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Evaluation on Red Pepper Germplasm lines (Capsicum spp.) for Resistance to Anthracnose Caused by Colletotrichum acutaum

  • Kim, Jeong-Soon (National Agrobiodiversity Center, National Academy of Agricultural Science and Technology, Rural Development Administration) ;
  • Jee, Hyeong-Jin (Organic Farming Technology Division, National Academy of Agricultural Science and Technology, Rural Development Administration) ;
  • Gwag, Jae-Gyun (National Agrobiodiversity Center, National Academy of Agricultural Science and Technology, Rural Development Administration) ;
  • Kim, Chung-Kon (National Agrobiodiversity Center, National Academy of Agricultural Science and Technology, Rural Development Administration) ;
  • Shim, Chang-Ki (National Agrobiodiversity Center, National Academy of Agricultural Science and Technology, Rural Development Administration)
  • Received : 2010.01.31
  • Accepted : 2010.06.29
  • Published : 2010.09.01

Abstract

We evaluated 209 Capsicum accessions from the National Agrobiodiversity of RDA, for their reaction against of anthracnose disease caused by Colletotrichum acutatum. Two hundred nine accessions of Capsicum were almost composed with Capsicum annuum var. annuum originated from 37 countries. The percentage of infection due to C. acutatum ranged from 11.1% to 100% on immature and matured pepper fruits at 21 days after inoculation. On immature fruits of pepper, one hundred seventy three accessions of tested pepper were found infected with C. acutatum. Out of the 173 accessions, eighty five accessions were susceptible to anthracnose with dark brown to black lesions and slowly increased in size at 21 days after inoculation. Only thirty six accessions showed resistance against C. acutatum. On mature fruits of pepper, one hundred ninety eight accessions were infected with C. acutatum at 21 days after inoculation. Twenty one accessions showed a resistance reaction against C. acutatum. The results of this work indicated that all of resistance accessions to C. acutatum were have a genetic potential for the resources of resistance can be further used in pepper breeding programme against anthracnose and also demand more detailed investigation in this.

Keywords

References

  1. Andrews, J. 1995. Peppers: The domesticated Capsicums, new, ed., University of Texas Press. Austin, USA. 186 p.
  2. Andrews, J. 1999. The Pepper Trail: History and recipes from around the world. University of North Texas Press. Texas, USA. 261 p.
  3. Barla, J. B. and Bosland, P. W. 2004. Unraveling the species dilemma in Capsicum frutescens and C. Chinense (Solanaceae): A multiple evidence approach using morphology. Molecular analysis and sexual compatibility. J. American Soc. Hor. Sci. 129:826-832.
  4. Berstein, B., Zehr, E. I., Dean, R. A. and Shabi, E. 1995. Characteristics of Colletotrichum from peach, apple, pecan, and other hosts. Plant Dis. 79:478-482. https://doi.org/10.1094/PD-79-0478
  5. Bosland, P. W. 1994. Chiles: history, cultivation, and uses. pp. 347-366. In: G. Charalambous, ed., Spices, herbs, and edible fungi. Elsevier Publ., New York.
  6. Bosland, P. W. 1996. Capsicums: Innovative uses of an ancient crop. pp. 479-487. In: J. Janick, ed., Spices, herbs, and edible fungi. Elsevier Publ., New York.
  7. Bosland, P. W. and Votava, E. J. 2000. Peppers: Vegetable and spice Capsicums. Crop Production Science in Horticulture 12. CAB. International Publishing, Wallingford, England, UK. 204p.
  8. Chae, Y., Shieh, J. C., Park, J. B. and Engle, L. M. 2003. Morphological evaluation of Capsicum germplasm using statistic analysis. Kor. J. Hort. Sci. Technol. 21(Suppl. II):63.
  9. Cheema, D. S., Singh, D. P., Eawal, R. D. and Deshpande, A. A. 1984. Inheritance of resistance to anthracnose disease in chillies. Capsicum Newsletter 3, 44 p
  10. Choi, K. S. and Pae, D. H. 1987. Studies on the breeding of resistant varieties of pepper 3. Study on the inoculation methods for resistant varieties of pepper to anthracnose. Rural Dev. Administration (Hort.) 29:16-23.
  11. Collins, M. D., Mayer-Wasmund, L. and Bosland, P. W. 1995. Improved method for quantifying capsaicinoids in Capsicum using high performance liquid chromatography. HortScience 30:137-139.
  12. Cronin, J. R. 2002. The Chili peppers pungent principle: Capsaicin delivers diverse health benefits. Alternative and Complementary Therapies 8:110-113. https://doi.org/10.1089/10762800252909865
  13. Eshbaugh, W. H. 1993. Peppers: History and exploitation of a serendipitous new crop discovery. In J. Jack and J. E. Simon eds, New Crops, pp. 132-139. John Wiley & Sons, New York, USA.
  14. Freeman, S. and Katan, T. 1997. Identification of Colletotrichum species responsible for anthracnose and root necrosis of strawberry in Israel. Phytopathology 87:516-521. https://doi.org/10.1094/PHYTO.1997.87.5.516
  15. Freeman, S., Katan, T. and Shabi, E. 1998. Characteristics of Colletotrichum species responsible for anthracnose disease of various fruits. Plant Dis. 82:596-605. https://doi.org/10.1094/PDIS.1998.82.6.596
  16. Hadden, J. F. and Black, L. L. 1988. Anthracnose of pepper caused by Colletotrichum spp. pp. 189-199. In: Tomato and Pepper production in Tropics. S. K. Green, ed., AVRDC, Shanhua, Taiwan.
  17. Heiser, Jr. C. B. 1976. Peppers: Capsicum (Solanaceae). In: N. W. Simmonds, ed., Evolution of Crop plants, pp. 265-268. Longman, London, UK.
  18. Heiser, Jr. C. B. 1985. Ethnobotany of the naranjilla (Solanum quitoense) and its relatives. Economic Botany 39:4-11 https://doi.org/10.1007/BF02861168
  19. Heiser, Jr. C. B. and Pickersgill, B. 1969. Names for the cultivated Capsicum species (Solanaceae). Taxon 18:277-283. https://doi.org/10.2307/1218828
  20. Heiser, Jr. C. B. and Smith P. C. 1953. The cultivated Capsicum peppers. Eco. Bot. 1:214-227.
  21. Hoffman, P. G., Lego, M. C. and Galetto, W. G. 1983. Separation and quantitation of red pepper major heat principles by reverse-phase high pressure liquid chromatography. J. Agr. Food Chem. 31:1326-1330. https://doi.org/10.1021/jf00120a044
  22. Hong, J. K. and Hwang, B. K. 1998. Influence of inoculums density, wetness duration, plant age, inoculation method, and cultivar resistance on infection of pepper plants by Colletotrichum cocodes. Plant Dis. 82:1079-1083. https://doi.org/10.1094/PDIS.1998.82.10.1079
  23. IBPGR. 1983. Genetic resources of Capsicum. In: Board for plant Genetic Resources, Rome.
  24. Idu, M. and Ogbe, T. M. 1997. Studies on the morphology of Capsicum species. J. Plant Anatomy and Morphology 7:114-123.
  25. Jarret, R. L. and Dang, P. 2004. Revisiting the waxy locus and the Capsicum annuum L. complex. Georgia. J. of Science 62:117-133.
  26. Kim D. H., Jeon, Y. A., Go, S. J., Lee, J. K. and Hong, S. B. 2006. Reidentification of Colletotrichum gloeosporioides and C. acutatum isolates stored in Korean Agricultural Culture Collection (KACC). Res. Plant Dis. 12:168-177. https://doi.org/10.5423/RPD.2006.12.3.168
  27. Kim, C. H. and Park, K. S. 1998. A predictive model of disease progression of red-pepper anthracnose. Korean J. Plant Pathol. 4:325-331.
  28. Kim, H. G. and Nam, M. H. 1999. Anthracnose of strawberry in Korea. Plant Dis. Agric. 5:8-13.
  29. Kim, J. T., Park, S. Y., Choi, W. B., Lee, Y. H. and Kim, H. T. 2008. Characterization of Colletotrichum isolates causing anthracnose of pepper in Korea. Plant Pathol. J. 24:17-23. https://doi.org/10.5423/PPJ.2008.24.1.017
  30. Kim, K. K., Yoon, J. B., Park, H. G., Park, E. W. and Kim, Y. H. 2004. Structural modifications and programmed cell death of chili pepper fruits related to resistance responses to Colletotrichum gloeosporioides infection. Genetics and Resistance. 94:1295-1304.
  31. Kim, S. T., Yoon, J. B., J. W. and Park, H. G. 2008. A major recessive gene associated with anthracnose resistance to Colletotrichum capsici in chili pepper (Capsicum annuum L.). Breeding Science 58:137-141. https://doi.org/10.1270/jsbbs.58.137
  32. Kim, W. G., Cho, E. G. and Lee, E. J. 1986. Two strains of Colletotrichum gloeosporioides Prnz. causing anthracnose of pepper fruits. Kor. J. Plant Pathol. 2:107-113.
  33. Kim, Y. S., Min, J. Y., Kang, B. K., Bach, N. V., Choi, W. B., Park, E. W. and Kim, H. T. 2007. Analysis of the less benzimidazole-sensitivity of the isolates of Colletotrichum spp. causing the anthracnose in Pepper and Strawberry. Plant Pathol. J. 23:187-192. https://doi.org/10.5423/PPJ.2007.23.3.187
  34. Kwon, T. R., Pae, D. H., Shin, Y. A., Oh, D. G. and Lee, J. M. 2006. Capsicum Peppers: A Vital Crop for Korea. Chronica Horticulturae 46:16-19.
  35. Krishna De, A. 2003. Capsicum: The genus Capsicum. Medicinal and aromatic plants-industrial profiles Vo. 33. Taylor & Francis, London and New York, 275 p.
  36. Lin, Q., Kanchana-udomkarn, C., Jaunet, T. and Mongkolporn, O. 2002. Genetic analysis of resistance to pepper anthracnose caused by Colletotrichum capsici. Thai J. Agricultural Sci. 35:259-264.
  37. Manandhar, J. B., Hartman, G. L. and Wang, T. C. 1995. Anthracnose development on pepper fruits inoculated with Colletotrichum gloeosporioides. Plant Dis. 79:380-383. https://doi.org/10.1094/PD-79-0380
  38. Marin, A., Ferreres, F. F., Tomaa Barberan, F. A. and Gil, M. 2004. Characterization and quantitation of antioxidant constituents of sweet pepper (Cpasicum annuum L.). J. Agr. Food Chemistry 52:3861-3869. https://doi.org/10.1021/jf0497915
  39. Ministry of Food, Agriculture, Forestry and Fisheries. 2008. Agricultural and Forestry Statistical Yearbook.
  40. Oh, I. S., In, M. S., Woo, I. S., Lee, S. K. and Yu, S. H. 1988. Anthracnose of Pepper seedling caused by Colletotrichum coccodes (Wallr.) Hughes. Kor. J. Mycol. 16:151-156.
  41. Osuna-Garcia, J. A., Wall, M. W. and Waddell, C. A. 1998. Endogenous levels of tocopherols and ascorbic acid during fruit ripening of New Mexican-typing Chile (Cpasicum annuum L.) cultivars. J. Agr. Food Chemistry 46:5093-5096. https://doi.org/10.1021/jf980588h
  42. Pae, D. H., Chae, Y. T., Wang, C. and Engle, L. M. 2003. Selection of new breeding materials with resistant to anthracnose in Capsicum annuum. Kor. J. Hort. Sci. Technol. 21 (Supp. II): 63.
  43. Pae, K. S., Yoon, J. Y. and Lee, J. M. 1998. Screening for resistance to Colletotrichum gloeosporioides using detached fruits in pepper (Capsicum annuum) by high-pressure spray. J. Kor. Soc. Hort. Sci. 39:690-692.
  44. Pakdeevaraporn, P., Wasee, S., Taylor, P. W. J. and Mongkolporn, O. 2005. Inheritance of resistance to anthracnose caused by Colletotrichum capsici in Capsicum. Plant Breeding. 124:206-208. https://doi.org/10.1111/j.1439-0523.2004.01065.x
  45. Park, H. K., Kim, B. S. and Lee, W. S. 1990. Inheritance of resistance to anthracnose (Colletotrichum spp) in pepper (Capsicum annuum L.) II. Genetic analysis of resistance to Colletotrichum dematium. J. Kor. Soc. Hort. Sci. 31:207-212.
  46. Park, K. S. and Kim, C. H. 1991. Five fungal species associated with anthracnose of red pepper in Korea. Phytopathology 81:1135.
  47. Park, K. S. and Kim, C. H. 1992. Identification, distribution and etiological characteristics of anthracnose fungi of red pepper in Korea. Korean J. Plant Pathol. 8:61-69.
  48. Park, W. M., Kim, S. H. and Ko, Y. H. 1989. Susceptibilization of a red pepper (Capsicum annuum) to Colletotrichum gloeosporioides Penz. in relation to the ripening of fruits. Kor. J. Plant Pathol. 5:262-270.
  49. Park, Y. K., Kim, B. D., Kim, B. S., Armstrang, K. C. and Kim, N. S. 1999. Keryotyping of the chromosome and physical mapping of the 5S rRNA and 18S-26S rRNA gene families in five different species in Capsicum. Genus & Genetic systems. 72:149-157.
  50. Pickersgill, B. 1988. The genus Capsicum: A multidisciplinary approach to the taxonomy of cultivated and wild plants. Biologisches Zentralblant 107:381-389.
  51. Poonpolgul, S. and Kumphai, S. 2007. Chilli Pepper anthracnose in Thailand. Country report. In: Oh, D. G and Kim, K. T. eds., p. 23. The first international symposium on Chilli Anthracnose. National Horticultural Research Institute, Rural Development Administration, Korea.
  52. Prince, J. P., Lackney, V. K., Angeles, C., Blauth, J. R. and Kyle, M. M. 1995. A survey of DNA polymorphism within the genus Capsicum and the fingerprinting of Pepper cultivars. Genome 38:224-231. https://doi.org/10.1139/g95-027
  53. Redondo, J. E. 1979. Busqueda de genotipos de chile resistance al hange Phytophthora capsici Leonion. Proc. Tropical Region. A. S. H. S. 23:220-224.
  54. Ribeiro, C. S., Boiteux, L. S., Amaro, G. B. and Reifschneider, F. J. B. 2008. Capsicum breeding at Embrapa vegetable crops, Brasilia, Brazil. In: Proceedings of the 19th International Pepper Conference, Sep. 7-10, Atlantic City, NJ, USA.
  55. Ryzhova, N. N. and Kochieva, E. Z. 2004. Analysis of microsatellite loci of the chloroplast genome in the genus Capsicum (Pepper). Russian J. Genetics 40:892-896. https://doi.org/10.1023/B:RUGE.0000039723.90902.b0
  56. SAGARPA. 2002. Programa agricola de los ciclos 0.1. 2001/2002. pv. 2002/2003. Servicio de Informacion estadistica Agroalinentaria presquera (SIAP). Secretaria de Agricultura Ganaderia, Desarrollo Rural, Pesca Alimentacion. Pagina web: www.sagarpa.gob.mx
  57. Shin, H. J., Chen, Z. J., Hwang, J. M. and Lee, S. G. 1999. Comparison of pepper anthracnose pathogens from Korea and China. Plant Pathol. J. 15:323-329.
  58. Smith, P. G. and Heiser, C. B. 1957. Taxonomy of Capsicum sinense Jacq. and the geographic distribution of the cultivated Capsicum species. Bul. Torrey Bot. Club 84:413-420. https://doi.org/10.2307/2482971
  59. Tong, N. and Bosland, P. W. 1999. Capsicum tovarii, a new member of the Capsicum baccatum complex. Euphytica 109:71-77. https://doi.org/10.1023/A:1003421217077
  60. Um, K. H. 1998. Conidia germination and appressorium formation of colletotrichum spp. isolate pepper fruits. MS Diss., Seoul National University. p. 93.
  61. Verma, M. L. 1973. Comparative studies on virulence of isolates of species of Colletotrichum parasitic on Chillies. Indian Phytopathology 26:28-31.
  62. Wall, M. M. and Bosland, P. W. 1993. The shelf-life of Chiles and Chile containing products. p. 487-500. In: G. Charalambous, ed., Shelf life studies of foods and bebverages, chemical, biological, and nutritional aspects. Elsevier Publishing, New York.
  63. Walsh B. H. and Hoot, S. B. 2001. Phylogenetic relationship of Capsicum (Solanaceae) using DNA sequences from two noncoding regions: The chloroplast atpB-rbc1 spacer region and nuclear waxy introns. Int. J. Plant Sci. 162:1409-1418. https://doi.org/10.1086/323273
  64. Walter, J. M. 1992. Colletotrichum diseases of perennial and other cash crops. In: Colletotrichum-Biology, Pathology, and Control. J. A. Bailey and M. J. Jeger, ed., pp. 167-185. CAB Intl., Wallingford, United Kingdom.
  65. Wei, X., Jeon, H. S., Han, K. S., Koh, Y. J. and Hur, J. S. 2008. Antifungal activity of lichen-forming fungi against Colletotrichum acutatum on Hot Pepper. Plant Pathol. J. 24:202-206. https://doi.org/10.5423/PPJ.2008.24.2.202
  66. Woodbury, J. E. 1980. Determination of Capsicum pungency by high pressure liquid chromatography and spectrofluorometric detection. J. Assn. Offic. Anal. Chem. 63:556-558.
  67. Yoon, J. B. and Park, H. G. 2001. Screening method for resistance to pepper fruit anthracnose; Pathogen sporulation, inoculation methods related to inoculums concentrations, and post-inoculation environments. J. Kor. Sco. Hort. Sci. 42:389-393.
  68. Yoon, J. B., Do, J. W., Yang, D. C. and Park, H. G. 2004. Interspecific cross compatibility among five domesticated species of Capsicum genus. J. Korean Soc. Hort. Sci. 45:324-329.

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