Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Community Structure, Diversity, and Vertical Distribution of Archaea Revealed by 16S rRNA Gene Analysis in the Deep Sea Sediment of the Ulleung Basin, East Sea

16S rRNA 유전자 분석방법을 이용한 동해 울릉분지 심해 퇴적물 내 고세균 군집 구조 및 다양성의 수직분포 특성연구

  • Kim, Bo-Bae (Department of Environmental Marine Sciences Division of Science and Technology Hanyang University) ;
  • Cho, Hye-Youn (Department of Environmental Marine Sciences Division of Science and Technology Hanyang University) ;
  • Hyun, Jung-Ho (Department of Environmental Marine Sciences Division of Science and Technology Hanyang University)
  • 김보배 (한양대학교 과학기술학부 해양환경과학과) ;
  • 조혜연 (한양대학교 과학기술학부 해양환경과학과) ;
  • 현정호 (한양대학교 과학기술학부 해양환경과학과)
  • Received : 2010.08.11
  • Accepted : 2010.09.13
  • Published : 2010.09.30
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Abstract

To assess community structure and diversity of archaea, a clone sequencing analysis based on an archaeal 16S rRNA gene was conducted at three sediment depths of the continental slope and Ulleung Basin in the East Sea. A total of 311 and 342 clones were sequenced at the slope and basin sites, respectively. Marine Group I, which is known as the ammonia oxidizers, appeared to predominate in the surface sediment of both sites (97.3% at slope, 88.5% at basin). In the anoxic subsurface sediment of the slope and basin, the predominant archaeal group differed noticeably. Marine Benthic Group B dominated in the subsurface sediment of the slope. Marine Benthic Group D and Miscellaneous Crenarchaeotal Group were the second largest archaeal group at 8-9 cm and 18-19 cm depth, respectively. Marine Benthic Group C of Crenarchaeota occupied the highest proportion by accounting for more than 60% of total clones in the subsurface sediments of the basin site. While archaeal groups that use metal oxide as an electron acceptor were relatively more abundant at the basin sites with manganese (Mn) oxide-enriched surface sediment, archaeal groups related to the sulfur cycle were more abundant in the sulfidogenic sediments of the slope. Overall results indicate that archaeal communities in the Ulleung Basin show clear spatial variation with depth and sites according to geochemical properties the sediment. Archaeal communities also seem to play a significant role in the biogeochemical carbon (C), nitrogen (N), sulfur (S), and metal cycles at each site.

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