생명과학회지 (Journal of Life Science)

  • 제20권8호
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  • Pages.1214-1220
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Carbapenemase를 생산하는 imipenem 내성 세균의 특성 및 항생제 감수성

Characteristics and Antibiotic Susceptibility of Imipenem-Resistant Clinical Isolates Producing Carbapenemase

  • Choe, Han-Na (Department of Biology, Sunchon National University) ;
  • Park, Chul (Department of Biology, Sunchon National University) ;
  • Kim, Hyung-Rak (Department of Biology, Sunchon National University) ;
  • Baik, Keun-Sik (Department of Biology, Sunchon National University) ;
  • Kim, Se-Na (Department of Biology, Sunchon National University) ;
  • Seong, Chi-Nam (Department of Biology, Sunchon National University)
  • 투고 : 2010.05.28
  • 심사 : 2010.07.20
  • 발행 : 2010.08.30
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초록

대한민국 순천의 병원 입원 환자의 검체로부터 imipenem 내성 세균을 분리하였다. 54개의 분리균을 16S rRNA 유전자와 gyrB 유전자 염기서열 비교를 기초로 하여 계통분류학적으로 동정하였다. 분리균들은 Pseudomonas aeruginosa (30균주; 55.6%), Acinetobacter baumannii (21; 38.9%), Enterobacter hormaechei (2)와 Pseudomonas putida (2)에 속했다. 22개의 균주가 metallo-$\beta$-lactamase (MBL)를 생산하였으며 종별 구성은 다음과 같다; Acinetobacter baumannii 12균주, Pseudomonas aeruginosa 7균주, P. putida 2균주 그리고 Enterobacter hormaechei 1균주. 분리균들의 항생제 감수성은 디스크 확산법과 Vitek 을 이용하여 조사하였다. IMP 와 VIM 형의 metallo-$\beta$-lactamase를 생산하는 균주들은 OXA 와 SHV 형 $\beta$-lactamase를 생산하는 균주들에 비해 ceftazidime, aztreonam, amikacin과 gentamicin에 대한 내성율이 높았다.

Imipenem-resistant bacteria were isolated from clinical specimens taken from hospitalized patients in Suncheon, Korea. Fifty-four isolates were phylogenetically analyzed based on 16S rRNA gene and gyrB gene sequence comparisons. Isolates were affiliated with Pseudomonas aeruginosa (30 strains; 55.6%), Acinetobacter baumannii (21; 38.9%), Enterobacter hormaechei (2) and Pseudomonas putida (2). Twenty-two isolates produced metallo-$\beta$-lactamase (MBL); 12 Acinetobacter baumannii strains, 7 Pseudomonas aeruginosa strains, 2 P. putida strains and 1 Enterobacter hormaechei strain. Antibiotic susceptibility of the isolates was determined using the disc diffusion method and Vitek system. Strains producing metallo-$\beta$-lactamase (type IMP & VIM) were more resistant to antibiotics ceftazidime, aztreonam, amikacin and gentamicin than to strains producing OXA and SHV type of $\beta$-lactamase.

키워드

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피인용 문헌

  1. The Correlation between Toxin Genotype and Antibiotic Resistance in Methicillin ResistantStaphylococcus aureusIsolated from Clinical Specimen of Intensive Care Unit vol.48, pp.3, 2016, https://doi.org/10.15324/kjcls.2016.48.3.202
  2. Genotype Analyses of Methicillin Resistant Staphylococcus aureus Isolated from clinical specimens vol.16, pp.5, 2015, https://doi.org/10.5762/KAIS.2015.16.5.3315