mass)를 이루고 있었고 성숙난의 난황괴를 확대한 결과 난황괴속에 염색밀도가 낮은 다각형 또는 타원형의 결정구조물들이 채워져 있었다. 이상과 같이 크리벤시스의 난자형성과정은 난세포의 크기증가, 난황낭의 축적, 세포질 내 염기성 물질이 산성으로 전환 및 난막의 발달로 요약될 수 있으며 일반적인 다른 과의 담수경골어류와 큰 차이는 없는 것으로 확인되었다. 그러나 난황포의 배열방식과 난황괴의 형태는 다른 종과 구별되는 종특이성이다.

Keywords

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