Survey of Corneal Thickness, Endothelial Cell and Intraocular Pressure in New Zealand White Rabbits

뉴질랜드 흰 토끼의 각막 두께, 내피세포 및 안압에 관한 조사

  • Chang, Hwa-Seok (Department of Ophthalmology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Lee, Dae-Yeoup (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University) ;
  • Kang, Eun-Hee (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University) ;
  • Lee, Jae-Hoon (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University) ;
  • Cho, Yang-Kyeung (Department of Ophthalmology, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Kim, Man-Soo (Department of Ophthalmology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Kim, Hwi-Yool (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University)
  • 장화석 (가톨릭대학교 의과대학 서울성모병원) ;
  • 이대엽 (건국대학교 수의과대학) ;
  • 강은희 (건국대학교 수의과대학) ;
  • 이재훈 (건국대학교 수의과대학) ;
  • 조양경 (가톨릭대학교 의과대학 성빈센트병원) ;
  • 김만수 (가톨릭대학교 의과대학 서울성모병원) ;
  • 김휘율 (건국대학교 수의과대학)
  • Accepted : 2010.02.09
  • Published : 2010.08.30

Abstract

Rabbit's cornea is the most common thing that used for researches in ophthalmology, so many experimentations have done. The purpose of this study was to survey the corneal pachymetry, tonometry and specular microscopy in New Zealand white rabbits. Three months old male rabbits ($2.9{\pm}0.2\;kg$) were examined with contact the specular microscope to evaluate the endothelial cell density, coefficiency of variation and the percentage of hexagonal cells in the central cornea. For the pachymetry, ultrasonic pachymeter was used for central cornea in each twenty rabbits, and tonometry was performed forty rabbit's eyes to evaluate the intraocular pressure. Corneal endothelial cell density in New Zealand white rabbit was $3304{\pm}354\;cell/mm^2$. Coefficiency of variation was $45.8{\pm}2$ and the percentage of hexagonal cells in the central cornea was $44.4{\pm}11%$. The pachymetry of cornea was $346.1{\pm}21\;{\mu}m$ and tonometry of eye was $14.3{\pm}2\;mmHg$. It is considered that normal New Zealand white rabbit's pachymetry, specular microscopy and tonometry were useful basic data of rabbit's cornea for research and clinic in veterinary ophthalmology.

Keywords

References

  1. Brugin E, Ghirlando A, Gambato C, Midena E. Central corneal thickness: z-ring corneal confocal microscopy versus ultrasound pachymetry. Cornea 2007; 26: 303-307. https://doi.org/10.1097/ICO.0b013e31802e1dea
  2. Diaz-valle D, Sandchez JMBC, Castillo A, Sayagues O, Moriche M. Endothelial damage with cataract surgery techniques. J. Cataract of Refract. Surg. 1998; 24: 951-955. https://doi.org/10.1016/S0886-3350(98)80049-7
  3. Gilger BC, Wright JC, Whitley RD, McLaughlin SA. Corneal thickness measured by ultrasonic pachyemetry in cats. Am J Vet Res 1993; 52: 228-230.
  4. Gilger BC, Whitley RD, McLaughin SA, Wright JC and Drane JW. Canine corneal thickness measured by ultrasonic pachymetry. Am J Vet Res 1991; 52: 1570-1572.
  5. Gwin RM, Lerner I, Warren JK, Gum G. Decrease in canine corneal endothelial cell density and increase in corneal thickness as functions of age. Invest Ophthalmol Vis Sci 1982; 22: 267-271.
  6. Jeong BW, Park CK, Han HJ. Endothelial cell density changes in the normal Korean cornea during life. J Kor Ophthal Soci 1988; 29: 27-32.
  7. Jeong MB, Kim NR, Yi NY, Park SA, Kim MS, Park JH, Jeong SM, Seo KD, Nam TC, Oh Ys, Won MW, Seo KM. Spontaneous ophthalmic diseases in 586 New Zealand White Rabbits. Exp Anim 2005; 54; 395-403. https://doi.org/10.1538/expanim.54.395
  8. Kim BK, Yun YM, Seong JK, Seo KM. Spontaneous ophthalmic diseases of Beagles in Korea. J Vet Sci 2001; 41: 113-121.
  9. Kim KS, Park SY, Oh JS. Morphometric analysis of the corneal endothelial cells in normal Korean. J Kor Ophthal Soci 1992; 33: 24-29.
  10. Kim WH, Kweon OK. Diurnal change of intraocular pressure and comparison of Tono-penXL and Schiotz Tonometer in normal dogs. J Vet Clin 2002; 19: 415-417.
  11. Labbe A, Liang H, Martin C, Brignole-Baudouin F, Warnet JM, Baudouin C. Comparative anatomy of laboratory animal corneas with a new-generation high-resolution in vivo confocal microscope. Curr Eye Res 2006; 31: 501-509. https://doi.org/10.1080/02713680600701513
  12. Lee SW, Na HK. Change of the corneal endothelial cell density in postoperative cataract extraction cases. J Kor Ophthal Soci 1981; 22: 17-42.
  13. Lee YS. Rabbit. In: Introduction of Lab animal, 1st eds. Seoul: Sharon. 2000: 163-172,
  14. MacCallum DK, Bahn CF, Lillie JH, Meyer RF, Martonyi CL. Evidence for corneal endothelial cell hypertrophy during postnatal growth of the cat cornea. Invest Ophthalmol Vis Sci 1983; 24: 247-250.
  15. Mishima S. Clinical investigation on the corneal endothelium. Am J Ophthalmol 1982; 93: 1-29.
  16. Oh JY, Wee WR, Lee JH, Kim MK. Short-term effect of intracameral triamcinolone acetonide on corneal endothelium using the rabbit model. Eye 2007; 21: 812-818. https://doi.org/10.1038/sj.eye.6702357
  17. Park SO, Cho BJ. Central corneal thickness measured by ultrasonic pachymeter in normal Koreans. J Kor Ophthal Soci 2000; 41: 2332-2336.
  18. Piggatto JAT, Freire CD, Abib FC, Barros PSM, Quinze R, Ortiz JPD, Santos JM, Laus JL. Cell size shape relationships in corneal endothelium of dogs. Vet Oph 2004; 7: 440-453.
  19. Reitsmer HA, Kiel JW, Harrison JM, Ransom NL, McKinnon SJ. Tonopen measurement of intraocular pressure in mice. Exp Eye Res 2005; 80: 295-296. https://doi.org/10.1016/j.exer.2004.11.008
  20. Robert MG, Irving LJ, Kay W, Glenwood G. Decrease canine corneal endothelial cell density and increase in corneal thickness as function of age. Assoc for Res In Vis And Ophthal 1982; 22: 267-271.
  21. Ronald AL, Marita MS, Amado RB, Howard ML. Change in the corneal endothelium as a function of age. Exp Eye Res 1976; 22: 587-594. https://doi.org/10.1016/0014-4835(76)90003-8
  22. Rupert RB. Effect of cataract surgery on the corneal endothelium. Am Oph 2004; 111: 679-685.
  23. Schulz D, Iliev ME, Frueh BE, Goldblum D. In vivo pachymetry in normal eyes of rats, mice and rabbits with the optical low coherence reflectometer. Vision Res 2003; 43: 723-728. https://doi.org/10.1016/S0042-6989(03)00005-1
  24. Szaflik JP, Comparison of in vivo confocal microscopy of human cornea by white light scanning slit and laser scanning systems. Cornea 2007; 26: 438-445. https://doi.org/10.1097/ICO.0b013e31803dcd11